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Image Search Results
Journal: CNS Neuroscience & Therapeutics
Article Title: ANGPT2 /Tie2 Enhances H3K18la ‐Mediated Macrophage M2 Polarization to Promote Endothelial Cell Proliferation in the Chronically Ischaemic Brain
doi: 10.1002/cns.70879
Figure Lengend Snippet: Results of flow cytometry, western blotting, and qPCR for determining the macrophage polarization status and the expression of proangiogenic factors. (A) Representative flow cytometry histogram showing the fluorescence intensity of 6 macrophage polarization markers. (B) Bar charts showing the MFIs of macrophage polarization markers ( n = 3; one‐way ANOVA and Tukey's multiple comparisons test). (C) Representative western blot showing the relative levels of iNOS, TNF‐α, IL‐6, VEGFA, IGF1, EGR1, IL‐10, and MMP9 (normalized to β‐Actin expression) in primary macrophages. (D) Densitometric analyses of the relative levels of iNOS, TNF‐α, IL‐6, VEGFA, IGF1, EGR1, IL‐10, and MMP9 ( n = 3; one‐way ANOVA and Tukey's multiple comparisons test). (E) qPCR results showing the relative mRNA expression of iNOS , TNF‐α , IL‐6 , VEGFA , IGF1 , EGR1 , IL‐10 , and MMP9 in primary macrophages ( n = 3; one‐way ANOVA and Tukey's multiple comparisons test). (F) Representative flow cytometry histogram showing the fluorescence intensity of macrophage polarization markers. (G) Bar charts showing the MFIs of macrophage polarization markers ( n = 3; one‐way ANOVA and Tukey's multiple comparisons test). (H) Representative western blot showing the relative levels of iNOS, TNF‐α, IL‐6, VEGFA, IGF1, EGR1, IL‐10, and MMP9 (normalized to β‐Actin expression) in TDMs. (I) Densitometric analyses of the relative levels of iNOS, TNF‐α, IL‐6, VEGFA, IGF1, EGR1, IL‐10, and MMP9 ( n = 3; one‐way ANOVA and Tukey's multiple comparisons test). (J) qPCR results showing the relative mRNA expression of iNOS , TNF‐α , IL‐6 , VEGFA , IGF1 , EGR1 , IL‐10 , and MMP9 in TDMs ( n = 3; one‐way ANOVA and Tukey's multiple comparisons test). The error bars represent the ± SDs. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. MFI, mean fluorescence intensity; TDMs, THP‐1‐derived macrophages; TEMs, Tie2‐expressing monocytes/macrophages; TNMs, Tie2‐negative monocytes/macrophages.
Article Snippet: In accordance with previous studies,
Techniques: Flow Cytometry, Western Blot, Expressing, Fluorescence, Derivative Assay
Journal: CNS Neuroscience & Therapeutics
Article Title: ANGPT2 /Tie2 Enhances H3K18la ‐Mediated Macrophage M2 Polarization to Promote Endothelial Cell Proliferation in the Chronically Ischaemic Brain
doi: 10.1002/cns.70879
Figure Lengend Snippet: Western blot and immunofluorescence staining showing the expression of relevant cytokines in the CIB of 2VO + EMS rats. (A) Representative images of triple immunofluorescence staining showing Tie2 + , CD11b + , and CD206 + cells in the CIB of each group. Bar = 50 μm. (B) Counts of M2 TEMs (Tie2 + /CD11b + /CD206 + ) in each group ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (C) Representative western blot showing the relative expression of ANGPT2, CD206, and ARG1 (normalized to β‐Actin expression). (D) Densitometric analyses of the relative expression of ANGPT2, CD206, and ARG1 ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (E) Representative images of double immunofluorescence staining showing CD31 + and Ki67 + cells in the CIB of each group. Bar = 50 μm. (F) Column chart showing the counts of CD31 + cells in each group ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (G) Column chart showing the proportion of Ki67‐positive ECs in each group ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (H) Representative western blot showing the relative expression of CD31 (normalized to β‐Actin expression). (I) Densitometric analyses of the relative expression of CD31 ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (H) * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. The error bars represent the ±SDs. CIB: Chronically ischaemic brain; TEMs, Tie2‐expressing monocytes/macrophages.
Article Snippet: In accordance with previous studies,
Techniques: Western Blot, Immunofluorescence, Staining, Expressing, Double Immunofluorescence Staining
Journal: CNS Neuroscience & Therapeutics
Article Title: ANGPT2 /Tie2 Enhances H3K18la ‐Mediated Macrophage M2 Polarization to Promote Endothelial Cell Proliferation in the Chronically Ischaemic Brain
doi: 10.1002/cns.70879
Figure Lengend Snippet: Behavioral test, western blotting, and immunofluorescence results in 2VO + EMS rats 4‐week post‐modeling. (A) Quantitative analysis of the percentage of recognition preference for the novel object in each group rats in NORT test ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (B) Representative image showing the swimming paths in each group rats during the MWM test. (C) Line chart showing the average escape latencies in each group rats during the MWM test ( n = 6; two‐way ANOVA and Tukey's multiple comparisons test; ***: TEMs vs. TEMs ANGPT2 , ****: TEMs ANGPT2 vs. TEMs ANGPT2+Oxamate , TEMs ANGPT2 vs. TEMs ANGPT2+C646 , TEMs ANGPT2 vs. TEMs ANGPT2+LPS ). (D) Quantitative analysis of the time spent in target quadrant in each group rats during the MWM test ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (E) Quantitative analysis of the number of platform crossings in each group rats during the MWM test ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (F) Quantitative analysis of the average swimming speed in each group rats during the MWM test ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (G) Representative western blot showing the relative expression of CD31 (normalized to β‐Actin expression). (H) Densitometric analyses of the relative expression of CD31 ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (I) Representative images of double immunofluorescence staining showing CD31 + and Ki67 + cells in the CIB of each group. Bar = 50 μm. (J) Column chart showing the counts of CD31 + cells in each group ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). (K) Column chart showing the proportion of Ki67‐positive ECs in each group ( n = 6; one‐way ANOVA and Tukey's multiple comparisons test). The error bars represent the ± SDs. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. 2VO, 2‐vessel occlusion; EMS, encephalomyosynangiosis; MWM, Morris water maze; NORT, novel object recognition test; TEMs, Tie2‐expressing monocytes/macrophages.
Article Snippet: In accordance with previous studies,
Techniques: Western Blot, Immunofluorescence, Expressing, Double Immunofluorescence Staining