Journal: Cell reports. Medicine

Article Title: Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response.

doi: 10.1016/j.xcrm.2023.101234

Figure Lengend Snippet: Figure 6. Targeting CD36 synergistically promoted AG-mediated killing of PDAC in preclinical models (A) Visual presentation of subcutaneous xenograft murine PDAC tumor models (C57 mice) for each group. (B) Measurement of tumor volumes showed CD36 blockage synergistically promoted AG-mediated killing of PDAC in subcutaneous xenograft murine PDAC tumor models. (C) Measurement of tumor weights showed CD36 blockage synergistically promoted AG-mediated killing of PDAC in subcutaneous xenograft murine PDAC tumor models (n = 5). (D) Representative IHC staining showed Ki67 expression in subcutaneous xenografts treated with different regimens. (E) t-Distributed stochastic neighbor embedding (TSNE) analyses showed the clustering for CD36+ CD8+ T cells and GZMB+ CD8+ T cells. (F) Flow cytometry revealed that more CD8+ T cells infiltrated PDAC with NAC, while the percentage of CD36+ CD8+ T cells also increased (n = 5) (mean with standard deviation). (G) ELISA results showed the combination of AG and CD36 blockade significantly improved IFN-g and tumor necrosis factor a (TNF-a) levels intratumorally (n = 5). (H) Representative image of orthotopic murine models of PDAC. (I) Kaplan-Meier curve revealed the combination of CD36 blockade and AG significantly prolonged the survival interval of mice that received orthotopic PDAC cell transplantation (n = 10). Circle or square referred to a happened event (death or censored). Censored event means the mice is still alive at the time point that we ended follow-up. (J) CD36 blockade synergistically with AG regimens optimally narrowed the PDAC tumor size in a humanized PDX model (n = 10). (K) Representative IHC staining image of CD36-high and -low PDAC. (L) Kaplan-Meier curve showed increased CD36 expression predicted worse prognosis of PDAC patients with adjuvant AG chemotherapy. The statistical sig- nificance shown in this figure was detected using t test.

Article Snippet: The ELISA kits used in the present study were as follows: Human IFN-gamma ELISA Kit (absin, abs510007); Human IL-2 ELISA Kit (Boster, EK0397); Mouse TNF alpha ELISA Kit (absin, abs520010) and Mouse IFN- gamma ELISA Kit (absin, abs520007).

Techniques: Immunohistochemistry, Expressing, Flow Cytometry, Standard Deviation, Enzyme-linked Immunosorbent Assay, Transplantation Assay, Adjuvant

Journal:

Article Title: Intracellular expression of interleukin-4 and interferon-? by a Mycobacterium tuberculosis antigen-stimulated CD4 + CD57 + T-cell subpopulation with memory phenotype in tuberculosis patients

doi: 10.1111/j.1365-2567.2003.01785.x

Figure Lengend Snippet: Mycobacterial antigen-stimulated CD4+ T cells from tuberculosis (TB) patients. CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMC), by negative magnetic selection, and co-cultured with monocytes (CD14+ adherent cells). (a) The cells were stimulated with specific soluble protein concentrations of mycobacterial antigen (H37Rv strain), after which the cells were harvested and stained with anti-CD69 fluorescent antibody. The black circles denote the mean value and bars indicate the standard error from three independent experiments. (b) Intracellular cytokines in antigen-stimulated CD4+ T cells from TB patients. Representative plot of two regions identified by positive or negative fluorescence to CD57 staining versus forward angle scatter. Region 1 (R1) includes CD4+CD57− T cells, whereas region 2 (R2) includes CD4+ CD57+ T cells. Arrows in histograms indicate the immunofluorescence of interferon-γ (IFN-γ) and interleukin-4 (IL-4) on the gated cells. Data are representative of five independent experiments.

Article Snippet: Biotin-labelled rat anti-mouse IgM, and FITC-labelled antibodies to human CD14, CD19 and IFN-γ, were from Serotec Inc. (Raleigh, NC).

Techniques: Isolation, Selection, Cell Culture, Staining, Fluorescence, Immunofluorescence

Journal:

Article Title: Intracellular expression of interleukin-4 and interferon-? by a Mycobacterium tuberculosis antigen-stimulated CD4 + CD57 + T-cell subpopulation with memory phenotype in tuberculosis patients

doi: 10.1111/j.1365-2567.2003.01785.x

Figure Lengend Snippet: Percentage of CD4 + T cells positive to intracellular cytokines

Article Snippet: Biotin-labelled rat anti-mouse IgM, and FITC-labelled antibodies to human CD14, CD19 and IFN-γ, were from Serotec Inc. (Raleigh, NC).

Techniques: