Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Caffeic acid selectively eliminates teratogenic human-induced pluripotent stem cells via apoptotic cell death.

doi: 10.1016/j.phymed.2022.154144

Figure Lengend Snippet: Fig. 3. Increase of caspase activities by CAA treatment in iPSCs. (A) Apoptosis-related proteins were detected by Western blotting in CAA-treated iPSCs and hDFs. Relative band intensities were calculated after normalization to β-actin expression. (B) iPSCs and hDFs were treated with 50 and 100 μM CAA for 24 h, and the caspase-3, −8, and −9 activities were determined. Relative caspase activity compared with CAA-untreated iPSCs and hDFs was expressed as the means ± SD (n = 3). (C) iPSCs were pre-treated with or without 10 μM of caspase-3 inhibitor (z-DEVD), caspase-8 inhibitor (z-IETD), caspase-9 inhibitor (z-LEHD), and pan-caspase inhibitor (z-VAD) for 30 min and then treated with 50 and 100 μM CAA. DMSO was treated as vehicle control. After 24 h, cells were stained with crystal violet solution and relative cell viability compared with CAA-untreated control iPSCs were expressed as the means ± SD (n = 3). **p < 0.01 vs. CAA-untreated control. ##p < 0.01 vs. Inhibitor-untreated control. Scale bar = 100 μM.

Article Snippet: The expression profile of human apoptosis-related proteins in CAA-treated or -untreated iPSCs was analyzed using a Proteome Profiler Human Apoptosis Array Kit A. Kim et al. Phytomedicine 102 (2022) 154144 (ARY009, R&D System, Minneapolis, MN, USA) according to the manufacturer’s instruction.

Techniques: Western Blot, Expressing, Activity Assay, Control, Staining

Journal: RSC Advances

Article Title: pH-responsive niosome-based nanocarriers of antineoplastic agents †

doi: 10.1039/d4ra01334d

Figure Lengend Snippet: Changes in expression levels of apoptosis-related proteins in T-24 cells following treatment with: curcumin (B); Tw60 : Sp60 : Chol – S4 (C); Tw60 : Sp60 : Chol : HD-PAA 17 – S19 (D), as compared to untreated control (A). Cells were exposed to equieffective concentrations (IC 50 ) of free curcumin and its niosome formulations for 24 h, following which a human proteome profiler assay was performed according to manufacturer's instructions. Further densitometric analysis of the array spots was conducted using ImageJ software and the most prominent changes in the proteome were ex-pressed graphically (E). Legend: 1 – bad; 2 – TRAILR1, 3 – TRAILR2; 4 – bcl-2; 5 – bcl- x ; 6 – fas, CD95; 7 – pro-caspase 3; 8 – HIF-1α; 9 – HMOX1 (antiapoptotic); 10 – cIAP1; 11 – HSP60; 12 – survivin; 13 – claspin; 14 – HSP70; 15 – TNF RI; 16 – XIAP; 17 – HTRA/Omi.

Article Snippet: Proteome Profiler Human Apoptosis Array Kit, R&D Systems and a Proteome Profiler Human Cytokine Array Kit, R&D Systems were used for the parallel determination of multiple apoptosis- and inflammation-related protein endpoints.

Techniques: Expressing, Control, Software

Journal: RSC Advances

Article Title: pH-responsive niosome-based nanocarriers of antineoplastic agents †

doi: 10.1039/d4ra01334d

Figure Lengend Snippet: Changes in expression levels of apoptosis-related proteins in MJ cells following treatment with: curcumin (B); Tw60 : Sp60 : Chol – S4 (C); Tw60 : Sp60 : Ch : HD-PAA 17 – S19 (D), as compared to untreated control (A). Cells were exposed to equieffective concentrations (IC 50 ) of free curcumin and its niosome formulations for 24 h, following which a human proteome profiler assay was performed according to manufacturer's instructions. Further densitometric analysis of the array spots was conducted using ImageJ software and the most prominent changes in the proteome were ex-pressed graphically (E). Legend: 1 – bcl-2; 2 – bcl- x ; 3 – HIF-1α; 4 – phospho p53; 5 – cIAP1; 6 – cIAP2; 7 – survivin; 8 – claspin; 9 – HSP70; 10 – XIAP.

Article Snippet: Proteome Profiler Human Apoptosis Array Kit, R&D Systems and a Proteome Profiler Human Cytokine Array Kit, R&D Systems were used for the parallel determination of multiple apoptosis- and inflammation-related protein endpoints.

