Journal: Journal of Anatomy

Article Title: The distribution of the proteoglycan FORSE‐1 in the developing mouse central nervous system

doi: 10.1111/joa.12907

Figure Lengend Snippet: List of primary antibodies used

Article Snippet: This confirmed that the FORSE‐1 antibody showed excellent immunoreactivity and a strong signal where present. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Antibody Species and clonality Isotype Manufacturers Dilution FORSE1 Mouse monoclonal IgM Developmental Studies Hybridoma Bank 1 : 200 Nestin Mouse monoclonal IgG Developmental Studies Hybridoma Bank 1 : 200 BLBP Rabbit polyclonal N/A Millipore 1 : 200 GAP‐43 Rabbit polyclonal N/A Thermo‐Fisher Scientific 1 : 500 TAG‐1 Mouse monoclonal IgM Thermo‐Fisher Scientific 1 : 200 β‐III Tubulin Mouse monoclonal IgG Thermo‐Fisher Scientific 1 : 200 Open in a separate window List of primary antibodies used Cell culture Cerebral cortex and spinal cords were dissected from E15 embryos and placed in ice‐cold calcium and magnesium‐free Hanks’ balanced salt solution (CMF HBSS) (Thermo‐Fisher Scientific, USA).

Techniques:

Journal:

Article Title: Maintenance of caspase-3 proenzyme dormancy by an intrinsic "safety catch" regulatory tripeptide

doi: 10.1073/pnas.111085198

Figure Lengend Snippet: Acidification disables the safety catch and promotes procaspase-3 activation. (a) Ac-DEVD-AMC cleavage activity in procaspase-3 expressing bacterial extracts after incubation in buffers at varying pH levels. (b) α-Caspase-3 immunoblot and corresponding Ac-DEVD-AMC cleavage activity in cytosolic extracts from the neuronal precursor cells NT2 treated as described in a.

Article Snippet: Lysates were prepared from the E. coli strain BL21(DE3) pLysS as described above or from the human neuronal precursor cell line NT2 (Stratagene) according to the procedure described ( 6 ).

Techniques: Activation Assay, Activity Assay, Expressing, Incubation, Western Blot