Journal: Journal of Gastrointestinal Oncology

Article Title: The roles and mechanisms of APOL1 in the development of colorectal cancer

doi: 10.21037/jgo-24-275

Figure Lengend Snippet: Effect of APOL1 on the biological behavior of CRC cells. (A) qRT-PCR was used to determine the APOL1 mRNA levels in HCT116, SW1116, and NCM460 cells. (B) In HCT116 cells, qRT-PCR was used to determine the interference efficacy of shAPOL1#1/2/3. (C) CCK8 assay was used to evaluate the proliferative capacity of HCT116 and SW1116 cells. (D) The count of clones in HCT116 and SW1116 cells was determined using the colony formation test. Staining method: crystal violet. Magnification: ×100. (E) Wound healing test was used to assess the migratory capacity of HCT116 and SW1116 cells (magnification: ×40). (F) The Transwell test was used to determine HCT116 and SW1116 cells’ invasion ability (magnification: ×100). Staining method: crystal violet. Each group, n=3. *, P<0.05 vs. NCM460 group; **, P<0.01; ***, P<0.001 vs . sh-NC group. sh-NC, shRNA-negative control; sh-APOL1, shRNA-apolipoprotein L1; OD, optical density; CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; CCK8, Cell Counting Kit 8.

Article Snippet: Shanghai iCell Bioscience Inc. (Shanghai, China) provided the human normal colon epithelial cells NCM460 (iCell-h373) and CRC cell lines [HCT116 (iCell-h071) and SW1116 cells (iCell-h201)].

Techniques: Quantitative RT-PCR, CCK-8 Assay, Clone Assay, Staining, shRNA, Negative Control, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Genes & Genomics

Article Title: Tumor suppressive effect of scavenger receptor class A member 5 overexpression in colorectal cancer by regulating PI3K/AKT/mTOR pathway

doi: 10.1007/s13258-021-01139-3

Figure Lengend Snippet: Scavenger receptor class A member 5 (SCRAR5) expression in colorectal cancer (CRC) cells. The mRNA and protein levels of SCRAR5 in CRC cell lines (HT29, HCT116, SW480, and SW620) was significantly lower than that in normal HCoEpic cell line by qPCR ( A ) and western blotting ( B ), respectively

Article Snippet: Human normal colonic epithelial cell line HCoEpic and four CRC cell lines, including SW620, SW480, HT29, and HCT116, were purchased from Nanjing KeyGen Biotech.

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Toxic metabolite profiling of Inocybe virosa

doi: 10.1038/s41598-020-70196-7

Figure Lengend Snippet: ( A ) The dose–response effect of IV extract on NCM460 cells; IC 50 value—0.63 mg/mL, ( B ) the effect of IV extract on the morphology of NCM460 cells under phase contrast microscope (20 ×).

Article Snippet: The immortalized cell lines, NCM460 and Chang liver cell line were both obtained from National Centre for Cell Sciences (NCCS), Pune, India and grown at 37 °C in a humidified atmosphere of 5% CO 2 using MEM (Minimum Essential Media), supplemented with 10% FBS and antibiotic solution.

Techniques: Microscopy

Journal: Heliyon

Article Title: Development and validation of prognostic signatures of NAD+ metabolism and immune-related genes in colorectal cancer

doi: 10.1016/j.heliyon.2024.e34403

Figure Lengend Snippet: The Gene Ontology (GO) enrichment results of NAD + metabolism- and immune-related DEGs.

Article Snippet: We obtained human normal colonic epithelial cells (CCD814) and three human CRC cell lines (HCT-116, LOVO, and SW480) from iCell Bioscience, Inc. (Shanghai, China).

Techniques: Glycoproteomics, Membrane, Clinical Proteomics, Binding Assay, Activity Assay

Journal: Scientific Reports

Article Title: Identification of novel autophagy-related lncRNAs associated with a poor prognosis of colon adenocarcinoma through bioinformatics analysis

doi: 10.1038/s41598-021-87540-0

Figure Lengend Snippet: The qPCR result of seven lncRNAs in five COAD and normal colon mucosal epithelial cell lines. ( A ) qPCR result of AC027307.2; ( B ) qPCR result of AC073611.1; ( C ) qPCR result of MIR210HG; ( D ) qPCR result of AL161729.4; ( E ) qPCR result of LINC01063; ( F ) qPCR result of AC156455.1; ( G ) qPCR result of PCAT6.

Article Snippet: Normal human colon mucosal epithelial cell line (CCD-18Co) and the human COAD cell lines (SW480, LS174T, HCT116, DLD-1 and HT29) were purchased from iCell (Shanghai, China).

Techniques: