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Image Search Results
Journal: Journal of Immunology Research
Article Title: Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice
doi: 10.1155/2017/7407136
Figure Lengend Snippet: Expression, secretion, and residual enzymatic activity of inactivated forms of HIV reverse transcriptase RT1.14opt-in and RT1.14oil in transiently transfected HeLa cells. RT content was determined by Western blotting using protein calibration curves with signal quantification by ImageJ (dubbed WB, in μ g/10 5 cells) and by quantification of enzymatic activity against standard RT preparation using Lenti RT activity test (Cavidi, Sweden) (dubbed EA, in pg/10 5 cells). RT content in cell lysates is dubbed “in cells” and in cell culture fluids “secreted.” Specific activity was measured as a ratio of protein content evaluated using the enzymatic activity assay to RT content determined by Western blotting.
Article Snippet: RT activity in the cell lysates and cell culture fluids was assessed by
Techniques: Expressing, Activity Assay, Reverse Transcription, Transfection, Western Blot, Cell Culture, Enzyme Activity Assay, Variant Assay
Journal:
Article Title: Upregulation of Surface Feline CXCR4 Expression following Ectopic Expression of CCR5: Implications for Studies of the Cell Tropism of Feline Immunodeficiency Virus
doi: 10.1128/JVI.76.18.9242-9252.2002
Figure Lengend Snippet: Susceptibility of AH927 cells transduced with CXCR4 or CCR5 to cell-free virus infection with cell culture-adapted (PETF14) and primary (GL8414) strains of FIV and to syncytium formation following transfection with expression vectors carrying the PETF14 or GL8414 env gene. Productive infection was measured by the nonisotopic reverse transcriptase assay (values represent absorbance measured at 405 nm; shading denotes a value above threshold). Syncytium formation was scored as positive or negative. Feline T-cell line Mya-1 was included as a control for infection with GL8414.
Article Snippet: Supernatants were collected every 3 days and assayed for the production of p24 (PetCheck FIV antigen ELISA; Idexx Corp.) or reverse transcriptase with the
Techniques: Transduction, Infection, Cell Culture, Transfection, Expressing, Reverse Transcriptase Assay