hsa Search Results


mir 145a 5p mimics  (MedChemExpress)
94
MedChemExpress mir 145a 5p mimics
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Mir 145a 5p Mimics, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/hsa-miR-145-5p+mimic/pmc12948252-454-1-25
Average 94 stars, based on 1 article reviews
mir 145a 5p mimics - by Bioz Stars, 2026-09
94/100 stars
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tbst w hsa  (Nanoprobes Inc)
86
Nanoprobes Inc tbst w hsa
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Tbst W Hsa, supplied by Nanoprobes Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/anti+hsa+tbst+w/pm12763070-56-45-49
Average 86 stars, based on 1 article reviews
tbst w hsa - by Bioz Stars, 2026-09
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bovine serum albumin bsa  (Valiant Co Ltd)
97
Valiant Co Ltd bovine serum albumin bsa
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Bovine Serum Albumin Bsa, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/Albumin/pmc13098520-49-0-7
Average 97 stars, based on 1 article reviews
bovine serum albumin bsa - by Bioz Stars, 2026-09
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neuronal  (Miltenyi Biotec)
94
Miltenyi Biotec neuronal
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Neuronal, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/CD24+Antibody%2C+anti-mouse%2C+REAfinity/10__1523_slash_eneuro__0348___21__2022-153-35-44
Average 94 stars, based on 1 article reviews
neuronal - by Bioz Stars, 2026-09
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vector pll3 7 hsa mir 150  (Addgene inc)
93
Addgene inc vector pll3 7 hsa mir 150
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Vector Pll3 7 Hsa Mir 150, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/pLL3%2E7_hsa-miR-150+(Plasmid+%2325792)/pmc08865640-188-3-19
Average 93 stars, based on 1 article reviews
vector pll3 7 hsa mir 150 - by Bioz Stars, 2026-09
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stem cell research 77 2024 103427 sequence  (Addgene inc)
92
Addgene inc stem cell research 77 2024 103427 sequence
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Stem Cell Research 77 2024 103427 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/LSB-hsa-miR-3144-5p+(Plasmid+%23103427)/pm38696852-40-0-14
Average 92 stars, based on 1 article reviews
stem cell research 77 2024 103427 sequence - by Bioz Stars, 2026-09
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uas ivs gfp p10  (Addgene inc)
92
Addgene inc uas ivs gfp p10
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Uas Ivs Gfp P10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/LSB-hsa-miR-485-5p+(Plasmid+%23103554)/pm33600999-122-31-32
Average 92 stars, based on 1 article reviews
uas ivs gfp p10 - by Bioz Stars, 2026-09
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low sensor backbone lsb  (Addgene inc)
92
Addgene inc low sensor backbone lsb
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Low Sensor Backbone Lsb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/LSB-hsa-miR-132-3p+(Plasmid+%23103219)/pmc08584665-195-22-27
Average 92 stars, based on 1 article reviews
low sensor backbone lsb - by Bioz Stars, 2026-09
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anti oxa1l  (Proteintech)
93
Proteintech anti oxa1l
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Anti Oxa1l, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/OXA1L+Antibody/bio_rxiv__2025__07__29__667405-145-16-18
Average 93 stars, based on 1 article reviews
anti oxa1l - by Bioz Stars, 2026-09
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homo sapiens serum albumin cdna  (OriGene)
90
OriGene homo sapiens serum albumin cdna
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Homo Sapiens Serum Albumin Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/Albumin+(ALB)+(NM_000477)+Human+Untagged+Clone/us09161966-798-0-8
Average 90 stars, based on 1 article reviews
homo sapiens serum albumin cdna - by Bioz Stars, 2026-09
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phage to dcas9 p2a hsa  (Addgene inc)
93
Addgene inc phage to dcas9 p2a hsa
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Phage To Dcas9 P2a Hsa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/pHAGE-TO-dCas9-P2A-HSA+(Plasmid+%23121936)/pm38519987-554-17-18
Average 93 stars, based on 1 article reviews
phage to dcas9 p2a hsa - by Bioz Stars, 2026-09
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mouse  (Miltenyi Biotec)
94
Miltenyi Biotec mouse
CircPTPN4 functions as a molecular sponge for <t>miR‐145a‐5p.</t> A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.
Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hsa/CD24+Antibody%2C+anti-mouse/pmc08451448-17-1-3
Average 94 stars, based on 1 article reviews
mouse - by Bioz Stars, 2026-09
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Image Search Results


CircPTPN4 functions as a molecular sponge for miR‐145a‐5p. A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.

Journal: Advanced Science

Article Title: Circular RNA PTPN4 Contributes to Blood‐Brain Barrier Disruption during Early Epileptogenesis

doi: 10.1002/advs.202502250

Figure Lengend Snippet: CircPTPN4 functions as a molecular sponge for miR‐145a‐5p. A) Schematic representation of the binding site in the CircPTPN4 seed region for miR‐145a‐5p. B) FISH assays showing the location of CircPTPN4 and miR‐145a‐5p in primary BMECs. C) Quantitative RT‐PCR analyses of CircPTPN4, GAPDH, and CircPCNX RNAs pulled down by biotinylated WT miR‐145a‐5p (Bio‐miR‐145 WT) and biotinylated mutant miR‐145a‐5p (Bio‐miR‐145 Mut), respectively ( n = 5; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPTPN4 versus GAPDH; p < 0.0001, Bio‐miR‐145 WT‐pulled down CircPCNX versus CircPTPN4; p < 0.0001, Bio‐miR‐145 Mut‐pulled down CircPTPN4 versus Bio‐miR‐145 WT‐pulled down CircPTPN4). D) RT‐PCR analyses of U6, miR‐145a‐5p, and miR‐28a‐5p RNAs pulled down by CircPTPN4 and random probe, respectively ( n = 5; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus U6; p < 0.0001, CircPTPN4 probe‐pulled down miR‐28a‐5p versus miR‐145a‐5p; p < 0.0001, CircPTPN4 probe‐pulled down miR‐145a‐5p versus random probe‐pulled down miR‐145a‐5p). Data are presented as means ± S.E.M. Statistical analysis was performed using two‐way ANOVA followed by Tukey's test. Scale bar = 10 µm.

Article Snippet: The miR‐145a‐5p mimics (HY‐ R00282 ), miRNA mimics negative Control (HY‐ R04602 ), miR‐145a‐5p inhibitor (HY‐RI00282), and miRNA inhibitor negative Control (HY‐RI04602) were purchased from Med Chem Express (MCE).

Techniques: Binding Assay, Quantitative RT-PCR, Mutagenesis, Reverse Transcription Polymerase Chain Reaction

