Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: TGFbeta1-secretion and impact of tumor cell supernatants on the expression of alphaSMA, N-Cadherin and cytokeratin 19 (CK 19) in human perineural epithelial cells (HPNEC). A. Assessment of TGFbeta1-secretion in the supernatants of Panc1, SU86.86 and T3M4 via ELISA B. Representative western blot images of the alphaSMA content in perineural cells after conditioned media (CM) treatment. Graph representing the relative alphaSMA content in HPNEC after CM treatment with the supernatants of the different cancer cell lines. C. Representative western blot images of the N-Cadherin content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative N-Cadherin content of HPNEC after CM treatment with the supernatants of the different cancer cell lines. D. Representative western blot images of the CK19 content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative CK19 content of HPNEC after CM treatment with the supernatants of the different cancer cell lines.

Article Snippet: Human Perineural Epithelial Cells (HPNEC; ATCC CRL-4023) were cultured in Fibroblast Growth Medium (Innoprot, P60108) at 37 °C with 5 % CO2 until 70–80 % confluency.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: Transcriptional effects of SU86.86-conditioned media on human perineural epithelial cells Graph shows the gene expression profile of SU86.86-conditioned human perineural epithelial cells, as assessed with the he Human Epithelial to Mesenchymal Transition (EMT) RT² Profiler PCR Array. Significantly increased expression levels of upregulated genes are highlighted in red.

Article Snippet: Human Perineural Epithelial Cells (HPNEC; ATCC CRL-4023) were cultured in Fibroblast Growth Medium (Innoprot, P60108) at 37 °C with 5 % CO2 until 70–80 % confluency.

Techniques: Gene Expression, Expressing

Journal: bioRxiv

Article Title: Defining the in vivo mechanism of air pollutant toxicity using murine stress response biomarkers

doi: 10.1101/2022.10.05.510981

Figure Lengend Snippet: A . Triplicate HOTThet reporter mice were instilled with PM 10 from Marylebone St. and LacZ staining was performed in lungs (upper panels). Cryosections derived from the same samples were subjected to F4/80 immunohistochemistry (lower panels). B . Effect of 10 μg/mL Marylebone Road PM10 (MR-PM10) on PAFR expression in human primary nasal epithelial cells. Data are from 5 separate experiments and summarised as median fluorescent intensity (MFI). Figure represents the median value and p values are calculated by Mann Whitney test. C . Effect of 10 μg/mL Marylebone Road PM10 (MR-PM 10 ) on pneumococcal adhesion to human primary nasal epithelial cells (HPNEpC) and PAFR blocker CV3988 on MR-PM 10 stimulated pneumococcal adhesion to HPNEpC. Data are from 6 separate experiments. Data are summarised as median IQR Kruskal– Wallis with post hoc multiple comparison testing. D . Effect of anti-oxidant N-acetylcysteine on MR-PM10 stimulated pneumococcal adhesion to HPNEpC. Data are from 7 separate experiments and are summarised as median IQR Kruskal–Wallis with post hoc multiple comparison testing. Black arrow indicates reporter activation.

Article Snippet: Human primary nasal epithelial cells (HPNEpC) were purchased from PromoCell® (Heidelberg, Germany), and maintained in airway epithelial cell growth medium, with supplement kit, Primocin (InvivoGen, San Diego, USA), and 10% FBS.

Techniques: Staining, Derivative Assay, Immunohistochemistry, Expressing, MANN-WHITNEY, Activation Assay

Journal: The Journal of Allergy and Clinical Immunology: Global

Article Title: Curbside particulate matter and susceptibility to SARS–CoV-2 infection

doi: 10.1016/j.jacig.2023.100141

Figure Lengend Snippet: Effect of PM 10 on ACE2 expression. PM 10 was collected from the curbside of Marylebone Road (curbside PM 10 ) and the exhaust of a diesel car (diesel PM 10 ) by using the same cyclone. Cells were cultured with PM 10 for 2 hours, and ACE2 expression was determined by flow cytometry. The results are expressed as mean fluorescence intensity (MFI) adjusted for isotypic antibody control. A, Dose-response relationship between curbside PM 10 and ACE2 expression by A549 cells. Two data points are omitted for visual convenience. B , Replication of A549 dose-response data using 10 μg/mL of curbside PM 10 . C , Effect of curbside PM 10 and diesel PM 10 (10 μg/mL) on ACE2 expression (MFI) in purchased (Promocell) HPNEpCs. D, Effect of curbside PM 10 (10 μg/mL) on ACE2 expression in primary nasal epithelial cells obtained from a local adult donor HPNEpCs. E, Effect of curbside PM 10 (10 μg/mL) on ACE2 expression (MFI) in Calu3 cells. F, Effect of the antioxidant N-acetyl cysteine on expression of curbside PM 10 -stimulated ACE2 expression (MFI) in purchased HPNEpCs. Columns represent medians from at least 4 separate experiments. Data were compared by either the Mann-Whitney U test or Kruskal-Wallis test and Dunn multiple comparisons test.

Article Snippet: Human primary nasal epithelial cells (HPNEpCs) were either obtained from a nonsmoking, nonvaping, healthy adult donor by using a dental brush (donor HPNEpCs) or purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Flow Cytometry, Fluorescence, Control, MANN-WHITNEY

Journal: The Journal of Allergy and Clinical Immunology: Global

Article Title: Curbside particulate matter and susceptibility to SARS–CoV-2 infection

doi: 10.1016/j.jacig.2023.100141

Figure Lengend Snippet: Effect of 5% CSE on ACE2 expression in A549 cells ( A ) and purchased (from Promocell) HPNEpCs ( B ). Results are expressed as mean fluorescence intensity (MFI) adjusted for isotypic antibody control. Columns represent medians from at least 4 separate experiments, and data are compared by the Mann-Whitney U test.

Article Snippet: Human primary nasal epithelial cells (HPNEpCs) were either obtained from a nonsmoking, nonvaping, healthy adult donor by using a dental brush (donor HPNEpCs) or purchased from PromoCell (Heidelberg, Germany).

Techniques: Expressing, Fluorescence, Control, MANN-WHITNEY

Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation.

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: Fig. 2. TGFbeta1-secretion and impact of tumor cell supernatants on the expression of alphaSMA, N-Cadherin and cytokeratin 19 (CK 19) in human perineural epithelial cells (HPNEC). A. Assessment of TGFbeta1-secretion in the supernatants of Panc1, SU86.86 and T3M4 via ELISA B. Representative western blot images of the alphaSMA con tent in perineural cells after conditioned media (CM) treatment. Graph repre senting the relative alphaSMA content in HPNEC after CM treatment with the supernatants of the different cancer cell lines. C. Representative western blot images of the N-Cadherin content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative N-Cadherin content of HPNEC after CM treatment with the supernatants of the different cancer cell lines. D. Representative western blot images of the CK19 content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative CK19 content of HPNEC after CM treatment with the supernatants of the different cancer cell lines.

Article Snippet: The levels of MMP-3 and MMP-9 in the supernatants of HPNEC were quantified using Proteintech-KE00160 Human Total MMP-3 ELISA Kit and Proteintech-KE00164 Human MMP-9 ELISA Kit, respectively, according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot