htlr4 cells Search Results


inflammasome activators  (Thermo Fisher)
96
Thermo Fisher inflammasome activators
Lidocaine in combination with dexamethasone has an additive modulatory effect on the innate inflammatory pathway of <t>inflammasome</t> activation. THP1-ASC-GFP cells were primed with EcLPS for 3 h, stimulated ON with transfected pcDNA and modulated or not with lidocaine and/or dexamethasone. Cells were prepared for fluorescent microscopy observation (A, 20×), intense green specks and cells were counted and ASC + cells/total cells ratio calculated (B) and hIL-1β was measured in culture supernatants (C). ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗ ( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), ### ( p < 0.001), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.
Inflammasome Activators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
inflammasome activators - by Bioz Stars, 2026-04
96/100 stars
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hek-blue htlr4 cells  (InvivoGen)
96
InvivoGen hek-blue htlr4 cells
Lidocaine in combination with dexamethasone has an additive modulatory effect on the innate inflammatory pathway of <t>inflammasome</t> activation. THP1-ASC-GFP cells were primed with EcLPS for 3 h, stimulated ON with transfected pcDNA and modulated or not with lidocaine and/or dexamethasone. Cells were prepared for fluorescent microscopy observation (A, 20×), intense green specks and cells were counted and ASC + cells/total cells ratio calculated (B) and hIL-1β was measured in culture supernatants (C). ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗ ( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), ### ( p < 0.001), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.
Hek Blue Htlr4 Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek-blue htlr4 cells/product/InvivoGen
Average 96 stars, based on 1 article reviews
hek-blue htlr4 cells - by Bioz Stars, 2026-04
96/100 stars
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htlr4 conjugated phycoerythrin (pe  (Becton Dickinson)
90
Becton Dickinson htlr4 conjugated phycoerythrin (pe
Lidocaine in combination with dexamethasone has an additive modulatory effect on the innate inflammatory pathway of <t>inflammasome</t> activation. THP1-ASC-GFP cells were primed with EcLPS for 3 h, stimulated ON with transfected pcDNA and modulated or not with lidocaine and/or dexamethasone. Cells were prepared for fluorescent microscopy observation (A, 20×), intense green specks and cells were counted and ASC + cells/total cells ratio calculated (B) and hIL-1β was measured in culture supernatants (C). ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗ ( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), ### ( p < 0.001), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.
Htlr4 Conjugated Phycoerythrin (Pe, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/htlr4 conjugated phycoerythrin (pe/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
htlr4 conjugated phycoerythrin (pe - by Bioz Stars, 2026-04
90/100 stars
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hek293-htlr4-gfp stable cell lines  (Aurogene Srl)
90
Aurogene Srl hek293-htlr4-gfp stable cell lines
Lidocaine in combination with dexamethasone has an additive modulatory effect on the innate inflammatory pathway of <t>inflammasome</t> activation. THP1-ASC-GFP cells were primed with EcLPS for 3 h, stimulated ON with transfected pcDNA and modulated or not with lidocaine and/or dexamethasone. Cells were prepared for fluorescent microscopy observation (A, 20×), intense green specks and cells were counted and ASC + cells/total cells ratio calculated (B) and hIL-1β was measured in culture supernatants (C). ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗ ( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), ### ( p < 0.001), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.
Hek293 Htlr4 Gfp Stable Cell Lines, supplied by Aurogene Srl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek293-htlr4-gfp stable cell lines/product/Aurogene Srl
Average 90 stars, based on 1 article reviews
hek293-htlr4-gfp stable cell lines - by Bioz Stars, 2026-04
90/100 stars
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hek-bluetm htlr4 cells  (Vivogen Biotechnology Inc)
90
Vivogen Biotechnology Inc hek-bluetm htlr4 cells
Macrophage activation by violacein-killed tumor cell supernatant. HCT116, Huh7, and PANC-1 cells were treated with 50 µM violacein or oxaliplatin for 4 h before the media was changed. Then, 24 h after treatment, supernatants from untreated (co) and compound-treated cells were collected, and dead cells were removed. (A-C) RAW-Blue™, (D-F) THP1-XBlue™, (G, H) HEK-Blue™ <t>hTLR4,</t> and (J-L) HEK-Blue™ Null2 reporter cells were incubated with the respective dead tumor cell-conditioned medium (dTCM, 50% v/v) or LPS (100 ng/ml for RAW-Blue™ and 10 ng/ml for THP1X-Blue™, HEK-Blue™ hTLR4, and HEK-Blue™ Null2) for 24 h. The activation of the reporter cells was determined by colorimetric QUANTI-Blue™.
Hek Bluetm Htlr4 Cells, supplied by Vivogen Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hek-bluetm htlr4 cells/product/Vivogen Biotechnology Inc
Average 90 stars, based on 1 article reviews
hek-bluetm htlr4 cells - by Bioz Stars, 2026-04
90/100 stars
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puromycin  (InvivoGen)
99
InvivoGen puromycin
Macrophage activation by violacein-killed tumor cell supernatant. HCT116, Huh7, and PANC-1 cells were treated with 50 µM violacein or oxaliplatin for 4 h before the media was changed. Then, 24 h after treatment, supernatants from untreated (co) and compound-treated cells were collected, and dead cells were removed. (A-C) RAW-Blue™, (D-F) THP1-XBlue™, (G, H) HEK-Blue™ <t>hTLR4,</t> and (J-L) HEK-Blue™ Null2 reporter cells were incubated with the respective dead tumor cell-conditioned medium (dTCM, 50% v/v) or LPS (100 ng/ml for RAW-Blue™ and 10 ng/ml for THP1X-Blue™, HEK-Blue™ hTLR4, and HEK-Blue™ Null2) for 24 h. The activation of the reporter cells was determined by colorimetric QUANTI-Blue™.
Puromycin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/puromycin/product/InvivoGen
Average 99 stars, based on 1 article reviews
puromycin - by Bioz Stars, 2026-04
99/100 stars
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Image Search Results


