Journal: Pancreas

Article Title: Stromal Fibrosis and Expression of Matricellular Proteins Correlate With Histological Grade of Intraductal Papillary Mucinous Neoplasm of the Pancreas

doi: 10.1097/mpa.0000000000000617

Figure Lengend Snippet: FIGURE 2. Area of positive staining revealed by immunohistochemistry in IPMN. Positive staining of the periductal stroma with anti–α-SMA (A), antiperiostin (B), and antigalectin-1 (C) at a magnification of 200. Positive staining of the acinar area with anti–α-SMA (D), antiperiostin (E), and antigalectin-1 (F) at a magnification of 100.

Article Snippet: The primary antibodies and dilutions employed were the following: anti–α-SMA (M0851; DAKO Japan, Tokyo, Japan, 1:50), antiperiostin (RD181045050; BioVendor, Czech Republic, 1:4000), and antigalectin-1 (sc-166618; Santa Cruz Biotechnology, Dallas, 1:40).

Techniques: Staining, Immunohistochemistry

Journal: Cellular and Molecular Bioengineering

Article Title: Remote-Controlled Gene Delivery in Coaxial 3D-Bioprinted Constructs using Ultrasound-Responsive Bioinks

doi: 10.1007/s12195-024-00818-x

Figure Lengend Snippet: Effect of varying the number of ultrasound pulses on ultrasound-controlled osteoblast cellular transfection in coaxially-bioprinted constructs. A Coaxially-bioprinted osteoblast-laden (hFOB 1.19) 4% alginate bioink containing GFP-coupled microbubbles before ultrasound, 0 hr post-ultrasound, and 48 hr post-ultrasound with varying ultrasound exposure (10, 40, or 80 pulses). B Number of transfected cells in bioprinted constructs at 48 hr post-ultrasound for varying numbers of ultrasound pulses (*p < 0.05, ****p < 0.0001, one-way ANOVA, Tukey’s multiple comparisons test, error bars denote standard deviation). C Diameter of zone containing transfected cells at 48 hr post-ultrasound for varying numbers of ultrasound pulses (one-way ANOVA, Tukey’s multiple comparisons test, error bars denote standard deviation)

Article Snippet: They were then suspended in complete media containing Human Osteoblast Media (Cell Applications, Inc.), 10% FBS (HyClone Characterized Fetal Bovine Serum CA Origin, Cytiva), and 1% pen-strep (penicillin-streptomycin, 10,000 μg/mL, Gibco) then concentrated to 3.85 × 10 7 cell/mL in complete media.

Techniques: Transfection, Construct, Standard Deviation

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults *

doi: 10.1074/mcp.M116.066720

Figure Lengend Snippet: ELISA validation of differentially expressed proteins. A, GPI-specific phospholipase 1 (GPLD1), B, Periostin (POSTN), C, Alpha-2-macroglobulin (A2M), D, Histidine rich glycoprotein (HRG). E, For comparison, boxplots showing the respective abundance patterns detected from the SWATH data using the extended library (GPLD1 and POSTN) and the local library (A2M and HRG). All error bars are standard deviation (SD). * Denotes significance ≤ 0.05; ** denotes significance ≤ 0.01.

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) validation We utilized the following ELISA kits according to the manufacturer's specifications: GPI-specific phospholipase D (GPLD1); (CSB-EL009721HU, Cusabio, Wuhan, China), Periostin (POSTN) (CSB-E16444H, Cusabio, Wuhan, China), Alpha-2-macroglobulin (A2M) (ab108888, Abcam, Cambridge, United Kingdom), Histidine rich glycoprotein (HRG) (CSB-E13159H, Cusabio, Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Comparison, Data-independent acquisition, Standard Deviation