Journal: Molecular and cellular therapies

Article Title: Neural stem cells: ready for therapeutic applications?

doi: 10.1186/2052-8426-2-31

Figure Lengend Snippet: Clinical trials involving stem cells for retinal dystrophies

Article Snippet: NCT01625559 , Safety and Tolerability of MA09-hRPE Cells in Patients With Stargardt’s Macular Dystrophy (SMD) , Recruiting , Stargardt’s Macular Dystrophy , Biological: MA09-hRPE implantation , CHA Bio & Diostech , Phase 1 , 3 , September 2012 , October 2014.

Techniques: Clinical Proteomics, Transplantation Assay, Derivative Assay

Journal: Journal of Translational Medicine

Article Title: DEL-1: a promising treatment for AMD-associated ER stress in retinal pigment epithelial cells

doi: 10.1186/s12967-024-04858-9

Figure Lengend Snippet: DEL-1 ameliorates ER stress in hRPE cells. A Western blot analysis depicting phosphorylated eIF2α and CHOP in hRPE cells treated with tunicamycin (1 μg/mL) and/or DEL-1 (0–1 μg/mL) for 24 h. B Western blot analysis depicting phosphorylated eIF2α and CHOP in hRPE cells treated with DEL-1 (0–2 μg/mL) for 24 h. Means ± SDs were calculated from three independent experiments in in vitro models. * P < 0.05 compared with the control. ! P < 0.05 compared with tunicamycin treatment

Article Snippet: Cultivation of primary hRPE cells obtained from Sciencell Research Laboratories (San Diego, CA, USA) was performed using optimized Epithelial Cell Medium (Sciencell Research Laboratories).

Techniques: Western Blot, In Vitro, Control

Journal: Journal of Translational Medicine

Article Title: DEL-1: a promising treatment for AMD-associated ER stress in retinal pigment epithelial cells

doi: 10.1186/s12967-024-04858-9

Figure Lengend Snippet: DEL-1 mitigates ER stress-induced VEGF expression and apoptosis in hRPE cells. A Cell viability assay in hRPE cells treated with tunicamycin (0–2 μg/mL) or DEL-1 (0–2 μg/mL) for 24 h. B Western blot analysis of VEGF in hRPE cells treated with tunicamycin (1 μg/mL) and/or DEL-1 (0–1 μg/mL) for 24 h. C Cell viability and caspase 3 activity assay in hRPE cells treated with tunicamycin (2 μg/mL) and/or DEL-1 (0–5 μg/mL) for 24 h. Means ± SDs were calculated from three independent experiments in in vitro models. * P < 0.05 compared with the control. ! P < 0.05 compared with tunicamycin (1 or 2 μg/mL) treatment

Article Snippet: Cultivation of primary hRPE cells obtained from Sciencell Research Laboratories (San Diego, CA, USA) was performed using optimized Epithelial Cell Medium (Sciencell Research Laboratories).

Techniques: Expressing, Viability Assay, Western Blot, Caspase-3 Activity Assay, In Vitro, Control

Journal: Journal of Translational Medicine

Article Title: DEL-1: a promising treatment for AMD-associated ER stress in retinal pigment epithelial cells

doi: 10.1186/s12967-024-04858-9

Figure Lengend Snippet: Involvement of AMPK in the effects of DEL-1 on ER stress, VEGF expression, and apoptosis in hRPE cells. A Western blot analysis of phosphorylated AMPK, CAMKK2 and LKB1 in hRPE cells treated with DEL-1 (0–1 μg/mL) for 24 h. B Western blot analysis of phosphorylated eIF2α, CHOP, and VEGF in AMPK siRNA-transfected hRPE cells treated with tunicamycin (1 μg/mL) and/or DEL-1 (1 μg/mL) for 24 h. C Cell viability and caspase 3 activity assay in AMPK siRNA-transfected hRPE cells treated with tunicamycin (2 μg/mL) and/or DEL-1 (1 μg/mL) for 24 h. Means ± SDs were calculated from three independent experiments in in vitro models. * P < 0.05 compared with the control. ! P < 0.05 compared with tunicamycin (1 or 2 μg/mL) treatment. # P < 0.05 compared with tunicamycin (1 or 2 μg/mL) and DEL-1 treatment

Article Snippet: Cultivation of primary hRPE cells obtained from Sciencell Research Laboratories (San Diego, CA, USA) was performed using optimized Epithelial Cell Medium (Sciencell Research Laboratories).