CircPTPN4 disrupts tight junction by upregulating ECE‐1 expression via miR‐145a‐5p sponging in BMECs. A) Quantitative RT‐PCR analyses of ECE‐1 mRNA levels in BMECs co‐cultured with Control neurons or Mg 2 ⁺‐free neurons, and transduced with either Control‐ShRNA or CircPTPN4‐ShRNA lentivirus ( n = 5; p < 0.0001, ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Ctrl N BMECs; p = 0.0004, ShRNA‐CircPTPN4‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs). B) Western blot analyses of ECE‐1 protein levels in the cortex of Control and SE‐24 h mice infected with Control‐ShRNA or CircPTPN4‐ShRNA lentivirus. C) Bar graph quantifying ECE‐1 protein levels as the intensity ratio of ECE‐1 to GAPDH ( n = 5; p < 0.0001, ShRNA‐Ctrl‐treated SE‐24 h mice versus ShRNA‐Ctrl‐treated Ctrl mice; p < 0.0001, ShRNA‐CircPTPN4‐treated SE‐24 h mice versus ShRNA‐Ctrl‐treated SE‐24 h mice). D) Quantitative RT‐PCR analyses of ECE‐1 mRNA expression in Mg 2+ free N BMECs transduced with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐treated with anti‐miR Control or miR‐145a‐5p inhibitors ( n = 5; p < 0.0001, anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs versus Control anti‐miRNA and ShRNA‐Ctrl BMECs; p = 0.0048, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus Control anti‐miRNA and ShRNA‐CircPTPN4 BMECs; p < 0.0001, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs). E) Quantitative RT‐PCR analyses of ECE‐1 mRNA expression in Mg 2+ free N BMECs infected with OE‐Ctrl or OE‐CircPTPN4 lentivirus, and co‐treated with miR Control or miR‐145a‐5p mimics ( n = 5; p < 0.0001, miR Control and OE‐Ctrl BMECs versus miR‐145a‐5p mimics and OE‐Ctrl BMECs; p < 0.0001, miR Control and OE‐Ctrl BMECs versus miR Control and OE‐CircPTPN4 BMECs; p < 0.0001, miR Control and OE‐CircPTPN4 BMECs versus miR‐145a‐5p mimics and OE‐CircPTPN4 BMECs). F) Representative immunofluorescence images of Occludin staining in Mg 2+ free N BMECs infected with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐infected with anti‐miR Control or miR‐145a‐5p inhibitors. G) Bar graph showing quantification of Occludin staining intensity in Mg 2+ free N BMECs infected with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐infected with anti‐miR Control or miR‐145a‐5p inhibitors ( n = 5; p = 0.0066, anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs versus Control anti‐miRNA and ShRNA‐Ctrl BMECs; p = 0.0021, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus Control anti‐miRNA and ShRNA‐CircPTPN4 BMECs; p = 0.0049, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs). H) Bar graph showing the permeability of Mg 2+ free N BMECs transfected with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐treated with anti‐miR Control or miR‐145a‐5p inhibitors ( n = 5; p = 0.0021, anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs versus Control anti‐miRNA and ShRNA‐Ctrl BMECs; p = 0.0045, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus Control anti‐miRNA and ShRNA‐CircPTPN4 BMECs; p = 0.0002, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs). I) Representative immunofluorescence images of Occludin staining in Mg 2+ free N BMECs co‐cultured with Control or Mg 2 ⁺‐free neurons, and infected with Control‐ShRNA or ECE‐1‐ShRNA lentivirus. J) Bar graph showing quantification of Occludin staining in Ctrl N BMECs or Mg 2 ⁺‐free N BMECs, and infected with Control‐ShRNA or ECE‐1‐ShRNA lentivirus ( n = 5; p < 0.0001, ShRNA‐ECE‐1‐treated Ctrl N BMECs versus ShRNA‐Ctrl‐treated Ctrl N BMECs; p =0 .0108, ShRNA‐ECE‐1‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs; p = 0.0004, ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Ctrl N BMECs; p < 0.0001, ShRNA‐ECE‐1‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐ECE‐1‐treated Ctrl N BMECs). Values represent means ± S.E.M. Statistical analysis were performed using one‐way ANOVA followed by Tukey's test. Scale bar = 25 µm.

Journal: Advanced Science

Article Title: Circular RNA PTPN4 Contributes to Blood‐Brain Barrier Disruption during Early Epileptogenesis

