Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: TGFbeta1-secretion and impact of tumor cell supernatants on the expression of alphaSMA, N-Cadherin and cytokeratin 19 (CK 19) in human perineural epithelial cells (HPNEC). A. Assessment of TGFbeta1-secretion in the supernatants of Panc1, SU86.86 and T3M4 via ELISA B. Representative western blot images of the alphaSMA content in perineural cells after conditioned media (CM) treatment. Graph representing the relative alphaSMA content in HPNEC after CM treatment with the supernatants of the different cancer cell lines. C. Representative western blot images of the N-Cadherin content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative N-Cadherin content of HPNEC after CM treatment with the supernatants of the different cancer cell lines. D. Representative western blot images of the CK19 content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative CK19 content of HPNEC after CM treatment with the supernatants of the different cancer cell lines.

Article Snippet: Human Perineural Epithelial Cells (HPNEC; ATCC CRL-4023) were cultured in Fibroblast Growth Medium (Innoprot, P60108) at 37 °C with 5 % CO2 until 70–80 % confluency.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: Transcriptional effects of SU86.86-conditioned media on human perineural epithelial cells Graph shows the gene expression profile of SU86.86-conditioned human perineural epithelial cells, as assessed with the he Human Epithelial to Mesenchymal Transition (EMT) RT² Profiler PCR Array. Significantly increased expression levels of upregulated genes are highlighted in red.

Article Snippet: Human Perineural Epithelial Cells (HPNEC; ATCC CRL-4023) were cultured in Fibroblast Growth Medium (Innoprot, P60108) at 37 °C with 5 % CO2 until 70–80 % confluency.

Techniques: Gene Expression, Expressing

Journal: Neoplasia (New York, N.Y.)

Article Title: Pancreatic cancer cells infiltrate nerves through TGFbeta1-driven perineural epithelial-to-mesenchymal-like transdifferentiation.

doi: 10.1016/j.neo.2025.101126

Figure Lengend Snippet: Fig. 2. TGFbeta1-secretion and impact of tumor cell supernatants on the expression of alphaSMA, N-Cadherin and cytokeratin 19 (CK 19) in human perineural epithelial cells (HPNEC). A. Assessment of TGFbeta1-secretion in the supernatants of Panc1, SU86.86 and T3M4 via ELISA B. Representative western blot images of the alphaSMA con tent in perineural cells after conditioned media (CM) treatment. Graph repre senting the relative alphaSMA content in HPNEC after CM treatment with the supernatants of the different cancer cell lines. C. Representative western blot images of the N-Cadherin content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative N-Cadherin content of HPNEC after CM treatment with the supernatants of the different cancer cell lines. D. Representative western blot images of the CK19 content in HPNEC after conditioned media (CM) treatment. Densitometry graph representing the relative CK19 content of HPNEC after CM treatment with the supernatants of the different cancer cell lines.

Article Snippet: The levels of MMP-3 and MMP-9 in the supernatants of HPNEC were quantified using Proteintech-KE00160 Human Total MMP-3 ELISA Kit and Proteintech-KE00164 Human MMP-9 ELISA Kit, respectively, according to the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot