Journal: Cancer Medicine

Article Title: CUL4B promotes aggressive phenotypes of HNSCC via the activation of the Wnt/β‐catenin signaling pathway

doi: 10.1002/cam4.1960

Figure Lengend Snippet: Expression of CUL4B in human HNSCC. A, qRT‐PCR analysis of CUL4B mRNA in HNSCC tissues (tumor) and paired normal tissues (normal). B, qRT‐PCR analysis of CUL4B mRNA in male and female HNSCC tissues. C, CUL4B protein level in HNSCC tissues and paired normal tissues were assessed by Western blotting. D, Immunohistochemistry of CUL4B in nontumor and primary HNSCC tissue arrays. Scale bar: 50 μm. E, Kaplan‐Meier analysis of overall survival for patients with HNSCC. The analyses were conducted based on the immunohistochemistry of CUL4B and the survival information provided by the supplier. * P < 0.05

Article Snippet: The human HNSCC tissue microarrays (paraffin‐embedded), including follow‐up survival information, were provided by SuperBioChips.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemistry

Journal: Cancer Medicine

Article Title: CUL4B promotes aggressive phenotypes of HNSCC via the activation of the Wnt/β‐catenin signaling pathway

doi: 10.1002/cam4.1960

Figure Lengend Snippet: Effects of CUL4B on malignant phenotypes in HNSCC cells. A, Cell viability of JHU‐012 cells transfected with vector or CUL4B was analyzed by MTS assays at different time points. B, Cell viability of sh con or sh CUL4B JHU‐012 cells was analyzed by MTS assays at different time points. C, Effects of CUL4B overexpression on anchorage‐dependent colony formation. Scale bar: 10 mm. D, Effects of CUL4B knockdown on anchorage‐dependent colony formation. Scale bar: 10 mm. E, CUL4B overexpression regulated transwell cell invasion and F, Matrigel invasion. Scale bar: 50 μm. G, CUL4B knockdown regulated transwell cell invasion and H, Matrigel invasion. Scale bar: 50 μm. I, Expression of epithelial markers, E‐cadherin and ZO‐1, and mesenchymal markers, vimentin, was analyzed by Western blotting in vector or CUL4B‐transfected JHU‐012 cells. J, Expression of epithelial markers, E‐cadherin and ZO‐1, and mesenchymal markers, vimentin, was analyzed by Western blotting in sh con or sh CUL4B JHU‐012 cells. * P < 0.05; ** P < 0.01

Article Snippet: The human HNSCC tissue microarrays (paraffin‐embedded), including follow‐up survival information, were provided by SuperBioChips.

Techniques: Transfection, Plasmid Preparation, Over Expression, Knockdown, Expressing, Western Blot

Journal: Cancer Medicine

Article Title: CUL4B promotes aggressive phenotypes of HNSCC via the activation of the Wnt/β‐catenin signaling pathway

doi: 10.1002/cam4.1960

Figure Lengend Snippet: CUL4B enhances Wnt/β‐catenin signaling in HNSCC cells. A, mRNA level of β‐catenin, cyclin D1, c‐myc, and MMP7 in vector or CUL4B overexpressed JHU‐012 cells was determined by qRT‐PCR. B, mRNA level of β‐catenin, cyclin D1, c‐myc, and MMP7 in sh con or sh CUL4B JHU‐012 cells was determined by qRT‐PCR. C, Protein level of β‐catenin, cyclin D1, c‐myc, and MMP7 in the indicated group was determined by qRT‐PCR and Western blotting. (D) Levels of the active form of β‐catenin in JHU‐012 cells with knockdown or overexpression of CUL4B. E and F, The activity of TCF/β‐catenin reporter (TOP/FOP Flash) in CUL4B‐knockdown (E) and CUL4B‐overexpressing cells (F). * P < 0.05; ** P < 0.01

Article Snippet: The human HNSCC tissue microarrays (paraffin‐embedded), including follow‐up survival information, were provided by SuperBioChips.

Techniques: Plasmid Preparation, Quantitative RT-PCR, Western Blot, Knockdown, Over Expression, Activity Assay

Journal: Cancer Medicine

Article Title: CUL4B promotes aggressive phenotypes of HNSCC via the activation of the Wnt/β‐catenin signaling pathway

doi: 10.1002/cam4.1960

Figure Lengend Snippet: Wnt/β‐catenin signaling regulates CUL4B functions in HNSCC cells Effects of XAV‐939, on CUL4B‐enhanced cell proliferation (A), migration (B), and invasion (C). Scale bar: 50 μm. Cells were treated with XAV‐939 or DMSO during the migration and invasion assays. ** P < 0.01

Article Snippet: The human HNSCC tissue microarrays (paraffin‐embedded), including follow‐up survival information, were provided by SuperBioChips.

Techniques: Migration