Techniques: Expressing, Control, Software

Journal: RSC Advances

Article Title: pH-responsive niosome-based nanocarriers of antineoplastic agents †

doi: 10.1039/d4ra01334d

Figure Lengend Snippet: Changes in expression levels of inflammation-related proteins in T-24 cells following treatment with: curcumin (B); Tw60 : Sp60 : Chol – S4 (C); Tw60 : Sp60 : Ch : HD-PAA 17 – S19 (D), as compared to untreated control (A). Cells were exposed to equieffective concentrations (IC 50 ) of free curcumin and its niosome formulations for 24 h, following which a human cytokine profiler assay was performed according to manufacturer instructions. Further densitometric analysis of the array spots was conducted using ImageJ software and the most prominent changes in the proteome were expressed graphically (E). Legend: 1 – MIF; 2 – PAI1; 3 – G-CSF; 4 – ICAM-1; 5 – IL-6; 6 – IL-18; 7 – IL-8; 8 – CXCL1; 9 – IL-1α; 10 – IL-1β.

Article Snippet: Proteome Profiler Human Apoptosis Array Kit, R&D Systems and a Proteome Profiler Human Cytokine Array Kit, R&D Systems were used for the parallel determination of multiple apoptosis- and inflammation-related protein endpoints.

Techniques: Expressing, Control, Software

Journal: RSC Advances

Article Title: pH-responsive niosome-based nanocarriers of antineoplastic agents †

doi: 10.1039/d4ra01334d

Figure Lengend Snippet: Changes in expression levels of inflammation-related proteins in MJ cells following treatment with: curcumin (B); Tw60 : Sp60 : Ch – S4 (C); Tw60 : Sp60 : Ch : PAA 17 – S19 (D), as compared to untreated control (A). Cells were exposed to equieffective concentrations (IC 50 ) of free curcumin and its niosome formulations for 24 h, following which a human cytokine profiler assay was performed according to manufacturer instructions. Further densitometric analysis of the array spots was conducted using ImageJ software and the most prominent changes in the proteome were expressed graphically (E). Legend: 1 – CCL2/MCP-1; 2 – CXCL11/I-TAC; 3 – CXCL12/SDF-1; 4 – IL-13; 5 – IL-16; 6 – MIF; 7 – SERPIN E1/PAI-1; 8 – CCL5/RANTES; 9 – GM-CSF; 10 – IL-5; 11 – ICAM1/CD54; 12 – IL-6; 13 – IL-18; 14 – IL-21; 15 – IL-1α; 16 – IL-32α.

Article Snippet: Proteome Profiler Human Apoptosis Array Kit, R&D Systems and a Proteome Profiler Human Cytokine Array Kit, R&D Systems were used for the parallel determination of multiple apoptosis- and inflammation-related protein endpoints.

Techniques: Expressing, Control, Software

Journal: Experimental and Therapeutic Medicine

Article Title: Insulin-like growth factor-binding proteins play a significant role in the molecular response to imatinib in chronic myeloid leukemia patients

doi: 10.3892/etm.2019.8364

Figure Lengend Snippet: Results of apoptosis antibody array data. (A) Heat maps of differentially expressed proteins between K562 and K562/G cells, the cut-off value was set as follows: Fold-change <1.2 or <0.83, fluorescent value >150 and P<0.05 (K562/G VS K562). (B) Kyoto Encyclopedia of Genes and Genomes pathway analysis of differentially expressed proteins.

Article Snippet: An apoptosis antibody array (Ray Biotech, Inc.) was used to explore the differences in the apoptotic protein profile between K562 and K562/G cells.

Techniques: Ab Array

Journal: International Journal of Molecular Sciences

Article Title: Association of SNPs in the PAI1 Gene with Disease Recurrence and Clinical Outcome in Bladder Cancer

doi: 10.3390/ijms24054943

Figure Lengend Snippet: PAI1 rs7242 is involved in the resistance to apoptosis induced by cisplatin. ( A ) Protein and mRNA expression of PAI1 clones in stable transfectants in UROtsa and 5637 cell lines. ( B ) Apoptosis assays using RealTime-Glo™ Annexin V Apoptosis and Caspase-Glo ® 3/7 Assays. ( C ) Profiling the expression of multiple proteins associated with apoptosis induced by cisplatin using a membrane-based sandwich immunoassay. ( D ) mRNA expression levels of ATR-CLSPN-CHEK1 pathway. ( E ) Silencing CLSPN by siRNA in parental UROtsa and 5637 cell lines. ( F ) Cell survival assay after cisplatin treatment. ( G ) Apoptosis assay after cisplatin treatment. Bars represent SE. ** p < 0.01; *** p < 0.001, **** p < 0. 0001.

Article Snippet: To investigate how PAI1 might inhibit apoptosis, we used an array (Human Apoptosis Array Kit; ARY 009, Promega, Madison, WI, USA) which monitors 35 apoptosis-related proteins.

Techniques: Expressing, Clone Assay, Membrane, Clonogenic Cell Survival Assay, Apoptosis Assay