doi: 10.1002/advs.202502250

Figure Lengend Snippet: CircPTPN4 disrupts tight junction by upregulating ECE‐1 expression via miR‐145a‐5p sponging in BMECs. A) Quantitative RT‐PCR analyses of ECE‐1 mRNA levels in BMECs co‐cultured with Control neurons or Mg 2 ⁺‐free neurons, and transduced with either Control‐ShRNA or CircPTPN4‐ShRNA lentivirus ( n = 5; p < 0.0001, ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Ctrl N BMECs; p = 0.0004, ShRNA‐CircPTPN4‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs). B) Western blot analyses of ECE‐1 protein levels in the cortex of Control and SE‐24 h mice infected with Control‐ShRNA or CircPTPN4‐ShRNA lentivirus. C) Bar graph quantifying ECE‐1 protein levels as the intensity ratio of ECE‐1 to GAPDH ( n = 5; p < 0.0001, ShRNA‐Ctrl‐treated SE‐24 h mice versus ShRNA‐Ctrl‐treated Ctrl mice; p < 0.0001, ShRNA‐CircPTPN4‐treated SE‐24 h mice versus ShRNA‐Ctrl‐treated SE‐24 h mice). D) Quantitative RT‐PCR analyses of ECE‐1 mRNA expression in Mg 2+ free N BMECs transduced with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐treated with anti‐miR Control or miR‐145a‐5p inhibitors ( n = 5; p < 0.0001, anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs versus Control anti‐miRNA and ShRNA‐Ctrl BMECs; p = 0.0048, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus Control anti‐miRNA and ShRNA‐CircPTPN4 BMECs; p < 0.0001, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs). E) Quantitative RT‐PCR analyses of ECE‐1 mRNA expression in Mg 2+ free N BMECs infected with OE‐Ctrl or OE‐CircPTPN4 lentivirus, and co‐treated with miR Control or miR‐145a‐5p mimics ( n = 5; p < 0.0001, miR Control and OE‐Ctrl BMECs versus miR‐145a‐5p mimics and OE‐Ctrl BMECs; p < 0.0001, miR Control and OE‐Ctrl BMECs versus miR Control and OE‐CircPTPN4 BMECs; p < 0.0001, miR Control and OE‐CircPTPN4 BMECs versus miR‐145a‐5p mimics and OE‐CircPTPN4 BMECs). F) Representative immunofluorescence images of Occludin staining in Mg 2+ free N BMECs infected with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐infected with anti‐miR Control or miR‐145a‐5p inhibitors. G) Bar graph showing quantification of Occludin staining intensity in Mg 2+ free N BMECs infected with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐infected with anti‐miR Control or miR‐145a‐5p inhibitors ( n = 5; p = 0.0066, anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs versus Control anti‐miRNA and ShRNA‐Ctrl BMECs; p = 0.0021, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus Control anti‐miRNA and ShRNA‐CircPTPN4 BMECs; p = 0.0049, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs). H) Bar graph showing the permeability of Mg 2+ free N BMECs transfected with ShRNA‐Ctrl or ShRNA‐CircPTPN4 lentivirus, and co‐treated with anti‐miR Control or miR‐145a‐5p inhibitors ( n = 5; p = 0.0021, anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs versus Control anti‐miRNA and ShRNA‐Ctrl BMECs; p = 0.0045, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus Control anti‐miRNA and ShRNA‐CircPTPN4 BMECs; p = 0.0002, anti‐miR‐145a‐5p and ShRNA‐CircPTPN4 BMECs versus anti‐miR‐145a‐5p and ShRNA‐Ctrl BMECs). I) Representative immunofluorescence images of Occludin staining in Mg 2+ free N BMECs co‐cultured with Control or Mg 2 ⁺‐free neurons, and infected with Control‐ShRNA or ECE‐1‐ShRNA lentivirus. J) Bar graph showing quantification of Occludin staining in Ctrl N BMECs or Mg 2 ⁺‐free N BMECs, and infected with Control‐ShRNA or ECE‐1‐ShRNA lentivirus ( n = 5; p < 0.0001, ShRNA‐ECE‐1‐treated Ctrl N BMECs versus ShRNA‐Ctrl‐treated Ctrl N BMECs; p =0 .0108, ShRNA‐ECE‐1‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs; p = 0.0004, ShRNA‐Ctrl‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐Ctrl‐treated Ctrl N BMECs; p < 0.0001, ShRNA‐ECE‐1‐treated Mg 2 ⁺‐free N BMECs versus ShRNA‐ECE‐1‐treated Ctrl N BMECs). Values represent means ± S.E.M. Statistical analysis were performed using one‐way ANOVA followed by Tukey's test. Scale bar = 25 µm.

Article Snippet: The miR‐145a‐5p mimics (HY‐ R00282 ), miRNA mimics negative Control (HY‐ R04602 ), miR‐145a‐5p inhibitor (HY‐RI00282), and miRNA inhibitor negative Control (HY‐RI04602) were purchased from Med Chem Express (MCE).

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, Control, Transduction, shRNA, Western Blot, Infection, Immunofluorescence, Staining, Permeability, Transfection