Techniques: Expressing, Western Blot, Transfection, Caspase-3 Activity Assay, In Vitro, Control

Journal: Journal of Translational Medicine

Article Title: DEL-1: a promising treatment for AMD-associated ER stress in retinal pigment epithelial cells

doi: 10.1186/s12967-024-04858-9

Figure Lengend Snippet: Autophagy-mediated signaling contributes to the protective effects of DEL-1 against ER stress-induced VEGF expression and injury in hRPE cells. A MDC staining and Western blot analysis of LC3 I/II and p62 in hRPE cells treated with DEL-1 (0–1 μg/mL) for 24 h. B Western blot analysis of phosphorylated eIF2α, CHOP, and VEGF in hRPE cells treated with tunicamycin (1 μg/mL), DEL-1 (1 μg/mL), and/or 3-MA (2 mM) for 24 h. C Cell viability and caspase 3 activity assay in hRPE cells treated with tunicamycin (1 μg/mL), DEL-1 (1 μg/mL), and/or 3-MA (2 mM) for 24 h. Means ± SDs were calculated from three independent experiments in in vitro models. * P < 0.05 compared with the control. ! P < 0.05 compared with tunicamycin (1 or 2 μg/mL) treatment. # P < 0.05 compared with tunicamycin (1 or 2 μg/mL) and DEL-1 treatment

Article Snippet: Cultivation of primary hRPE cells obtained from Sciencell Research Laboratories (San Diego, CA, USA) was performed using optimized Epithelial Cell Medium (Sciencell Research Laboratories).

Techniques: Expressing, Staining, Western Blot, Caspase-3 Activity Assay, In Vitro, Control

Journal: Journal of Translational Medicine

Article Title: DEL-1: a promising treatment for AMD-associated ER stress in retinal pigment epithelial cells

doi: 10.1186/s12967-024-04858-9

Figure Lengend Snippet: DEL-1 attenuates ER stress and apoptosis in primary hRPE cells. A Western blot analysis depicting phosphorylated eIF2α and CHOP in primary hRPE cells treated with tunicamycin (1 μg/mL) and/or DEL-1 (0–1 μg/mL) for 24 h. B Cell viability and caspase 3 activity assay in primary hRPE cells treated with tunicamycin (2 μg/mL) and/or DEL-1 (0–1 μg/mL) for 24 h. Means ± SDs were calculated from three independent experiments in in vitro models. * P < 0.05 compared with the control. ! P < 0.05 compared with tunicamycin (1 or 2 μg/mL) treatment

Article Snippet: Cultivation of primary hRPE cells obtained from Sciencell Research Laboratories (San Diego, CA, USA) was performed using optimized Epithelial Cell Medium (Sciencell Research Laboratories).

Techniques: Western Blot, Caspase-3 Activity Assay, In Vitro, Control

Journal: Journal of Translational Medicine

Article Title: DEL-1: a promising treatment for AMD-associated ER stress in retinal pigment epithelial cells

doi: 10.1186/s12967-024-04858-9

Figure Lengend Snippet: Illustration depicting the effects of the myokine DEL-1 on VEGF expression and apoptosis in hRPE cells

Article Snippet: Cultivation of primary hRPE cells obtained from Sciencell Research Laboratories (San Diego, CA, USA) was performed using optimized Epithelial Cell Medium (Sciencell Research Laboratories).

Techniques: Expressing