Journal: Genes and immunity

Article Title: Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.

doi: 10.1038/gene.2009.72

Figure Lengend Snippet: Figure 4 Peripheral blood mononuclear cells (PBMCs), keratino- cytes and HepG2 cells express interferon (IFN)-lR1 and IL-10R2 protein. Protein expressions of IFN-l receptor components and of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were analyzed in the cell lysates of PBMCs from three donors as well as of keratinocytes and HepG2 cells using the western blot technique.

Article Snippet: For the detection of IFN-l receptor components, we incubated blots with anti-IFNlR1 pAbs (50 ng ml 1; Sigma-Aldrich), anti-IL-10R2 pAbs (600 ng ml 1; R&D Systems) and anti-GAPDH mAb (200 ng ml 1; clone 6C5; Millipore), followed by peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H and L), rabbit anti-goat IgG (H and L) and goat antimouse IgG (H and L) (all from Dianova) incubation, respectively, and final ECL detection.

Techniques: Western Blot

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Systemic Antibiotic Therapy Reduces Circulating Inflammatory Dendritic Cells and Treg-Th17 Plasticity in Periodontitis.

doi: 10.4049/jimmunol.1900046

Figure Lengend Snippet: Increased expression of IL-1R1 and IL-6Rα in CD4+Foxp3+ T cells in PD patients. CD4+ T cells were stimulated as in figure 3 in the presence and absence of IL-1β+IL-6. (A and B) Frequencies of C4+Foxp3+ T cells expressing IL-1R1 was increased in non-AB (n = 6–9) patients compared to healthy controls (HC) (n = 8) and AB (n = 5–6) subjects at day6 (A) and final (B) time points (Data are mean ± SEM, *p<0.05, **p<0.01, ***p<0.001). (C and D) Graphs depicts the Spearman’s positive correlation of Foxp3+IL-1R1+ population with %IL17A+Foxp3+ T cells (C) and Th17 cells (D) at final time point in response to IL1-β and IL-6 in non-AB group (n=6). (E and F) Graph shows the percentage of C4+Foxp3+ T cells expressing IL6Rα in healthy controls (HC) (n = 8), non-AB (n = 6–9), and AB (n = 5–6) subjects at day6 (E) and final (F) time points (Data are mean ± SEM, *p<0.05, **p<0.01). (G) Graph depicts the Spearman’s positive correlation between the Foxp3+IL-6Rα+ population and %IL17A+Foxp3+ T cells at final time point in response to IL1-β and IL-6 in non-AB group (n=9). (H) Increased expression of IL-1R1 in IL-17A+Foxp3+ Treg compared to conventional Treg and Th17 populations in periodontitis (PD) patients (Data are mean ± SEM, *p<0.05, **p<0.01, ***p<0.001). (I) Increased expression of IL-6Rα in IL-17A+Foxp3+ Treg compared to conventional IL17A-Foxp3+ Treg (Data are mean ± SEM, *p<0.05).

Article Snippet: Conjugated antibodies against the following were from R&D systems: RORγT (clone AFKJS-9) and IL-1R1 (Catalog number FAB269F).

Techniques: Expressing