Journal: BioMed Research International

Article Title: Curcumin Targets Both Apoptosis and Necroptosis in Acidity-Tolerant Prostate Carcinoma Cells

doi: 10.1155/2021/8859181

Figure Lengend Snippet: Effects of docetaxel and curcumin on cell viability, ROS level, and mitochondrial membrane potential. PC-3 and PC-3AcT cells were treated with the indicated concentrations of DTX or CCM in DMEM containing 3.8 μ M lactic acid for 48 h. Two human prostate epithelial cell lines (RWPE-1 and HPrEC) were incubated in lactic acid-free DMEM. (a) Cell viability was measured by MTT assay. (b) Cellular ROS levels were measured by staining cells with DCF-DA (10 μ M). (c) ΔΨ m was measured by staining cells with Rhodamine 123 (30 nM). ∗ P < 0.05 vs. respective PC-3 cells. DTX: docetaxel; CCM: curcumin; ΔΨ m: mitochondrial membrane potential.

Article Snippet: The human PC cell line PC-3 and two human prostate epithelial cell lines, RWPE-1 and HPrEC, were obtained from the American Type Culture Collection (ATCC).

Techniques: Membrane, Incubation, MTT Assay, Staining

Journal: BioMed Research International

Article Title: Curcumin Targets Both Apoptosis and Necroptosis in Acidity-Tolerant Prostate Carcinoma Cells

doi: 10.1155/2021/8859181

Figure Lengend Snippet: Apoptosis- and necroptosis-inducing effects of curcumin. PC-3 and PC-3AcT cells were treated with the indicated concentrations of CCM for 48 h. Two human prostate epithelial cell lines (RWPE-1 and HPrEC) were incubated in lactic acid-free DMEM. (a) The levels of apoptosis- and necroptosis-mediated proteins were assessed by Western blotting. (b) Nuclear morphology was assessed by staining with DAPI (scale bar = 5 μ m). (c) Cells were pretreated with necrostatin-1 (25 μ M) and Q-VD-Oph-1 (10 μ M) 2 h prior to treatment with CCM (40 μ M) for 48 h in DMEM containing lactic acid (3.8 μ M). Cell viability was measured by MTT assay. ∗ P < 0.05 vs. respective control cells. # P < 0.05 vs. respective PC-3 cells. CCM: curcumin.

Article Snippet: The human PC cell line PC-3 and two human prostate epithelial cell lines, RWPE-1 and HPrEC, were obtained from the American Type Culture Collection (ATCC).

Techniques: Incubation, Western Blot, Staining, MTT Assay, Control

Journal: Cell

Article Title: Suppression of Exosomal PD-L1 Induces Systemic Anti-tumor Immunity and Memory

doi: 10.1016/j.cell.2019.02.016

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: LnCap , ATCC , CRL-1740; RRID:CVCL_1379.

Techniques: Western Blot, Flow Cytometry, Control, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Recombinant, SYBR Green Assay, cDNA Synthesis, Red Blood Cell Lysis, Software