Journal: Nature Communications

Article Title: Lugdunin amplifies innate immune responses in the skin in synergy with host- and microbiota-derived factors

doi: 10.1038/s41467-019-10646-7

Figure Lengend Snippet: Proposed model of lugdunin-mediated skin protection. Lugdunin acts on different levels to protect against S. aureus skin infection: First it can directly inhibit and kill S. aureus . Secondly, it can cooperate with host-derived antimicrobial peptides (AMPs) such as hCAP18/LL-37 and the dermcidin-derived peptides DCD-1(L) to kill S. aureus . Additionally, on a third level of protection, lugdunin induces an innate immune response of the skin, which leads to the recruitment of phagocytic immune cells, which will clear potentially invading pathogens. Finally, this innate immune response can be highly amplified by factors derived from the skin commensal S. epidermidis . Blue, lugdunin; orange, DCD-1(L); multicolored, commensals

Article Snippet: The next day, the plate was washed three times using PBS + 0.5% BSA + 0.05% Triton X-100, followed by incubation with 100 µL primary antibody against hCAP18/LL-37 (HycultBiotech, Cat#HM2071, 1:120 in PBS + 0.5% BSA + 0.05% Triton X-100) at 37 °C for 1 h. After washing, incubation with 100 µL secondary antibody (Cell Signaling, Cat#7076S, 1:3000 in PBS + 0.5% BSA + 0.05% Triton X-100) followed at 37 °C for 1 h. Subsequently, plates were washed and 100 µL TMB substrate solution (Cell Signaling) was added.

Techniques: Infection, Derivative Assay, Amplification

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: LL37 is expressed in synovial compartment of PsA. (A) LL37 was measured by ELISA in synovial fluids of PsA ( N = 12) and control OA patients ( N = 11), and LL37 levels are shown as median with Interquartile Range (IQR). P-value is calculated by two-tailed Mann-Whitney U test * p < 0.05. (B) Confocal microscopy images of synovial tissues of PsA and OA patients stained for myeloperoxidase (MPO; red), LL37 (gray) (original magnification 63x). For PsA, 1 representative staining of 7 patients is shown. For OA, 1 representative staining of 4 patients is shown.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Two Tailed Test, MANN-WHITNEY, Confocal Microscopy, Staining

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: Anti-LL37 antibodies are present in synovial fluids of PsA. Synovial fluids of PsA and OA ( N = 11) patients were analyzed by ELISA for the presence of anti-LL37 (PsA, N = 19), anti-LL37cit (PsA, N = 21) and anti-LL37carb (PsA, N = 17). Antibody levels are shown as median with Interquartile Range (IQR). P -value is calculated by two-tailed Mann-Whitney U test, *** p < 0.0001. The mean+2 SD (standard deviation) of OA antibody reactivity to native LL37 or modified LL37 was used as cut-off (dotted line).

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY, Standard Deviation, Modification

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: Anti-LL37 levels in SF PsA correlate with disease activity and inflammation markers. SF PsA levels of anti-LL37 Abs ( N = 16) measured by ELISA as shown in Figure were correlated with Disease Activity Score (DAS44), C reactive Protein (CRP), Eritrocyte Sedimentation Rate (ESR) and number of swollen joints. The correlation between the two variables was assessed by Spearman correlation coefficient.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Sedimentation

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: Autoantibody reactivity to citrullinated and carbamylated LL37 in plasma of PsA and Pso patients. (A) Levels of anti-LL37cit ( N = 29) and anti-LL37carb ( N = 32) were measured in plasma of PsA, Pso (without PsA, N = 17) and healthy donors (HD, N = 14) by ELISA. Antibody levels are shown as median with Interquartile Range (IQR). P -value is calculated by two-tailed Mann-Whitney U test, * p < 0.05, *** p < 0.0001. The mean of HD antibody reactivity+2 SD was used as cut-off (dotted line). (B) Levels of PsA anti-LL37carb were correlated with Disease Activity Score (DAS44) by Spearman correlation coefficient and the significant P value by two-tailed Mann-Whitney U test.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY, Activity Assay

