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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Biallelic Mutations in ACACA Cause a Disruption in Lipid Homeostasis That Is Associated With Global Developmental Delay, Microcephaly, and Dysmorphic Facial Features
doi: 10.3389/fcell.2021.618492
Figure Lengend Snippet: Summary of the genetic characteristics and SDS-PAGE of ACC1 protein content and ACC1 enzyme activity. (A) The pedigree of the affected individual. The proband was represented as black solid circle and pointed out by an arrow. The square indicates male, and the circle female. (B) Sanger sequencing of the mutations. (C) The amino acid conservation among different species. (D) The functional domain of ACC1, and the mutation sites were marked. (E) Relative ACC1 protein content of lymphocytes. (F) The ACC1 enzyme activity among the proband- and age-matched control-derived lymphocytes. One-way ANOVA applied in panels (E,F) . ** p < 0.01.
Article Snippet: According to the
Techniques: SDS Page, Activity Assay, Sequencing, Functional Assay, Mutagenesis, Control, Derivative Assay
Journal: Frontiers in Cell and Developmental Biology
Article Title: Biallelic Mutations in ACACA Cause a Disruption in Lipid Homeostasis That Is Associated With Global Developmental Delay, Microcephaly, and Dysmorphic Facial Features
doi: 10.3389/fcell.2021.618492
Figure Lengend Snippet: ACC1-knockdown (KD) fibroblasts and the cell motility capacity measurement. (A) The ACC1 protein content of ACC1-KD fibroblast cell lines, and (B) the ACC1 enzyme activity. The wound healing assay was carried out at different time points (0, 12, and 24 h) (C) without or (E) with palmitate (scale bar = 30 μm), and (D,F) show quantitative analysis, respectively. The Trans-well migration capacity at 100× magnification (upper) and 200× magnification (lower) (G) with or (I) without palmitate, respectively. (H,J) The quantitative analyses, respectively. All data were from three independent replicates and analyzed by mean ± SEM. Independent t -test in panels (A,B,D,F,H,J) . * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: According to the
Techniques: Knockdown, Activity Assay, Wound Healing Assay, Migration