Journal: International Journal of Molecular Sciences

Article Title: The Novel HSF1 Inhibitor NXP800 Exhibits Robust Antitumor Activity in Hepatocellular Carcinoma

doi: 10.3390/ijms27062781

Figure Lengend Snippet: Cytotoxicity assessment of the HSF1 inhibitor NXP800 in human hepatocellular carcinoma (HCC) cell lines. ( A ) The levels of the HSF1 protein were assessed in Hep3B, HLE, HLF, Huh7, and SNU449 cell lines by Western blot analysis. GAPDH was used as a loading control. ( B ) Cell viability was measured via MTT assay. The cell viability in percentage to DMSO control, expressed as non-linear regression curve fit over log10 drug concentrations, is depicted. The mean ± SD (n = 3) of at least three independent experiments each performed in triplicate is shown. Horizontal dotted line: 50% viable cells. IC 50 values per cell line are shown in each plot.

Article Snippet: The human hepatocellular carcinoma cell lines HLE (XenoTech, Kansas City, KS, USA; Cat. No. JCRB0404), HLF (Tebubio GmbH, Le Perray en Yvelines, France; Cat. No. JCRB0405), PLC/PRF/5 (CLS Cell Lines Service GmbH, Eppelheim, Germany; Cat. No. 300315), Huh7 (Cytion GmbH, Heidelberg, Germany; Cat. No. 300156), and SNU449 (LGC Standards GmbH, Teddington, Middlesex, UK; Cat. No. CRL-2234) were purchased as indicated and cultured according to standard procedures.

Techniques: Western Blot, Control, MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: FGL1 expression level and viability in HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Expression of FGL1 in the six HCC cell lines, as determined through immunoblotting. Data are presented as the mean ± standard deviation (SD) (n = 3). *** p < 0.001, significantly different from HCC cells with high FGL1-expressing. ( B ) Cell viability in the six HCC cell lines after sorafenib treatment. Data are presented as the mean ± standard deviation (n = 6).

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: IC 50 of sorafenib against HCC cell lines.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of sorafenib on the cell colony forming capacity and cell death of HepG2, Huh7, Hep3B, SNU387, SNU449, and SNU475 hepatocellular carcinoma (HCC) cell lines. ( A ) Number of colonies after seeding 200 cells of each HCC cell line in the plate. ( B ) Effect of sorafenib on the colony forming potential of HCC cells. The six HCC cell lines were incubated for 7 days in the presence of 0, 2, or 5 µM sorafenib. Data are presented as the mean ± standard deviation (SD) (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from 0 µM sorafenib-treated cells. ( C ): Effect of sorafenib on cell death measured by flow cytometry using Annexin V-FITC and propidium iodide (PI) staining. The graph presents mean ± SD values of three independent experiments.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Incubation, Standard Deviation, Flow Cytometry, Staining

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of sorafenib on cell death and cell proliferation factors in high FGL1- and low FGL1-expressing hepatocellular carcinoma (HCC) cell lines. ( A ): Protein expression was analyzed by western blotting 48 h after treatment with 10 μM sorafenib. Expression levels of PCNA, p-ERK/ERK, LC3-II, and cleaved PARP1 quantified in Huh7 ( B ), Hep3B ( C ), SNU387 ( D ), and SNU475 ( E ) cell lines. Data are presented as the mean ± standard deviation (SD) (n = 3). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from 0 µM sorafenib-treated cells.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of silencing FGL1 in Huh7 and Hep3B cells on their sorafenib sensitivity. ( A ) Expression of FGL1 in Huh7 cells 24 h after FGL1 siRNA transfection. ( B ) Colony formation of Huh7 cells following siFGL1 and/or sorafenib (2 µM) treatment. ( C ) Expression of FGL1 in Hep3B cells 24 h after FGL1 siRNA transfection. ( D ) Colony formation of Hep3B cells following siFGL1 and/or sorafenib (2 µM) treatment. Graphs present mean ± standard deviation values (n = 4). * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from scrambled siRNA-treated cells. siFGL1 (−), treated with siScr; Sorafenib (−), treated with vehicle (DMSO); siScr, scrambled siRNA; siFGL1, FGL1 siRNA.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Expressing, Transfection, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of FGL1 knockdown on sorafenib-induced cell death in Huh7 and Hep3B cell lines. Flow cytometry results of Huh7 ( A , B ) and Hep3B ( C , D ) cells are shown. The cell death percentage was estimated using the sum of the upper left, upper right, and lower right quadrants in each dot plot. The graphs present percentage of cell death. All values are presented as the mean ± standard deviation of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001, significantly different from scrambled siRNA-treated cells; # p < 0.05, significantly different from FGL1 siRNA-treated cells. siFGL1 (−), treated with siScr; Sorafenib (−), treated with vehicle (DMSO); siScr, scrambled siRNA; siFGL1, FGL1 siRNA.

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Knockdown, Flow Cytometry, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells

doi: 10.3390/ijms22105330

Figure Lengend Snippet: Effect of FGL1 knockdown on p-ERK, ERK, Beclin-1, and LC3-II in hepatocellular carcinoma (HCC) cells following sorafenib treatment. Representative western blot and data from Huh7 ( A ) and Hep3B ( B ) cells. Data are presented as the mean ± standard deviation (n = 3). * p < 0.05 and ** p < 0.01, compared to siScr-treated cells. siFGL1 (−), treated with siScr. Sorafenib (−), treated with vehicle (DMSO).

Article Snippet: The cell lines and their culture media were as follows: HepG2 (ATCC, Manassas, VA, USA), DMEM; Huh7 (KCLB, Korean Cell Line Bank, Seoul, Korea), RPMI1640; Hep3B (KCLB), DMEM; SNU387 (KCLB), RPMI1640; SNU475 (KCLB), RPMI1640; SNU 449 (KCLB), RPMI1640.

Techniques: Knockdown, Western Blot, Standard Deviation

Journal: Antiviral research

Article Title: Feasibility and biological rationale of repurposing sunitinib and erlotinib for dengue treatment

doi: 10.1016/j.antiviral.2018.05.001

Figure Lengend Snippet: AAK1 and GAK transcript levels in bulk (A) and single cell (B-C) DENV infected (MOI=0 (control), 1, 10) Huh7 samples at various time points postinfection, measured via qRT-PCR (A) and virus-inclusive single-cell RNA-Seq (B-C). *P < 0.05, **P < 0.01 in A are relative to vehicle control normalized to TBP (TATA-binding protein) by two-way ANOVA with Tukey’s multiple comparisons test. B. Scatter plots depicting the correlation between vRNA content (viral reads, X axis) and AAK1/GAK gene expression (Y axis) during DENV infection. Each dot represents a single cell, color-coded based on the MOI. ρ represents Spearman correlation coefficients between abundances of dengue vRNA and of AAK1/GAK mRNA from the same cell. C. Correlation values measured for AAK1 (green) and GAK (orange) at various time points postinfection.

Article Snippet: Huh7 (Apath LLC), BHK-21 (ATCC), and T-REx 293 (ThermoFisher) cells were grown in DMEM (Mediatech) supplemented with 10% FBS (Omega Scientific), nonessential amino acids, 1% L -glutamine, and 1% penicillin-streptomycin (ThermoFisher Scientific) (5% CO 2 , 37°C).

Techniques: Infection, Control, Quantitative RT-PCR, Virus, RNA Sequencing, Binding Assay, Gene Expression