Journal: Oncotarget

Article Title: Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of urothelial cancer and normal urothelial cells

doi: 10.18632/oncotarget.21995

Figure Lengend Snippet: (A) Schematic representation of experimental conditions including non-irradiated, uniformly-irradiated and shielding protocols where 50% of the slide was exposed and 50% shielded with MCP alloy. Cells were fixed and processed for 53BP1-immunofluorescence, one hour following irradiation. Micrographs of urothelial cancer T24 cells labelled with anti-53BP1 (green) to label nuclear DNA double stand break foci and DAPI to counterstain nuclei are shown for each experimental condition. (B) Summary graph of mean foci/cell in T24 cells (N=3), measured from confocal z-stacks in Volocity software. There were significantly more foci in shielded cells 0.5mm from the shield than in non-irradiated controls, indicative of a bystander effect (p=0.019). (C) Summary data for HT1376 cancer cells (N=3) where there was no difference in the mean foci/cell between non-irradiated controls and shielded cells. (D) Summary data for normal urothelial HUC (N=3). Mean foci/cell was significantly greater in shielded cells in the 0-5mm (p=0.005) and 5-10mm (p=0.04) analysis regions from the shield. * denotes p<0.05, ** denotes p<0.01

Article Snippet: Urothelial carcinoma cells T24 (McCoy’s 5A) [ ], HT1376 MEM) [ ], immortalised normal urothelial cells SV-HUC (F12K) [ ], were purchased from ATCC; primary normal HUC (Urothelial Cell Medium) were obtained from ScienCell Research Laboratories.

Techniques: Irradiation, Immunofluorescence, Software

Journal: Oncotarget

Article Title: Dual effects of radiation bystander signaling in urothelial cancer: purinergic-activation of apoptosis attenuates survival of urothelial cancer and normal urothelial cells

doi: 10.18632/oncotarget.21995

Figure Lengend Snippet: Summary schematic diagram of the radiation-bystander effect that is common to urothelial cancer (T24) cells and normal urothelium (SV-HUC). Directly irradiated cells signal to bystander cells activating pro-apoptotic signaling mechanisms and causing DNA double stand break damage resulting in decreased cell survival. The bystander effect studied here was mediated by ATP release from irradiated cells that acted on bystander cells.

Article Snippet: Urothelial carcinoma cells T24 (McCoy’s 5A) [ ], HT1376 MEM) [ ], immortalised normal urothelial cells SV-HUC (F12K) [ ], were purchased from ATCC; primary normal HUC (Urothelial Cell Medium) were obtained from ScienCell Research Laboratories.

Techniques: Irradiation

Journal: BMC Complementary and Alternative Medicine

Article Title: The investigation of a traditional Chinese medicine, Guizhi Fuling Wan (GFW) as an intravesical therapeutic agent for urothelial carcinoma of the bladder

doi: 10.1186/1472-6882-13-44

Figure Lengend Snippet: Cytotoxicity of GFW and Cinnamomi Ramulus against normal human urothelial cell line, HUC 4449: Cells were initially seeded in 96-well plates at 1 × 10 4 cells per well and cultured for 24 h. The cells were subsequently starved in medium supplemented without FBS for 24 h, and then treated with various concentrations of agents for 24 h. Cell viability was detected using the Cell Counting Kit-8 (CCK-8). Data are presented as mean ± SEM (n=6). Significant differences from the no treatment control is indicated by * * (p<0.01), as determined by one-way ANOVA and Dunnett’s comparison test.

Article Snippet: Human bladder papillary transitional cells, BFTC 905, and bladder carcinoma cells, TSGH 8301 (Food Industry Research and Development Institute, Taiwan, ROC) as well as primary normal urothelial cells, HUC 4449 (ScienCell Research Laboratories, Carlsbad, CA, USA) were used as cell models.

Techniques: Cell Culture, Cell Counting, CCK-8 Assay, Control, Comparison

Journal: BMC Complementary and Alternative Medicine

Article Title: The investigation of a traditional Chinese medicine, Guizhi Fuling Wan (GFW) as an intravesical therapeutic agent for urothelial carcinoma of the bladder

doi: 10.1186/1472-6882-13-44

Figure Lengend Snippet: Cytotoxicity of GFW, cisplatin, Epirubicin and mitomycin against normal human urothelial cell line, HUC 4449 and bladder cancer cell lines, TSGH 8301 and BFTC 905: Cells were initially seeded in 96-well plates at 1 × 10 4 cells per well and cultured for 24 h. The cells were subsequently starved in medium supplemented without FBS for 24 h and then treated with various concentrations of agents for 24 h. Cell viability was detected using the Cell Counting Kit-8 (CCK-8). Data are presented as mean ±SEM (n=6). Significant differences from the no treatment control is indicated by * * (p<0.01), as determined by one-way ANOVA and Dunnett’s comparison test.

Article Snippet: Human bladder papillary transitional cells, BFTC 905, and bladder carcinoma cells, TSGH 8301 (Food Industry Research and Development Institute, Taiwan, ROC) as well as primary normal urothelial cells, HUC 4449 (ScienCell Research Laboratories, Carlsbad, CA, USA) were used as cell models.

Techniques: Cell Culture, Cell Counting, CCK-8 Assay, Control, Comparison