hsc4 cell line Search Results


hsc-4 cell line  (Corning Life Sciences)
90
Corning Life Sciences hsc-4 cell line
Hsc 4 Cell Line, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsc4+cell+line/hsc+4+cell+line/pmc05101486-101-4-13
Average 90 stars, based on 1 article reviews
hsc-4 cell line - by Bioz Stars, 2026-09
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hsc4 cell line  (Broad Institute Inc)
90
Broad Institute Inc hsc4 cell line
a BRD4 ChIP-seq profile at the SOX2 locus in KYSE140 cells with and without e1 repression. b Averaged BRD4 ChIP-seq profile, across BRD4 sites that harbor high-confidence SOX2 binding (*SOX2 ChIP-seq peaks containing SOX motifs) or the other BRD4 sites, in KYSE140 cells with and without e1 repression. c BRD4 ChIP-seq profile at the e1–e8 locus in LK2, NCI-H520, and <t>HSC4</t> cells with and without e1 repression. d Top: PhastCons scores (0:1 range) representing the conservation level of DNA sequences in the e1 enhancer. Middle: distribution of JASPAR DNA motifs identified in the e1 enhancer. Bottom: CRISPR cutting sites that overlap with the identified DNA motifs. e RT-qPCR measuring expression changes of SOX2 in KYSE140 and LK2 cells after CRISPR-mediated disruption of each of the identified DNA motifs. The expression level was normalized to the sgAAVS1 control. n = 2 biologically independent experiments. *: combinatorial CRISPR cutting of SOX (2nd), AP1, RUNX, and STAT (2nd) motifs in KYSE140 cells, or SOX (2nd), AP1, SNAIL, and TCF motifs in LK2 cells. f ChIP-qPCR showing the relative enrichment of BRD4 at e1–e5 in KYSE140 and LK2 cells after combinatorial CRISPR cutting of the selected motifs. ChIP enrichment was normalized to DNA concentration of each sample (measured by Qubit) and then to sonicated genomic input. n = 2 biologically independent experiments. g Presence of the candidate functional motifs in the e1–e5 enhancers. Source data are provided as a Source Data file.
Hsc4 Cell Line, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsc4+cell+line/hsc4+cell+line/pmc08654995-222-8-18
Average 90 stars, based on 1 article reviews
hsc4 cell line - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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a BRD4 ChIP-seq profile at the SOX2 locus in KYSE140 cells with and without e1 repression. b Averaged BRD4 ChIP-seq profile, across BRD4 sites that harbor high-confidence SOX2 binding (*SOX2 ChIP-seq peaks containing SOX motifs) or the other BRD4 sites, in KYSE140 cells with and without e1 repression. c BRD4 ChIP-seq profile at the e1–e8 locus in LK2, NCI-H520, and HSC4 cells with and without e1 repression. d Top: PhastCons scores (0:1 range) representing the conservation level of DNA sequences in the e1 enhancer. Middle: distribution of JASPAR DNA motifs identified in the e1 enhancer. Bottom: CRISPR cutting sites that overlap with the identified DNA motifs. e RT-qPCR measuring expression changes of SOX2 in KYSE140 and LK2 cells after CRISPR-mediated disruption of each of the identified DNA motifs. The expression level was normalized to the sgAAVS1 control. n = 2 biologically independent experiments. *: combinatorial CRISPR cutting of SOX (2nd), AP1, RUNX, and STAT (2nd) motifs in KYSE140 cells, or SOX (2nd), AP1, SNAIL, and TCF motifs in LK2 cells. f ChIP-qPCR showing the relative enrichment of BRD4 at e1–e5 in KYSE140 and LK2 cells after combinatorial CRISPR cutting of the selected motifs. ChIP enrichment was normalized to DNA concentration of each sample (measured by Qubit) and then to sonicated genomic input. n = 2 biologically independent experiments. g Presence of the candidate functional motifs in the e1–e5 enhancers. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: A predominant enhancer co-amplified with the SOX2 oncogene is necessary and sufficient for its expression in squamous cancer

doi: 10.1038/s41467-021-27055-4

Figure Lengend Snippet: a BRD4 ChIP-seq profile at the SOX2 locus in KYSE140 cells with and without e1 repression. b Averaged BRD4 ChIP-seq profile, across BRD4 sites that harbor high-confidence SOX2 binding (*SOX2 ChIP-seq peaks containing SOX motifs) or the other BRD4 sites, in KYSE140 cells with and without e1 repression. c BRD4 ChIP-seq profile at the e1–e8 locus in LK2, NCI-H520, and HSC4 cells with and without e1 repression. d Top: PhastCons scores (0:1 range) representing the conservation level of DNA sequences in the e1 enhancer. Middle: distribution of JASPAR DNA motifs identified in the e1 enhancer. Bottom: CRISPR cutting sites that overlap with the identified DNA motifs. e RT-qPCR measuring expression changes of SOX2 in KYSE140 and LK2 cells after CRISPR-mediated disruption of each of the identified DNA motifs. The expression level was normalized to the sgAAVS1 control. n = 2 biologically independent experiments. *: combinatorial CRISPR cutting of SOX (2nd), AP1, RUNX, and STAT (2nd) motifs in KYSE140 cells, or SOX (2nd), AP1, SNAIL, and TCF motifs in LK2 cells. f ChIP-qPCR showing the relative enrichment of BRD4 at e1–e5 in KYSE140 and LK2 cells after combinatorial CRISPR cutting of the selected motifs. ChIP enrichment was normalized to DNA concentration of each sample (measured by Qubit) and then to sonicated genomic input. n = 2 biologically independent experiments. g Presence of the candidate functional motifs in the e1–e5 enhancers. Source data are provided as a Source Data file.

Article Snippet: Squamous cancer cell lines KYSE140, KYSE70, LK2, NCI-H520, HSC4, TE1, TE10, SKMES1, and RERFLCAI were obtained from the Broad Institute Cancer Cell Line Encyclopedia (CCLE) project , .

Techniques: ChIP-sequencing, Binding Assay, CRISPR, Quantitative RT-PCR, Expressing, Disruption, Control, ChIP-qPCR, Concentration Assay, Sonication, Functional Assay