Journal: Journal of Translational Medicine

Article Title: Formyl peptide receptor 2 activation by MR-39 inhibits glioblastoma cell proliferation and invasiveness through suppression of multiple oncogenic pathways

doi: 10.1186/s12967-026-07781-3

Figure Lengend Snippet: Antiproliferative effect of MR-39 on human glioblastoma cell lines. ( A ) U87-MG, U138-MG, and U251-MG cells were exposed to 5-100 µM of MR-39 for 48 h. ( B ) Number of U87-MG, U138MG, and U251-MG cells exposed for 48 h to either the vehicle (CTRL) or 10 µM MR-39. ( C ) U87-MG, U138-MG, and U251-MG cells were exposed to either vehicle (CTRL) or 10 µM MR-39, with or without the FPR2 antagonist WRW4 (10 µM). ( D ) U87-MG cells were transfected with FPR2-targeting antisense siRNA1 or a negative control (siRNA-), followed by treatment with or without 10 µM MR-39. Cell proliferation was measured using the CCK-8 assay and is expressed as a percentage of the control. Data are expressed as means ± SEM, n = 3. Statistically significant differences are based on an unpaired t-test * p value < 0.05, ** p value < 0.005, *** p value < 0.001, **** p value < 0.0001 or one-way ANOVA followed by Tukey’s multiple comparison test

Article Snippet: EA.hy926 (CRL-2922 TM) andU87-MG (HTB-14 TM) cell lines were acquired from ATCC, while U138MG, and U251-MG were kindly provided by Prof. Generoso Luca Colucci D’Amato, University of Campania “Luigi Vanvitelli” [ ].

Techniques: Transfection, Negative Control, CCK-8 Assay, Control, Comparison

Journal: Nature Communications

Article Title: Supercharging-enhanced nDIA-MS enables global profiling of drug-induced proteome solubility shifts

doi: 10.1038/s41467-026-69025-8

Figure Lengend Snippet: Volcano plots showing the comparisons of protein levels between MG132 vs vehicle, and MG132 + ML-792 vs ML-792 from ( A ) U2OS, ( B ) HeLa, and ( C ) HEK293A cells. Volcano plots showing the comparisons of protein levels between ML-792 vs vehicle, and MG132 + ML-792 vs MG132 from ( D ) U2OS, ( E ) HeLa, and ( F ) HEK293A cells. P -values were calculated using a two-tailed Student’s t-test. No adjustments for multiple comparisons were applied. G Venn diagram showing the overlaps of up- and down-regulated proteins obtained from three cancer cell lines following treatment of MG132. Differentially regulated proteins from two pairwise comparisons of MG132 vs vehicle and MG132 + ML-792 vs ML-792 were combined. H Venn diagram showing the overlaps of up- and down-regulated proteins obtained from three cancer cell lines following treatment of ML-792. I , J Heatmaps showing differential proteins modulated by MG132 ( I ) and ML792 ( J ), respectively. Only proteins dysregulated in at least two cell lines are displayed. Western blotting results of representative proteins modulated by MG132 or ML792, including C-FOS ( K ), EGR1 ( K ), CCN1/2 ( L ), HIF1A ( M ). Experiments were repeated at least three times, and similar results were obtained. Source data are provided as a Source Data file.

Article Snippet: U2OS cells were purchased from the ATCC (Manassas, VA) and maintained in McCoy’s 5 A (Modified) medium containing 10% fetal calf serum at 37 °C with 5% CO 2 .

Techniques: Two Tailed Test, Western Blot

Journal: Nature Communications

Article Title: Supercharging-enhanced nDIA-MS enables global profiling of drug-induced proteome solubility shifts

doi: 10.1038/s41467-026-69025-8

Figure Lengend Snippet: Volcano plots showing the comparisons of relative insolubility rates between MG132 vs vehicle, and MG132 + ML-792 vs ML-792 from ( A ) U2OS, ( B ) HeLa, and ( C ) HEK293A cells. P -values were calculated using a two-tailed Student’s t-test. No adjustments for multiple comparisons were applied. Venn diagram showing the overlaps of ( D ) up- and ( E ) down-regulated proteins obtained from three cancer cell lines. Differentially regulated proteins from two pairwise comparisons of MG132 vs vehicle and MG132 + ML-792 vs ML-792 were combined. Functional enrichment analysis of the proteins whose insolubility rates were consistently ( F ) up- and ( G ) down-regulated in at least two cell lines. Enrichment analysis was performed using DAVID with default settings. P-values were calculated using the modified Fisher’s exact test (EASE score). No adjustments for multiple comparisons were applied. H–J Heatmaps showing proteins modulated by MG132. K–M Western blotting result of representative proteins that modulated by MG132 or ML792, including BAG6, UBL4A, TEX264, HSF1, REV1, and ATR1. Experiments were repeated at least three times, and similar results were obtained. Source data are provided as a Source Data file.

Article Snippet: U2OS cells were purchased from the ATCC (Manassas, VA) and maintained in McCoy’s 5 A (Modified) medium containing 10% fetal calf serum at 37 °C with 5% CO 2 .

Techniques: Two Tailed Test, Functional Assay, Modification, Western Blot

Journal: Nature Communications

Article Title: Supercharging-enhanced nDIA-MS enables global profiling of drug-induced proteome solubility shifts

doi: 10.1038/s41467-026-69025-8

Figure Lengend Snippet: Volcano plots showing the comparisons of relative insolubility rates between ML-792 vs vehicle, and MG132 + ML-792 vs MG132 from ( A ) U2OS, ( B ) HeLa, and ( C ) HEK293A cells. P -values were calculated using a two-tailed Student’s t-test. No adjustments for multiple comparisons were applied. Venn diagram showing the overlaps of ( D ) up- and ( E ) down-regulated proteins obtained from three cancer cell lines. Differentially regulated proteins from two pairwise comparisons of ML-792 vs vehicle and MG132 + ML-792 vs MG132 were combined. F Functional enrichment analysis of the proteins whose insolubility rates were consistently up- and down-regulated in at least two cell lines. Enrichment analysis was performed using DAVID with default settings. P-values were calculated using the modified Fisher’s exact test (EASE score). No adjustments for multiple comparisons were applied. G–I Heatmaps showing proteins modulated by ML-792. J–L Western blotting result of representative proteins that modulated by MG132 or ML792, including FANCD2, POLR3G, SP100, and DAXX. Experiments were repeated at least three times, and similar results were obtained. Source data are provided as a Source Data file.

Article Snippet: U2OS cells were purchased from the ATCC (Manassas, VA) and maintained in McCoy’s 5 A (Modified) medium containing 10% fetal calf serum at 37 °C with 5% CO 2 .

Techniques: Two Tailed Test, Functional Assay, Modification, Western Blot