Lidocaine in combination with dexamethasone has an additive modulatory effect on the innate inflammatory pathway of inflammasome activation. THP1-ASC-GFP cells were primed with EcLPS for 3 h, stimulated ON with transfected pcDNA and modulated or not with lidocaine and/or dexamethasone. Cells were prepared for fluorescent microscopy observation (A, 20×), intense green specks and cells were counted and ASC + cells/total cells ratio calculated (B) and hIL-1β was measured in culture supernatants (C). ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗ ( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), ### ( p < 0.001), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.

Journal: Biomedical Journal

Article Title: Lidocaine reinforces the anti-inflammatory action of dexamethasone on myeloid and epithelial cells activated by inflammatory cytokines or SARS-CoV-2 infection

doi: 10.1016/j.bj.2022.07.008

Figure Lengend Snippet: Lidocaine in combination with dexamethasone has an additive modulatory effect on the innate inflammatory pathway of inflammasome activation. THP1-ASC-GFP cells were primed with EcLPS for 3 h, stimulated ON with transfected pcDNA and modulated or not with lidocaine and/or dexamethasone. Cells were prepared for fluorescent microscopy observation (A, 20×), intense green specks and cells were counted and ASC + cells/total cells ratio calculated (B) and hIL-1β was measured in culture supernatants (C). ∗ ( p < 0.05), ∗∗ ( p < 0.01), ∗∗∗ ( p < 0.001), ∗∗∗∗ ( p < 0.0001), a result significantly different from the stimulated unmodulated condition. # ( p < 0.05), ## ( p < 0.01), ### ( p < 0.001), a result significantly different from its counterpart stimulated and modulated with only lidocaine at the same concentration. Data are represented as mean ± SEM of three replicates. Graphs are representative of at least two independent assays.

Article Snippet: Inflammasome activators: Ultra-pure EcLPS (InvitroGen®), transfected pcDNA (generated by PCR cloning of a cDNA fragment of a random not related gene) with LipofectamineTM LTX Reagent with PLUSTM Reagent (Invitrogen®) following the manufacturer instructions.

Techniques: Activation Assay, Transfection, Microscopy, Concentration Assay

Macrophage activation by violacein-killed tumor cell supernatant. HCT116, Huh7, and PANC-1 cells were treated with 50 µM violacein or oxaliplatin for 4 h before the media was changed. Then, 24 h after treatment, supernatants from untreated (co) and compound-treated cells were collected, and dead cells were removed. (A-C) RAW-Blue™, (D-F) THP1-XBlue™, (G, H) HEK-Blue™ hTLR4, and (J-L) HEK-Blue™ Null2 reporter cells were incubated with the respective dead tumor cell-conditioned medium (dTCM, 50% v/v) or LPS (100 ng/ml for RAW-Blue™ and 10 ng/ml for THP1X-Blue™, HEK-Blue™ hTLR4, and HEK-Blue™ Null2) for 24 h. The activation of the reporter cells was determined by colorimetric QUANTI-Blue™.

Journal: Frontiers in Oncology

Article Title: Characterization of Anti-Cancer Activities of Violacein: Actions on Tumor Cells and the Tumor Microenvironment

doi: 10.3389/fonc.2022.872223

Figure Lengend Snippet: Macrophage activation by violacein-killed tumor cell supernatant. HCT116, Huh7, and PANC-1 cells were treated with 50 µM violacein or oxaliplatin for 4 h before the media was changed. Then, 24 h after treatment, supernatants from untreated (co) and compound-treated cells were collected, and dead cells were removed. (A-C) RAW-Blue™, (D-F) THP1-XBlue™, (G, H) HEK-Blue™ hTLR4, and (J-L) HEK-Blue™ Null2 reporter cells were incubated with the respective dead tumor cell-conditioned medium (dTCM, 50% v/v) or LPS (100 ng/ml for RAW-Blue™ and 10 ng/ml for THP1X-Blue™, HEK-Blue™ hTLR4, and HEK-Blue™ Null2) for 24 h. The activation of the reporter cells was determined by colorimetric QUANTI-Blue™.

Article Snippet: HEK-BlueTM hTLR4 cells and HEK-BlueTM Null2 cells (In vivoGen) were grown in DMEM supplemented with 10% heat-inactivated FCS (30 min at 56°C), 100 U/ml penicillin/streptomycin, 2 mM glutamine, 100 μg/ml Normocin, and 1x HEK-BlueTM Selection.

Techniques: Activation Assay, Incubation