Journal: Stem Cells International

Article Title: Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation

doi: 10.1155/2012/658356

Figure Lengend Snippet: Differentiation assay of Pl/Mb-MSCs in comparison to BM-MSCs and cytokines expression. (a) Representative differentiation of Pl/Mb-MSCs passage 4 is shown. Cells were kept in induction medium (differentiation) or control standard medium (control). (a–d) Osteogenic and adipocyte differentiation and control for BM-MSCs. (f–h) Osteogenic and adipocyte differentiation and control for Mb-MSCs. (b) Cytokine expressions of Mb-MSCs and BM-MSCs using the proteome profiler. (c) Quantification of cytokine optical density. Measurements were obtained with image J software (NIH).

Article Snippet: 200 μ g of protein was loaded on R&D system Human Cytokine Antibody Array panel A (R&D system, number ARY005) according to manufacturer's instructions.

Techniques: Differentiation Assay, Comparison, Expressing, Control, Software

Journal: Nature Communications

Article Title: 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

doi: 10.1038/s41467-018-02969-8

Figure Lengend Snippet: HBV-infected 3D PHH cultures predict human host responses. a Cytokine XL antibody array of uninfected 3D PHH cultures and cultures infected with 100 GE/cell patient-derived HBV for 10 days. b – g Comparison of the longitudinal protein expression values of b IL-8, c MIP-3α, d CXCL10, e VEGF, f Serpin E1, and g MCP-1 between HBV-infected patients, healthy controls, and 3D PHH cultures infected with patient-derived HBV (100 GE/cell) as well as correlation of each cytokine with HBsAg (HBV-infected patients and infected 3D PHH cultures) and HBeAg (infected 3D PHH cultures) levels. Data shown are mean ± SD of three independent experiments. p -values calculated by two-way ANOVA with Bonferroni post-test

Article Snippet: To quantify the expression of cytokines and chemokines, a Proteome ProfilerTM Human XL Cytokine Array Kit, a membrane-based antibody array for the determination of relative levels of 102 human cytokines and chemokines, was used according to the manufacturer’s protocol (R&D Systems, Inc., USA).

Techniques: Infection, Ab Array, Derivative Assay, Comparison, Expressing

Journal: Nature Communications

Article Title: 3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection

doi: 10.1038/s41467-018-02969-8

Figure Lengend Snippet: Kupffer cells do not alter HBV infection kinetics in 3D PHH cultures. a Cytokine XL antibody array of 3D PHH and 3D PHH/KC co-cultures (ratio 10:1) 13 days post-seeding. b Longitudinal osteopontin protein levels in 3D PHH and 3D PHH/KC co-cultures, determined by Luminex. c , d Response of 3D PHH and 3D PHH/KC co-cultures to 1 μg/mL LPS added 11 days post-seeding as determined by measurement of c IL-6 and d TNF-α 24 h post stimulation. e Acute phase response elicited by KC following infection with patient-derived HBV (100 GE/cell), determined by measurement of C-reactive protein using Luminex. f – k Comparative infection of f , h , i , k PHH and g , h , j , k PHH/KC co-cultures with patient-derived HBV (100 GE/cell) as determined by cumulative secretion of f , g HBsAg and i , j HBeAg and intracellular accumulation of h subgenomic HBV RNA and k pregenomic RNA. l , m Transcription of l type I and III interferon and m ISG following 10 days of infection of PHH or PHH/KC co-cultures with patient-derived HBV (100 GE/cell) in the absence or presence of 1000 IU/mL exogenous IFNα. Data shown are mean ± SD of four independent experiments

Article Snippet: To quantify the expression of cytokines and chemokines, a Proteome ProfilerTM Human XL Cytokine Array Kit, a membrane-based antibody array for the determination of relative levels of 102 human cytokines and chemokines, was used according to the manufacturer’s protocol (R&D Systems, Inc., USA).

Techniques: Infection, Ab Array, Luminex, Derivative Assay

Journal: American Journal of Cancer Research

Article Title: Tolypothrix Dichloromethane Ethylacetate fraction (TDEF) inhibits cisplatin resistance H357 cell through PI3K/AKT/beta-catenin pathway

doi: 10.62347/JTNQ4812

Figure Lengend Snippet: A. Oral cancer cell line H357 sensitive and H357cisR was seeded on 6 well plates and treated with different concentrations 5, 10, and 15 ug mL-1. With respect to time, the cell morphology changes and becomes rounded in shape, and at higher concentration cell starts to detach from the surface. B. H357CisR cells were treated with indicated dose of TDEF for 48 hrs after which cell death was determined by annexin V/7AAD assay using flow cytometer. The dot plots represent the percentage of early apoptotic (lower right) and late apoptotic cells (upper right). C. Bar diagrams indicate the percentage of cell death; black color portion indicates percentage of early apoptosis and grey color indicates percentage of late apoptosis occur with respective treated groups. D. TDEF treated H357cisR cell pellet was fixed with 70% ethanol and stained with propidium iodide and the cell distribution in different cell cycle phases was analyzed by FACS. Cell cycle arrest was shown highest at 10 ug mL-1 but the cell with the higher concentration of extract has experience apoptosis and necrosis. The percentage of the G1 population was found to be 65.1%, and the control G1 cell population was 47.8%.

Article Snippet: Protein array analysis Human apoptosis array analysis was performed using a proteome profiler apoptotic array kit (ARY009; R & D system.

Techniques: Concentration Assay, Flow Cytometry, Staining, Control

Journal: American Journal of Cancer Research

Article Title: Tolypothrix Dichloromethane Ethylacetate fraction (TDEF) inhibits cisplatin resistance H357 cell through PI3K/AKT/beta-catenin pathway

doi: 10.62347/JTNQ4812

Figure Lengend Snippet: A. The Pp53 and P21 signalling pathways are strongly activated by TDEF treatment. The human apoptosis proteome profiler array used a protein extract (400 µg). The intensities of each array spot were visualized and further quantified using an image. B. The graph shows the relative fold change of proteins with significant differences upon TDEF treatment, setting 1 for control (no treatment of TDEF). Protein levels with higher than ± 2 folds are considered candidates for TDEF-induced cell death.

Article Snippet: Protein array analysis Human apoptosis array analysis was performed using a proteome profiler apoptotic array kit (ARY009; R & D system.

Techniques: Control