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: IgG - immune complexes deposition and colocalization with neutrophil-derived LL37 in synovia of PsA. Confocal microscopy images of synovial tissues of two PsA patients stained for LL37 (red), IgG-IC (green) (original magnification, 63x). The dotted white line in PsA#2 indicates the inset of the corresponding picture of the bottom panel. Data are representative of 7 PsA patients.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Derivative Assay, Confocal Microscopy, Staining

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: Complement C5a, C9, and GM-CSF in SF and plasma of PsA and Pso patients. (A) Content of C5a ( N = 19), C9 ( N = 19), and GM-CSF ( N = 21) were measured by ELISA in SF of PsA and OA ( N = 12). P -value is calculated by two-tailed Mann-Whitney U test, * p < 0.05, ** p < 0.01, *** p < 0.0001. (B) Plasma levels of C5a in PsA ( N = 25) and Pso (without PsA, N = 14), PsA C9 ( N = 18), and Pso ( N = 25), PsA GM-CSF ( N = 25) and Pso ( N = 16) were assessed by ELISA. All data in (A) and (B) are shown as as median with Interquartile Range (IQR). P -value is calculated by two-tailed Mann-Whitney U test, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: GM-CSF correlates with complement and anti-LL37 antibodies in PsA. (A) Spearman correlation analysis was performed between levels of GM-CSF and anti-LL37carb in SF PsA ( N = 17). (B) Spearman correlation analysis between GM-CSF and C5a, anti-LL37cit, and anti-LL37carb in plasma of PsA patients ( N = 23).

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: Circulating C5a correlates with anti-LL37 antibodies and disease activity. Spearman correlation analysis was performed between levels of C5a with anti-LL37cit ( N = 24), anti-LL37carb ( N = 24) and DAS44 in plasma of PsA ( N = 17).

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Activity Assay, Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: C9 staining in synovial tissues of PsA. Confocal microscopy images of synovial tissues of two PsA and one OA patients stained for LL37 (red) and C9 (green) (original magnification, 63x). The dotted white line in PsA#2 indicates the inset of the corresponding picture of the lower panel. Data are representative of 7 PsA patients and 4 OA subjects.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Staining, Confocal Microscopy

Journal: Frontiers in Immunology

Article Title: Anti-LL37 Antibodies Are Present in Psoriatic Arthritis (PsA) Patients: New Biomarkers in PsA

doi: 10.3389/fimmu.2018.01936

Figure Lengend Snippet: Type I IFN in the synovial compartment of PsA. (A) IFNa was measured by ELISA in SF of PsA ( N = 20) and OA ( N = 12) subjects. Data are shown as median with Interquartile Range (IQR). P -value is calculated by two-tailed Mann-Whitney U test *** p < 0.001. (B) Confocal microscopy images of synovial tissues of one PsA and one OA patient stained for LL37 (red) and MxA (green) (original magnification, 63x). Data are representative of 7 PsA patients and 4 OA subjects.

Article Snippet: LL37 levels of plasma and SF were measured by the Human antibacterial peptide LL37 ELISA Kit (Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, MANN-WHITNEY, Confocal Microscopy, Staining

Journal: European Journal of Dentistry

Article Title: The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

doi: 10.1055/s-0043-1761460

Figure Lengend Snippet: The SHED-IL10 ( A ) and SHED-LL37 ( B ) concentration on the basic condition.

Article Snippet: The antibody used was human IL-10 (human IL-10, BT Laboratory, Shanghai, China) and human LL37 (human LL317, IL-10, BT Laboratory, Shanghai, China).

Techniques: Concentration Assay

Journal: European Journal of Dentistry

Article Title: The EGCG and α-Mangosteen Stimulate SHED-IL10 and SHED-LL37 Metabolite Concentration

doi: 10.1055/s-0043-1761460

Figure Lengend Snippet: The SHED-IL10 ( A – C ) and SHED-LL37 ( E – F ) concentrations by two different epigallocatechin gallate (EGCG) concentrations and α-mangosteen. *** p < 0.001; **** p < 0.0001.

Article Snippet: The antibody used was human IL-10 (human IL-10, BT Laboratory, Shanghai, China) and human LL37 (human LL317, IL-10, BT Laboratory, Shanghai, China).

Techniques: