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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: FOXC2-AS1/FOXC2 axis mediates matrix stiffness-induced trans-differentiation of hepatic stellate cells into fibrosis-promoting myofibroblasts
doi: 10.7150/ijbs.81581
Figure Lengend Snippet: Matrix stiffness induced HSCs activation, upregulated the expression of FOXC2 and facilitated its nuclear accumulation. (A-B) Primary human HSCs were plated on polyacrylamide hydrogels and used to perform IF staining, qRT-PCR and WB with quantitative data shown. Stiffness promoted HSCs activation and increased FN1, Col1A1, CTGF and α-SMA expression. Magnification is ×10, and scale bars = 50 μm. n =three independent experiments, ** P < 0.01, *** P < 0.001 by Student's t-test versus 0.4kPa. (C) 25.6 kPa stiffness facilitated HSCs migration. The scale bars = 50 μm. n =three independent experiments, *** P < 0.001 by Student's t-test versus 0.4kPa. (D) Analysis of RNA-seq data (GSE101343) present that compared with 0.4kPa, 25.6kPa stiffness greatly induced the expression of a transcription factor, named FOXC2. (E-G) qRT-PCR, subcellular fractionation analysis and IF staining displayed that matrix stiffness effectively induced the significant expression of FOXC2 and promoted the nuclear accumulation of FOXC2. n =three independent experiments, Magnification is ×40, and scale bars = 20 μm.** P < 0.01, *** P < 0.001 by Student's t-test versus 0.4kPa. (H-I) qRT-PCR and WB present that in vivo stiffness upregulated the HSCs activation markers and FOXC2 expression as well. n =three independent experiments, *** P < 0.001 by Student's t-test versus O.O. (olive oil).
Article Snippet: The primary
Techniques: Activation Assay, Expressing, Staining, Quantitative RT-PCR, Migration, RNA Sequencing, Fractionation, In Vivo