Journal: Virologica Sinica

Article Title: Nuclear translocation of Sp100A suppresses multiple DNA viruses and limits HSV-1 lytic replication in vivo

doi: 10.1016/j.virs.2026.02.003

Figure Lengend Snippet: Modulation of viral replication by Sp100A mutations on HSV-1. A HEp-2 cells were mock infected, or with the indicated viruses at an MOI of 5 for 6 h. Protein expression was evaluated by immunoblotting with anti-Flag, anti-β-actin, anti-TK, anti-ICP0, and anti-ICP8 antibodies. Note that the target proteins (Flag, TK, ICP0, and ICP8) were detected on separate blots. β-actin was used as a loading control and was run on a parallel blot using the same batch of protein lysates and identical loading amounts to confirm equal sample loading. B, C Growth kinetics of the indicated viruses in HEp-2 cells at MOIs of 0.01 ( B ) and 5 ( C ). D, E Growth kinetics of the indicated viruses in Vero cells at MOIs of 0.01 ( D ) and 5 ( E ). Data in B – E , statistical comparisons of viral titers were performed between group rHSV-1 and groups rHSV-1-A, rHSV-1-A S188A and rHSV-1-A S188D , as well as between group rHSV-1-A S188A and groups rHSV-1-A and rHSV-1-A S188D . Statistical analyses were performed using two-way ANOVA. F Plaque size comparison of the indicated viruses in HEp-2 and Vero cells. G, H Plaque size of each virus was quantified from 50 plaques using ImageJ and plotted. The data were normalized to the mean plaque size of the rHSV-1-A S188A group. Statistical analyses were performed using one-way ANOVA. Data presented as mean ± SD. ∗∗∗∗P < 0.0001; ns, not significant.

Article Snippet: Antibodies and drugs used in this study included the following: rabbit polyclonal anti-Flag antibody (Catalog No. 80010, Proteintech, USA), rabbit polyclonal anti-Sp100 antibody (Catalog No. GTX131569, GeneTex, USA), mouse monoclonal anti-Flag antibody (Catalog No. AB0008; Abways, Beijing, China), mouse monoclonal anti-β-actin antibody (Catalog No. 1000166; Sino Biological, Beijing, China), rabbit monoclonal anti-GAPDH antibody (Catalog No. AB0037, Abways Technology, Beijing, China), mouse monoclonal anti-Histone H3 antibody (Catalog No. 100005-MM01, Sino Biological, Beijing, China), rabbit polyclonal anti-TSG101 antibody (Catalog No. 28283-1-AP; Proteintech; USA), mouse monoclonal anti-calnexin antibody (Catalog No. sc23954, Santa Cruz, USA), mouse monoclonal anti-ICP0 antibody (Catalog No. sc53070, Santa Cruz, USA), mouse monoclonal anti-ICP8 antibody (Catalog No. ab20194, Abcam, UK), rabbit polyclonal anti-TK antibody (custom-made, GenicBio Limited, Shanghai, China), Alexa Fluor 594-labeled goat anti-rabbit IgG (H + L) (Catalog No. 2165334, Invitrogen, USA), goat anti-mouse IgG-HRP (Catalog No. 31430, Invitrogen, USA), goat anti-rabbit IgG (H + L)-HRP (Catalog No. 32460, Invitrogen, USA), and a protease inhibitor cocktail (Catalog No. EO0492, Thermo Scientific, USA).

Techniques: Infection, Expressing, Western Blot, Control, Comparison, Virus

Journal: mBio

Article Title: Sp1 facilitates continued HSV-1 gene expression in the absence of key viral transactivators

doi: 10.1128/mbio.03479-23

Figure Lengend Snippet: Antibodies for immunoblot experiments

Article Snippet: ICP0 , East Coast Bio , H1A027.

Techniques: Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Cytosolic nucleic acid sensing triggers type I interferon activation via the Hippo kinase LATS1

doi: 10.1016/j.jbc.2026.111204

Figure Lengend Snippet: Virus infection and cytosolic nucleic acid sensing trigger Hippo pathway activation in a LATS1-dependent manner. A and B , quantitative real-time PCR (qPCR) analysis of TEAD regulated genes Ctgf and Cyr61 in murine embryonic fibroblast (MEF) cells after infection with ( A ) vesicular stomatitis virus (VSV) (MOI 0.75; 24 h) or ( B ) herpes simplex virus-1 (HSV-1) (MOI 0.5; 8 h). C and D , qPCR of Ctgf and Cyr61 expression in MEF cells transfected with pI:C ( C ) or ISD ( D ) (2 μg/ml each; 3 h). E and F , immunoblot analysis ( left panels ) and relative quantification ( right panels ) of YAP expression in WT or Lats1 −/− MEF cells transfected with pI:C ( E ) or ISD ( F ) (2 μg/ml each; 8 h). G and H , immunoblot analysis ( left panels ) and relative quantification ( right panels ) of LATS1 (T1079) and IRF3 (S396) phosphorylation in MEF cells transfected with pI:C ( G ) or ISD ( H ) (2 μg/ml each) for the indicated times. Statistical significance was determined using Student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

Article Snippet: Primary antibodies used in this study were FLAG (Sigma Life Sciences); VSV-G, HSV-1 ICP4, HSP90, P65 (Santa Cruz Biotechnology); TBK1, p-TBK1, IRF3, p-IRF3, LATS1, p-LATS1, p-YAP, YAP/TAZ, STING, p-STING, p-P65, β-Actin (Cell Signaling Technology); β-Tubulin, GAPDH, HA (Proteintech); P65 (Santa Cruz Biotechnology).

