Journal: Oncotarget

Article Title: Riluzole exerts distinct antitumor effects from a metabotropic glutamate receptor 1-specific inhibitor on breast cancer cells

doi: 10.18632/oncotarget.17961

Figure Lengend Snippet: Molecular characteristics and drug response of breast cancer cell lines

Article Snippet: MCF-7, T-47D, BT-474, MDA-MB-231, Hs578T, and BT-549 breast cancer cell lines (Charles River Laboratories, Inc, New York, NY; ATCC, Manassas, VA) were maintained in Dulbecco's Modified Eagle's medium (DMEM; Sigma Aldrich, St. Louis, MO) plus 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Life Technologies, Grand Island, NY) at 37°C in a humidified atmosphere containing 5% CO 2 .

Techniques: Expressing

Journal: Oncotarget

Article Title: Riluzole exerts distinct antitumor effects from a metabotropic glutamate receptor 1-specific inhibitor on breast cancer cells

doi: 10.18632/oncotarget.17961

Figure Lengend Snippet: ER+ (A) and ER- (B) breast cancer cells were treated with 50 μM riluzole or 50 μM BAY 36-7620 for 48 h. Cells were stained with an antibody specific to phospho-histone H3 at Ser10 (phospho-H3) as a marker for mitosis and detected by flow cytometry. DMSO was used as a vehicle control. * P < 0.05 compared to DMSO control. ** P < 0.005 compared to DMSO control. Data are represented as mean +/− SD. (C) Phospho-cdc2 (Tyr 15; p-cdc2) and cyclin B1 expression were measured by Western blot after 24 h (Hs578T, BT-549) or 48 h (MCF-7, T-47D, BT-474, MDA-MB-231) drug treatment. Representative blot is shown (Western blot densitometry shown in ). β-actin and cdc-2 served as loading controls for cyclin B1 and p-cdc2 respectively.

Article Snippet: MCF-7, T-47D, BT-474, MDA-MB-231, Hs578T, and BT-549 breast cancer cell lines (Charles River Laboratories, Inc, New York, NY; ATCC, Manassas, VA) were maintained in Dulbecco's Modified Eagle's medium (DMEM; Sigma Aldrich, St. Louis, MO) plus 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Life Technologies, Grand Island, NY) at 37°C in a humidified atmosphere containing 5% CO 2 .

Techniques: Staining, Marker, Flow Cytometry, Expressing, Western Blot

Journal: iScience

Article Title: Conserved role of FOXC1 in TNBC is parallel to FOXA1 in ER+ breast cancer

doi: 10.1016/j.isci.2024.110500

Figure Lengend Snippet: Identification of FOXC1 cofactors (A) Protein co-interactors of FOXC1 identified in Hs578t and BT-549 via RIME in two biological replicates, after subtracting those identified in negative IgG control. (B) Western blot depicting co-immunoprecipitation of FOXC1 using antibody against NR2F2 with input and IgG negative controls.

Article Snippet: Hs578t , AddexBio , C0006016, RRID:CVCL_0332.

Techniques: Control, Western Blot, Immunoprecipitation

Journal: iScience

Article Title: Conserved role of FOXC1 in TNBC is parallel to FOXA1 in ER+ breast cancer

doi: 10.1016/j.isci.2024.110500

Figure Lengend Snippet:

Article Snippet: Hs578t , AddexBio , C0006016, RRID:CVCL_0332.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Sequencing, Generated, Gene Expression, Mass Spectrometry, Knock-Out, Software, Blocking Assay