Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.

doi: 10.1158/1078-0432.CCR-06-1562

Figure Lengend Snippet: Fig. 1. Expression of CLIC4 is altered in several human cancer types. A, tumor array representing cDNAs derived from the ‘‘matched’’normal (N) and tumor (T) tissues (paired horizontally) of multiple human cancer types is probed with radioactively labeled CLIC4 or ubiquitin (inset) DNA probes. B, oncomine database (University of Michigan Comprehensive Cancer Center) was searched with‘‘CLIC4’’ as a query.Two examples of statistically significant changes (prostate/Lapointe/ Stanford University/P = 1.8 1021and lung/Bhattacharjee/Harvard Medical School/P = 3 109). Blue columns, normal tissues; red columns, cancerous tissues. Brackets,1.5 times interquartile range; dots, outliers; line within box, median; box boundaries, upper and lower quartiles.Two independent studies. C, tumor lysate dot blot representing 200 tumor samples (600 ng per spot) is probed with NH2-terminal CLIC4 antibody. ‘‘Matched’’normal (solid gray arrowhead) and tumor (solid black arrowhead) tissue samples are spotted vertically as a pair. Open circles are marker spots for an orientation.The experiment was repeated twice, and independent experiments with 10-fold less amount of lysate loaded per spot showed similar results.

Article Snippet: Immunohistochemistry, immunofluorescence, and tissue microarray staining Human tissue microarrays were obtained from multiple sources: TARP tissue arrays (National Cancer Institute), ‘‘matched’’ human tumor tissue arrays (Imgenex, San Diego, CA), Food and Drug Administration–standard normal and tumor tissue arrays (Biochain, Hayward, CA) and human cancer screen tissue arrays (Clinomics), prostate tumor arrays (Baylor Specialized Programs of Research Excellence), and human tissue arrays (Accumax) were used for CLIC4 expression and localization studies.

Techniques: Expressing, Derivative Assay, Labeling, Ubiquitin Proteomics, Dot Blot, Marker

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.

doi: 10.1158/1078-0432.CCR-06-1562

Figure Lengend Snippet: Fig. 2. CLIC4 expression and subcellular localization are altered in human tumors. Human normal and matched tumor (roman numerals, tumor stage) tissue sections representing (A) esophagus, (B) kidney, and (C) colon from the tissue microarrays are immunostained with anti-CLIC4 antibody and visualized by bright-field microscopy under 40 magnification. Inset, lower magnification (10). Black arrows, CLIC4 localized to the nucleus in the normal tissues. Similar staining patterns of CLIC4 were found in multiple human tumor types. Representative immunohistochemical stainings.

Article Snippet: Immunohistochemistry, immunofluorescence, and tissue microarray staining Human tissue microarrays were obtained from multiple sources: TARP tissue arrays (National Cancer Institute), ‘‘matched’’ human tumor tissue arrays (Imgenex, San Diego, CA), Food and Drug Administration–standard normal and tumor tissue arrays (Biochain, Hayward, CA) and human cancer screen tissue arrays (Clinomics), prostate tumor arrays (Baylor Specialized Programs of Research Excellence), and human tissue arrays (Accumax) were used for CLIC4 expression and localization studies.

Techniques: Expressing, Microscopy, Staining, Immunohistochemical staining