Techniques: Virus, Infection, Activation Assay, Real-time Polymerase Chain Reaction, Expressing, Transfection, Western Blot, Quantitative Proteomics, Phospho-proteomics

Journal: The Journal of Biological Chemistry

Article Title: Cytosolic nucleic acid sensing triggers type I interferon activation via the Hippo kinase LATS1

doi: 10.1016/j.jbc.2026.111204

Figure Lengend Snippet: LATS1 is required for IFN-I activation. A and B , qPCR analysis of Ifnb mRNA expression in WT and Lats1 −/− MEF cells infected with VSV (MOI 0.5) ( A ) or HSV-1 (MOI 0.1) for 24 h. C and D , qPCR analysis of Cxcl10 and Ccl5 mRNA in WT and Lats1 −/− MEF cells infected with VSV as in panel A ( C ) or HSV-1 as in panel B ( D ). E and F , immunoblot analysis ( left panels ) and relative quantification ( right panels ) of IRF3 (S396) phosphorylation in WT and Lats1 −/− MEF cells transfected with pI:C ( E ) or ISD ( F ) (2 μg/ml each) for the indicated times. G and H , qPCR analysis of Ifnb mRNA expression in WT and Lats1 −/− MEF cells transfected with pI:C ( G ) or ISD ( H ) (2 μg/ml each) for the indicated times. I and J , ELISA for IFN-β secretion in WT and Lats1 −/− MEF cells transfected with pI:C ( I ) or ISD ( J ) (2 μg/ml each; 6 h). K and L , qPCR analysis of Cxcl10, Ccl5, and Isg15 mRNA in WT and Lats1 −/− MEF cells transfected with pI:C ( K ) or ISD ( L ) (2 μg/ml each) for the indicated times. Statistical significance was determined using Student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

Article Snippet: Primary antibodies used in this study were FLAG (Sigma Life Sciences); VSV-G, HSV-1 ICP4, HSP90, P65 (Santa Cruz Biotechnology); TBK1, p-TBK1, IRF3, p-IRF3, LATS1, p-LATS1, p-YAP, YAP/TAZ, STING, p-STING, p-P65, β-Actin (Cell Signaling Technology); β-Tubulin, GAPDH, HA (Proteintech); P65 (Santa Cruz Biotechnology).

Techniques: Activation Assay, Expressing, Infection, Western Blot, Quantitative Proteomics, Phospho-proteomics, Transfection, Enzyme-linked Immunosorbent Assay

Journal: The Journal of Biological Chemistry

Article Title: Cytosolic nucleic acid sensing triggers type I interferon activation via the Hippo kinase LATS1

doi: 10.1016/j.jbc.2026.111204

Figure Lengend Snippet: Cellular antiviral host defenses are dependent on LATS1. A and B , plaque assay for viral titers from supernatants of WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) ( A ) or HSV-1 (MOI 0.4) for 16 h. C , immunoblot analysis ( left panel ) and relative quantification ( right panel ) for VSV encoded glycoprotein, G (VSV-G) in WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) for the indicated times. D , immunoblot analysis ( left panel ) and relative quantification ( right panel ) of HSV-1 encoded immediate early transcription factor, ICP4 (HSV-1 ICP4) expression in WT and Lats1 −/− MEF cells infected with HSV-1 (MOI 0.1) for the indicated times. E and F , fluorescent imaging of WT and Lats1 −/− MEF cells infected with VSV-GFP (MOI 0.1) ( E ) or HSV-1-GFP (MOI 0.1) ( F ) for 24 h. Left to right : bright field, GFP, merged channels. Scale bar = 170 μm. Statistical significance was determined using student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

Article Snippet: Primary antibodies used in this study were FLAG (Sigma Life Sciences); VSV-G, HSV-1 ICP4, HSP90, P65 (Santa Cruz Biotechnology); TBK1, p-TBK1, IRF3, p-IRF3, LATS1, p-LATS1, p-YAP, YAP/TAZ, STING, p-STING, p-P65, β-Actin (Cell Signaling Technology); β-Tubulin, GAPDH, HA (Proteintech); P65 (Santa Cruz Biotechnology).

Techniques: Plaque Assay, Infection, Western Blot, Quantitative Proteomics, Expressing, Imaging