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Image Search Results
Journal: bioRxiv
Article Title: The carnosinase dipeptidase CNDP1 is a novel metabolic vulnerability in brain metastasis
doi: 10.1101/2025.03.18.644053
Figure Lengend Snippet: (A) 10-230 and 12-273 BM transduced with indicated tetON-shRNAs and treated with doxycycline during 72h, labeled with AHA (APC) for 4 hours and analyzed by flow cytometry. Cycloheximide (CHX, 50 ug/ml) was used as a positive control of translation shutdown. Statistics rendered by one-way ANOVA with Dunnet’s multiple hypothesis testing correction. (B) Left. Representative immunoblots of phospho-S6K1 p70 (Threonine 389), S6K1 p70, phosphoSer65 4E-BP, total 4EBP and housekeeping (HK) protein Hsp90 in lysates of 10-230 BM transduced with indicated tetON-shRNA. Right. Representative immunoblots of phospho-S6K1 p70 (Threonine 389), S6K1 p70, phosphoSer65 4E-BP, 4EBP and housekeeping (HK) protein Hsp90 in lysates of 10-230 BM cells transfected with siRNA NTC or CNDP1 as indicated. Band correspondent to p70 S6K1 is indicated. Results for HRI, phosphoSer51 and total eiF2α expression from same samples shown in and , respectively. Ratio of protein phosphorylation shown normalized to total protein and HK protein levels. See Document S1 for details. (C) GENI (gene set enrichment identifier124) was applied to TCGA melanoma samples (n = 472) using the Reactome 2022 pathway database. Five anticorrelating gene sets with FDR < 0.05 were represented. (D) Genes differentially expressed in shCNDP1 (combined) vs shNTC polysome heavy chain (log fc Translation vs log fc Transcription; p<0.05, lfc > 1) at translation, transcription and both transcription and translation levels in 10-230 BM cells. See Table 11. (E) Integrated Gene Enrichment analysis of Heavy Chain RNA sequencing of 10-230 BM shCNDP1 (combined) vs shNTC represented by Normalized Enrichment Score using Reactome Gene Ontology Analysis (RNA-seq: adjust p value < 0.05). See also
Article Snippet: RRID: AB_477617), Atf 4 (D4B8) Rabbit mAb (Cell Signaling Technology, Cat#11815S, RRID: AB_2616025), Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb (Cell Signaling Technology, Cat#9234T, RRID:AB_2269803), p70 S6 Kinase Ab (Cell Signaling Technology, Cat#9202, RRID: AB_AB_331676) 4E-BP1 (53H11) Rabbit mAb (Cell Signaling Technology, Cat#9644T, RRID:AB_2799054), Phospho-4E-BP1 (Ser65) Ab (Cell Signaling Technology, Cat#9451T, RRID: AB_330947),
Techniques: Transduction, Labeling, Flow Cytometry, Positive Control, Western Blot, shRNA, Transfection, Expressing, Phospho-proteomics, RNA Sequencing