Journal: Cells

Article Title: Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

doi: 10.3390/cells10112998

Figure Lengend Snippet: Doxorubicin increases FGF-1 and PDGF-B protein levels in adult heart ventricular cells. Mice were given intraperitoneal injections of saline (S) or doxorubicin (D) and their hearts were harvested after 24 h (Day 1) or 72 h (Day 3). Using Western blotting, lysates containing equal amounts of total protein from the ventricular tissue of the differently treated animals were tested for FGF-1 and PDGF-B protein levels. When standardized against GAPDH, the level of both proteins (FGF-1 and PDGF-B) was found to be significantly higher in the Dox-treated ventricles than in the saline treated ventricles by Day 3. * p < 0.05 vs. saline (S), One-way ANOVA with Tukey’s multiple comparisons test, N = 3 independent experiments for each group.

Article Snippet: For the analysis of growth factor effects, the wells contained 1% FBS (Low Serum) DMEM and 40 ng/mL of FGF-1 (rMuFGF1 TM , Gold Biotechnology, St. Louis, MO, USA), its neutralizing antibody (Santa Cruz sc-7910, 100 ng/mL), or both.

Techniques: Western Blot

Journal: Cells

Article Title: Regulation of Transplanted Cell Homing by FGF1 and PDGFB after Doxorubicin Myocardial Injury

doi: 10.3390/cells10112998

Figure Lengend Snippet: FGF-1 and PDGF-B increase embryonic ventricular cell migration, whereas their neutralizing antibodies decrease migration. 300 μL of 0.5 × 10 6 cells/mL serum-free cell suspensions were incubated for 22 h at 37 °C in polycarbonate membrane inserts with 8 μm pores, with surrounding wells containing 1% FBS DMEM and FGF-1, its antibody, or both ( A ) or PDGF-B, its antibody, or both ( B ). The inserts were then removed, migratory cells that had passed through the pores were washed and stained, and the cell stain was solubilized for measurement of absorbance. * p < 0.05 vs. all other groups; # p < 0.05 vs. all other groups, One-way ANOVA with Tukey’s multiple comparisons test, N = 3 independent experiments for each group.

Article Snippet: For the analysis of growth factor effects, the wells contained 1% FBS (Low Serum) DMEM and 40 ng/mL of FGF-1 (rMuFGF1 TM , Gold Biotechnology, St. Louis, MO, USA), its neutralizing antibody (Santa Cruz sc-7910, 100 ng/mL), or both.

Techniques: Migration, Incubation, Staining

Journal: Cancer Management and Research

Article Title: The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

doi: 10.2147/CMAR.S407123

Figure Lengend Snippet: The activating process of IL-36α.

Article Snippet: The CCK-8 kit, including human IL-36α/IL1F6 (IL36A) Kit, human IL-18, and IL-1β, was purchased from Solarbio, Beijing, China.

Techniques:

Journal: Cancer Management and Research

Article Title: The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

doi: 10.2147/CMAR.S407123

Figure Lengend Snippet: The expression of circulating ( A ) IL-36α, ( B ) AFP and ( C ) CEA transcribed. The X-axis represents different groups. The Y axis represents concentrations of these molecules (ng/mL). **** P <0.0001, ** P <0.01, * P <0.5.

Article Snippet: The CCK-8 kit, including human IL-36α/IL1F6 (IL36A) Kit, human IL-18, and IL-1β, was purchased from Solarbio, Beijing, China.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

doi: 10.2147/CMAR.S407123

Figure Lengend Snippet: The expression of IL-36α in HCC and non-cancer tissues. ( A ) Results of HE staining at ×200 magnification. ( B ) IL-36α expression levels in non-cancer liver tissue (N), well-differentiated HCC (W), moderately differentiated HCC (M), and poorly differentiated HCC (P) measured by ELISA. The mean concentration of each group is represented by A. The horizontal axis represents the different groups, and the vertical axis represents the concentration of IL-36α (ng/mL). The IL-36α concentration in W, M, and P was significantly higher than that in N (*P<0.05, **P<0.01).

Article Snippet: The CCK-8 kit, including human IL-36α/IL1F6 (IL36A) Kit, human IL-18, and IL-1β, was purchased from Solarbio, Beijing, China.

Techniques: Expressing, Staining, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Cancer Management and Research

Article Title: The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

doi: 10.2147/CMAR.S407123

Figure Lengend Snippet: Based on bioinformatics to investigate the prognosis of IL-36 in HCC. The correlation of IL-36R expression with prognosis of HCC patients. ( A ) IL-36R expression profile in different tumor types and normal (peri-tumor) tissues, with the height of each bar representing the median expression value. ( B ) Box plots showing IL-36R expression in liver hepatocellular carcinoma (LIHC) and normal (peri-tumor) tissues, obtained using the “Expression DIY” tab by GEPIA. The horizontal coordinate of T denotes LIHC tissues (n=369), while N denotes normal tissues (n=160). The color density of each block represents the median expression value, normalized by the maximum median expression value across all blocks. ( C ) The overall survival of LIHC patients with high and low IL-36R expression can be presented in the “Survival” tab, with a P value of 0.033 (<0.05).

Article Snippet: The CCK-8 kit, including human IL-36α/IL1F6 (IL36A) Kit, human IL-18, and IL-1β, was purchased from Solarbio, Beijing, China.

Techniques: Expressing, Blocking Assay

Journal: Cancer Management and Research

Article Title: The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

doi: 10.2147/CMAR.S407123

Figure Lengend Snippet: The expression of IL-36α in different cell lines was investigated. ( A ) The expression levels of IL-36α were measured in Lo2 (normal liver cell), Huh7 (well-differentiated HCC cell), and HepG2 (poor-differentiated HCC cell), using ELISA. Higher expression of IL-36α was observed mainly in hepatocellular carcinoma cells. ( B ) The viability of the cell lines (Lo2, Huh7, and HepG2) was assessed after treatment with different concentrations of IL-36α (750, 500, 250, 125, and 62.5 ng/mL) for 24 hours. The viability of cells decreased with increasing IL-36α concentrations, with the greatest effect observed at 500 ng/mL. ( C and D ) The concentration of the treatment group was significantly lower than that of the blank control group. The scratch healing rate of lateral migration of HepG2 cells was assessed using a scratch healing experiment (*P<0.05).

Article Snippet: The CCK-8 kit, including human IL-36α/IL1F6 (IL36A) Kit, human IL-18, and IL-1β, was purchased from Solarbio, Beijing, China.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay, Control, Migration

Journal: Cancer Management and Research

Article Title: The Pro-Tumor Biological Function of IL-36α Plays an Important Role in the Tumor Microenvironment of HCC

doi: 10.2147/CMAR.S407123

Figure Lengend Snippet: IL-36α suppressed IL-1β and IL-18 expression in HepG2 cells in vitro. To test this, we set up blank control groups and treatment groups to measure the expression of IL-18 and IL-1β in HepG2 cells. The treatment group was treated with IL-36α (500 ng/mL) for 24 hours, while the control group was only added DMEM.

Article Snippet: The CCK-8 kit, including human IL-36α/IL1F6 (IL36A) Kit, human IL-18, and IL-1β, was purchased from Solarbio, Beijing, China.

Techniques: Expressing, In Vitro, Control