Journal: Cancer Science

Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy

doi: 10.1111/cas.16173

Figure Lengend Snippet: ZKSCAN3 expression in PaCa cells and its relationship to the prognosis of PaCa patients. (A) ZKSCAN3 mRNA expression in HPDE cell line (H6c7) and PaCa cell lines (BxPC‐3, Capan‐2, PANC‐1, SW1990, AsPC‐1, and MIA PaCa‐2) were assessed using RT‐qPCR. The mRNA expression levels in each sample were normalized to the expression of GAPDH . (B) ZKSCAN3 protein expression levels in the HPDE cell line (H6c7) and PaCa cell lines (BxPC‐3, Capan‐2, PANC‐1, SW1990, AsPC‐1, and MIA PaCa‐2) were evaluated using western blotting. The band densities of ZKSCAN3 in western blots from each cell line were calculated relative to the band densities of GAPDH. Comparisons between HPDE cells and PaCa cell lines were assessed using one‐way analysis of variance. (C) The box plots illustrate the mRNA expression levels of ZKSCAN3 in PaCa tissues and normal pancreatic tissues obtained from the database in the UCSC Xena website ( https://xena.ucsc.edu , accessed 20 February 2024). (D) Kaplan–Meier curves depict OS of 177 PaCa patients and RFS of 69 PaCa patients from the database in the Kaplan–Meier plot website ( https://kmplot.com/analysis/index.php?p=service&cancer=pancancer_rnaseq , accessed May 28, 2022). * p < 0.05; ** p < 0.01 compared with H6c7.

Article Snippet: The HPDE cell line, H6c7, was purchased from Kerafast, Inc. AsPC‐1, BxPC‐3 and Capan‐2 cell lines were maintained in RPMI‐1640 medium (Sigma Aldrich; Merck KGaA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Reports Medicine

Article Title: CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches

doi: 10.1016/j.xcrm.2024.101692

Figure Lengend Snippet:

Article Snippet: HPDE , AddexBio , T0018001.

Techniques: Recombinant, Blocking Assay, Cell Recovery, cDNA Synthesis, Reverse Transcription, Sample Prep, Multiplexing, Membrane, Cell Viability Assay, Software, High Content Screening, Cell Culture