Journal: Cell chemical biology

Article Title: Synergy and Target Promiscuity Drive Structural Divergence in Bacterial Alkylquinolone Biosynthesis

doi: 10.1016/j.chembiol.2017.08.024

Figure Lengend Snippet: Key Resources Table

Article Snippet: HQNO , Santa Cruz Biotech , Cat#202654;CAS:34 1-88-8.

Techniques: Virus, Recombinant, Software

Journal: bioRxiv

Article Title: An effective response to respiratory inhibition by a Pseudomonas aeruginosa excreted quinoline promotes Staphylococcus aureus fitness and survival in co-culture

doi: 10.1101/2025.03.12.642861

Figure Lengend Snippet: Panels A and B. The wild type (WT; JMB1100) strain containing the pOS_ nrd_gfp (panel A) or pOS_ srrA_gfp (panel B) transcriptional reporters were co-cultured with different concentrations of HQNO before gfp expression was quantified using fluorimetry. Panels C and D. The WT and Δ srrAB (JMB1467) strains containing the pOS_ nrdD_gfp (panel C) or pOS_ srrA_gfp (Panel D) transcriptional reporter trains were cultured in TSB-Cm with and without 5 μg mL -1 HQNO before gfp expression was quantified using fluorimetry. Each individual data bar corresponds to the average of the biological replicates, and standard deviations are shown, but the data points sometimes obscure them. Student’s t-tests were performed and * denoting a p-value ≤ 0.05. BDL denotes below the detectable limit of the fluorimeter.

Article Snippet: Cells were treated with 5 μg mL -1 HQNO (Selleck Chemicals) prepared as a 5 mg mL -1 stock in DMSO or vehicle control.

Techniques: Cell Culture, Expressing

Journal: bioRxiv

Article Title: An effective response to respiratory inhibition by a Pseudomonas aeruginosa excreted quinoline promotes Staphylococcus aureus fitness and survival in co-culture

doi: 10.1101/2025.03.12.642861

Figure Lengend Snippet: Panel A. Oxygen consumption rates were quantified in the wild type (WT; JMB1100) and Δ cydA qoxB::tet (JMB 8988) strains after culture in TSB media with the presence or absence of 5 μg mL -1 HQNO. Panel B. Membrane potential was qualitatively assessed using 1 mM of the fluorescent dye DIOC 2 in the WT and Δ cydA qoxB : :tet strains after culture in TSB media with the presence or absence of 5 μg mL -1 HQNO. Panels C and D. The WT, Δ srrAB (JMB1467), Δ cydA qoxB : :tet , and Δ srrAB Δ cydA qoxB : :tet (JMB 8989) strains containing pOS_ nrdD_gfp or pOS_ srrA_gfp after culture in TSB media in the presence and absence of 5 μg mL -1 HQNO before gfp expression was quantified using fluorimetry. Each individual data bar corresponds to the average of the biological replicates, and standard deviations are shown, but the data points sometimes obscure them. Student’s t-tests were performed, and * denoting a p-value ≤ 0.05, and ns denotes no significant difference. BDL denotes below the detectable limit.

Article Snippet: Cells were treated with 5 μg mL -1 HQNO (Selleck Chemicals) prepared as a 5 mg mL -1 stock in DMSO or vehicle control.

Techniques: Membrane, Expressing

Journal: bioRxiv

Article Title: An effective response to respiratory inhibition by a Pseudomonas aeruginosa excreted quinoline promotes Staphylococcus aureus fitness and survival in co-culture

doi: 10.1101/2025.03.12.642861

Figure Lengend Snippet: Panel A. The relative abundances of NAD + and NADH in the wild type (WT; JMB1100) strain after co-culture in the presence and absence of 5 μg mL -1 HQNO. Panel B. The expression of gfp was monitored in the WT, Δ srrAB (JMB1467), Δ rex::kan (JMB 13604), and Δ srrAB Δ rex::kan (JMB 13626) strains containing pOS_ srrA_gfp after culture in the presence and absence of 5 μg mL -1 HQNO. The response to HQNO requires both SrrAB and Rex regulators. Each individual data bar corresponds to the average of the biological triplicates, and standard deviations are shown, but the data points sometimes obscure them. Student’s t-tests were performed, and * denoting a p-value ≤ 0.05, and ns denotes no significant difference. BDL denotes below the detectable limit.

Article Snippet: Cells were treated with 5 μg mL -1 HQNO (Selleck Chemicals) prepared as a 5 mg mL -1 stock in DMSO or vehicle control.

Techniques: Co-Culture Assay, Expressing

Journal: Applied and Environmental Microbiology

Article Title: Burkholderia thailandensis Methylated Hydroxyalkylquinolines: Biosynthesis and Antimicrobial Activity in Cocultures

doi: 10.1128/AEM.01452-20

Figure Lengend Snippet: Biosynthesis of 4-hydroxy-2-alkylquinoline congeners. Burkholderia thailandensis uses the hmq gene products to synthesize HAQs including HMAQ and HMAQ-NO. In Pseudomonas aeruginosa, the analogous pqs gene products synthesize the related compounds HAQ, HAQ-NO, and PQS. Shown are the N-oxidated species referred to in the text, HQNO and HMAQ-NO-C9 with a double bond at the 1′-2′ position added by HmqF. The B. thailandensis compounds are methylated by HmqG, which does not have a homolog in P. aeruginosa. PqsH is needed for production of PQS, which is specific to P. aeruginosa.

Article Snippet: HAQ-NO with a C 7 carbon chain (HQNO) and HAQ with a C 7 carbon chain (HHQ) were purchased from commercial sources (Cayman Chemicals and Sigma Aldrich, respectively). b No activity of any of the compounds against Pseudomonas aeruginosa strain PA14 or Escherichia coli strain JM109 was observed up to 200 μg/ml.

Techniques: Methylation