Journal: European Archives of Oto-Rhino-Laryngology

Article Title: Detection of antibody subclasses IgA, IgM and IgG against HPV L1 in HPV-positive oropharyngeal squamous cell carcinoma patients: a pilot study

doi: 10.1007/s00405-024-08537-9

Figure Lengend Snippet: Clinical characteristics of study population

Article Snippet: In addition to p16 IHC, a PCR-based subtype-specific HPV DNA detection (HPV 3.5 LCD Array KIT, Chipron ® , Germany) was performed from the histological tumor specimens at initial diagnosis.

Techniques:

Journal: European Archives of Oto-Rhino-Laryngology

Article Title: Detection of antibody subclasses IgA, IgM and IgG against HPV L1 in HPV-positive oropharyngeal squamous cell carcinoma patients: a pilot study

doi: 10.1007/s00405-024-08537-9

Figure Lengend Snippet: Excerpts of individual L1-antibody patterns. a Case 15: male, 57 years old, tonsil carcinoma, T2N1M0, HPV16 DNA pos., p16 pos., Therapy: RCT. b Case 17: male, 67 years old, tonsil carcinoma, T2N0M0, HPV16 DNA pos., p16 pos., Therapy: surgery + RT. c Case 18: male, 77 years old, tonsil carcinoma, HPV16 DNA pos., p16 pos., Therapy: RIT. d Case 27: female, 61 years old, tonsil carcinoma, HPV16 DNA pos., p16 pos., Therapy: surgery + RCT

Article Snippet: In addition to p16 IHC, a PCR-based subtype-specific HPV DNA detection (HPV 3.5 LCD Array KIT, Chipron ® , Germany) was performed from the histological tumor specimens at initial diagnosis.

Techniques:

Journal: Head and Neck Pathology

Article Title: Identification of Rare and Common HPV Genotypes in Sinonasal Papillomas

doi: 10.1007/s12105-020-01148-w

Figure Lengend Snippet: Exemplary agarose gel and LCD image. a An exemplary agarose gel of seven different tissue samples (A–G) from which HPV DNA was amplified using either the 125 primer pair or the MY09/MY 11 primer pair. MY09/MY11 PCR yields an amplicon of roughly 450 bp length while the 125 primer pair can also detect slightly degraded DNA fragments (amplicon length 125–155 bp). Both PCRs were performed with an additional positive control (sample with known HPV infection, " +". b An exemplary picture of an LCD plate with multiple wells for detection of different HPV genotypes. The color change in these four particular wells, after reverse hybridization, shows the tissue sample was infected by HPV genotypes 6 (marked in red) and 18 (blue)

Article Snippet: Two techniques were tested to distinguish between different HPV types: pyrosequencing using the HPV sign® PQ kit versus DNA microarray employing the HPV Type 3.5 LCD-array kit (Fig. b).

Techniques: Agarose Gel Electrophoresis, Amplification, Positive Control, Infection, Hybridization