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Image Search Results
Journal: The Journal of Biological Chemistry
Article Title: Human Memory, but Not Naive, CD4 + T Cells Expressing Transcription Factor T-bet Might Drive Rapid Cytokine Production
doi: 10.1074/jbc.M114.608745
Figure Lengend Snippet: Kinetics of cytokine secretion by activated naive and memory CD4+ T cells. Naive and memory CD4+ T cells were isolated from CD4+ T cells by MACS microbeads, and the purity of naive or memory CD4+ T cells was more than 97% as determined by flow cytometry (a). Naive and memory CD4+ T cells were stimulated with (open histogram) or without (shaded histogram) PMA and ionomycin in the presence of BFA for 6 h. Cells were stained by intracellular staining and analyzed by FACS (b). Purified naive and memory CD4+ T cells were stimulated with immobilized anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) for 0 to 72 h, and the concentrations of cytokines IFN-γ, IL-2, and TNF-α were detected by ELISA (c). Data are representative of three separate experiments with similar results.
Article Snippet: Human ELISA kits for cytokines IFN-γ, IL-2, and
Techniques: Isolation, Flow Cytometry, Staining, Purification, Enzyme-linked Immunosorbent Assay
Journal: Oncotarget
Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples
doi: 10.18632/oncotarget.9406
Figure Lengend Snippet: Highly concordant results between replicate measurements of AIB1 ( A1 , B1 ), EIF5A2 ( A2 , B2 ), and NMP22 ( A3 , B3 ) were obtained in the training and independent validation sets.
Article Snippet: ELISA kits of
Techniques: Biomarker Discovery
Journal: Oncotarget
Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples
doi: 10.18632/oncotarget.9406
Figure Lengend Snippet: The protein levels of AIB1 ( A1 , B1 ), EIF5A2 ( A2 , B2 ), and NMP22 ( A3 , B3 ) are substantially elevated in BCa urine samples when compared with the non-cancer urine samples in the training and independent validation sets ( p < 0.0001 for both). The results in all groups are presented as the mean and SD. Significance ( p < 0.05) was assessed using the Mann–Whitney U test.
Article Snippet: ELISA kits of
Techniques: Biomarker Discovery, MANN-WHITNEY
Journal: Oncotarget
Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples
doi: 10.18632/oncotarget.9406
Figure Lengend Snippet: Association of urinary levels of the three markers with selected patient characteristics
Article Snippet: ELISA kits of
Techniques: Biomarker Discovery
Journal: Oncotarget
Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples
doi: 10.18632/oncotarget.9406
Figure Lengend Snippet: Comparisons of the diagnostic performance by the model and individual AIB1, EIF5A2 or NMP22 in the training cohort A. and independent validation set B. .
Article Snippet: ELISA kits of
Techniques: Diagnostic Assay, Biomarker Discovery
Journal: Oncotarget
Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples
doi: 10.18632/oncotarget.9406
Figure Lengend Snippet: Logistic regression analysis of biomarkers in voided urine of the training and independent validation sets
Article Snippet: ELISA kits of
Techniques: Biomarker Discovery
Journal: International Journal of Molecular Sciences
Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells
doi: 10.3390/ijms25052806
Figure Lengend Snippet: Determination of the optimum concentrations of PMA, AEPS, and captopril for HUVEC treatments based on ACE activity. ( A ) ACE activity in HUVECs induced with 100–300 nM PMA, ( B ) ACE activity in PMA-induced HUVECs upon treatment with different concentrations of AEPS (100–300 µg/mL), and ( C ) ACE activity in PMA-induced HUVECs upon treatment with different concentrations of captopril (0.02–0.06 µM). Values are expressed as mean ± SEM, n = 6. * p < 0.05, ** p < 0.01 compared with the untreated control, # p < 0.05, ## p < 0.01, ### p < 0.001 compared with the PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.
Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and
Techniques: Activity Assay
Journal: International Journal of Molecular Sciences
Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells
doi: 10.3390/ijms25052806
Figure Lengend Snippet: Effects of AEPS treatment on ( A ) ACE mRNA expression and ( B ) ACE protein levels in HUVECs induced with PMA. Values are expressed as mean ± SEM, n = 6. *** p < 0.001 compared with control, ## p < 0.01, ### p < 0.001 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.
Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and
Techniques: Expressing
Journal: International Journal of Molecular Sciences
Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells
doi: 10.3390/ijms25052806
Figure Lengend Snippet: Effects of AEPS treatment on ACE activity in PMA-induced HUVECs. Values are expressed as mean ± SEM, n = 6. ** p < 0.01 compared with control. ## p < 0.01 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.
Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and
Techniques: Activity Assay
Journal: International Journal of Molecular Sciences
Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells
doi: 10.3390/ijms25052806
Figure Lengend Snippet: Effects of AEPS treatment on angiotensin II protein levels in PMA-induced HUVEC. Values are expressed as mean ± SEM, n = 6. *** p < 0.001 compared with control. # p < 0.05, ### p < 0.001 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; PMA, phorbol 12-myristate-13-acetate.
Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and
Techniques:
Journal: International Journal of Molecular Sciences
Article Title: Piper sarmentosum Roxb. Inhibits Angiotensin-Converting Enzyme Activity in Phorbol 12-Myristate-13-Acetate-Induced Endothelial Cells
doi: 10.3390/ijms25052806
Figure Lengend Snippet: Effect of AEPS on ( A ) AT1R and ( B ) AT2R mRNA expressions in PMA-induced HUVEC. Values are expressed as mean ± SEM, n = 6. * p < 0.05, ** p < 0.01 compared with control. ## p < 0.01, ### p < 0.001 compared with PMA group. ACE, angiotensin-converting enzyme; AEPS, aqueous extract of Piper sarmentosum leaves; AT1R, angiotensin II type 1 receptor; AT2R, angiotensin II type 2 receptor; PMA, phorbol 12-myristate-13-acetate.
Article Snippet: The ACE and Ang II protein levels in HUVECs were determined using Human Ang II and
Techniques: