Journal: Molecular and Cellular Biology

Article Title: Structural Insights into Nox4 and Nox2: Motifs Involved in Function and Cellular Localization ▿ †

doi: 10.1128/MCB.01393-09

Figure Lengend Snippet: The Nox4 D loop facilitates interaction with p22phox Y121H. (A) H661 cells coexpressing Nox4 with an empty vector (EV), wild-type p22phox, or p22phox Y121H were analyzed for H2O2 production (error bars indicate SDs; n = 3) and expression of Nox4 and p22phox proteins. Actin served as a loading control. (B) Maturation of Nox2 and chimeras 12a (upper panel) and chimeras 3b, 4b, and 5b (lower panel) to a 91-kDa form and p22phox expression were determined by immunoblotting. Actin served as a loading control. (C) Analysis of Nox4, Nox2, and chimera localization using immunofluorescence. Transfected H661 cells were stained with anti-Nox4 (red, first and third panels) or anti-Nox2 (green, second panel) and anti-p22phox (wild-type p22phox and p22phox Y121H, in green for panels 1 and 3 and in red for panel 2). All images displayed are merged pictures of all three channels, with white indicating regions of colocalization. Scale bars represent 20 μm.

Article Snippet: Human H661 lung carcinoma cells (ATCC HTB-183) were cultivated in RPMI 1640, and Cos-p22phox cells ( 36 ), Cos-phox cells ( 27 ), and Cos-Nox4/p22phox cells were cultivated in Dulbecco modified Eagle medium (DMEM).

Techniques: Plasmid Preparation, Expressing, Control, Western Blot, Immunofluorescence, Transfection, Staining

Journal: Scientific reports

Article Title: Polyethylene glycol-phospholipid functionalized single-walled carbon nanotubes for enhanced siRNA systemic delivery.

doi: 10.1038/s41598-024-80646-1

Figure Lengend Snippet: Fig. 2. Significant gene silencing efficiency using SWNT-siRNA conjugates. (A) Graphical representation of SWNT-PL-PEG-S-S-siRNA. (B) Graphical representation of SWNT-PL-PEG-siRNA. (C, D and E) Evaluation of gene silencing effect by SWNT-PL-PEG-S-S-siGFP and SWNT-PL-PEG-siGFP conjugates in MCF-7, HEK-293T, H1299 and HeLa cells. Cells expressing stable green fluorescent protein (GFP) were treated with SWNT-PL-PEG-S-S-siGFP or SWNT-PL-PEG-siGFP conjugates at a concentration of 100 nM siGFP. The cell nuclei were stained with DAPI (blue). Microscopic observation was captured under 100x magnification. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to vehicle controls (Student’s t-test, p < 0.01).

Article Snippet: Cell lines and cell culture The human breast cancer cell line MCF-7, human non-small cell lung carcinoma line H1299, and human cervical cancer cells HeLa were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Concentration Assay, Staining

Journal: Scientific reports

Article Title: Polyethylene glycol-phospholipid functionalized single-walled carbon nanotubes for enhanced siRNA systemic delivery.

doi: 10.1038/s41598-024-80646-1

Figure Lengend Snippet: Fig. 3. Dual-silencing efficacy of siRNA-conjugated SWNT delivery system. Fluorescence microscopic images of co-expressed GFP and RFP in H1299 cells incubated with 100 nM of (A) SWNT-PL-PEG-S-S-siRNA or (B) SWNT-PL-PEG-siRNA conjugates targeting GFP (siGFP) or RFP (siRFP) or LUC (siLUC; negative control) alone, or both (dual targeting; siGFP + siRFP) for 72 h. The cell nuclei were stained with DAPI. The furthest right panel was the combined images. Microscopic observation was captured under 100x magnification.

Article Snippet: Cell lines and cell culture The human breast cancer cell line MCF-7, human non-small cell lung carcinoma line H1299, and human cervical cancer cells HeLa were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Fluorescence, Incubation, Negative Control, Staining

Journal: Scientific reports

Article Title: Polyethylene glycol-phospholipid functionalized single-walled carbon nanotubes for enhanced siRNA systemic delivery.

doi: 10.1038/s41598-024-80646-1

Figure Lengend Snippet: Fig. 4. Energy dependent cellular internalization mechanisms of SWNT-siRNA conjugates independent of ABCB1. Graph shows the mean relative luminescent units (RLU) of (A and B) luciferase expressing H1299 cells co-treated with SWNT only (without siLUC), SWNT-PL-PEG-S-S-siLUC or SWNT-PL-PEG- siLUC with or without sub-lethal concentrations of various endocytosis inhibitors. NaN3, chlorpromazine, genistein and m-βCD are inhibitors for energy-dependent cellular uptake, clathrin-mediated endocytosis, caveolae-dependent endocytosis and macro-pinocytosis, respectively. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to SWNT-siLUC (Student’s t-test, p < 0.01). (C) Western blot of ABCB1 and loading control GAPDH, cropped blots. Ectopic expression of ABCB1 (multiple drug resistance) protein in H1299-LUC cells was confirmed by the immunoblotting with increased expression of ABCB1 at 141 kDA, while GAPDH at 36 kDA served as the loading control. Full blots available in Supplementary Figure S2-S3. (D) Expression of ABCB1 did not affect the delivery of siRNA by the SWNT. Bars represent mean ± SD of at least 3 independent experiments. * represents statistical significance as compared to controls (Student’s t-test, p < 0.01).

Article Snippet: Cell lines and cell culture The human breast cancer cell line MCF-7, human non-small cell lung carcinoma line H1299, and human cervical cancer cells HeLa were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Luciferase, Expressing, Western Blot, Control

Journal: Cell Reports

Article Title: Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell

doi: 10.1016/j.celrep.2021.109364

Figure Lengend Snippet:

Article Snippet: Human: HC827 , ATCC , CRL-2868.

Techniques: Virus, Control, Recombinant, Transfection, Sequencing, Modification, Saline, RNAscope, Multiplex Assay, cDNA Synthesis, SYBR Green Assay, Infection, Software, Plasmid Preparation

Journal: Scientific Reports

Article Title: Implementation and Quality Control of Lung Cancer EGFR Genetic Testing by MALDI-TOF Mass Spectrometry in Taiwan Clinical Practice

doi: 10.1038/srep30944

Figure Lengend Snippet: Accuracy Test of MALDI-TOF MS in EGFR Mutation Testing by Traceable Biological Materials.

Article Snippet: NCI-H226 , Human lung squamous cell carcinoma cell line , ATCC , WT , No , 0.0 , No , 0.1 , No , 2.5 , ATCC CRL-5826.

Techniques: Mutagenesis, Plasmid Preparation, Clone Assay, Expressing