Journal: Molecules

Article Title: Effects of Hydroxypropyl-Beta-Cyclodextrin on Cultured Brain Endothelial Cells

doi: 10.3390/molecules27227738

Figure Lengend Snippet: Fluorescence microscopic images of FITC-HPBCD-treated and untreated hCMEC/D3 cells. (Green: claudin-5 immunostaining on immunohistochemistry image and FITC-HPBCD on FITC-HPBCD uptake image; blue: cell nuclei) Bar: 20 µm.

Article Snippet: After 120 min incubation, samples from all compartments were collected and the concentration of FITC-HPBCD and the marker molecules were determined with spectrofluorometer (Fluorolog 3, Horiba Jobin Yvon, Edison, NJ, USA).

Techniques: Fluorescence, Immunostaining, Immunohistochemistry

Journal: Molecules

Article Title: Effects of Hydroxypropyl-Beta-Cyclodextrin on Cultured Brain Endothelial Cells

doi: 10.3390/molecules27227738

Figure Lengend Snippet: Permeability of FITC-HPBCD (50 µM) and marker molecules (SF, EBA) across the primary-cell-based BBB model. Values presented are means ± SEM. Statistical analysis: one-way analysis of variance followed by Dunnett’s post-test. *** p < 0.001, compared to SF treated group, n = 4. (P app -apparent permeability coefficient).

Article Snippet: After 120 min incubation, samples from all compartments were collected and the concentration of FITC-HPBCD and the marker molecules were determined with spectrofluorometer (Fluorolog 3, Horiba Jobin Yvon, Edison, NJ, USA).

Techniques: Permeability, Marker

Journal: Molecules

Article Title: Effects of Hydroxypropyl-Beta-Cyclodextrin on Cultured Brain Endothelial Cells

doi: 10.3390/molecules27227738

Figure Lengend Snippet: Permeability of FITC-HPBCD (50 µM) and marker molecules (SF, FD) on hCMEC/D3 cells. Values presented are means ± SEM. Statistical analysis: one-way analysis of variance followed by Tukey’s multiple comparisons post-test. **** p < 0.0001, compared to SF treated group, n for SF (A-–B) = 8; FD (A–B) = 12; F-HPBCD (A–B) = 8; F-HPBCD (B–A) = 4. (P app —apparent permeability coefficient).

Article Snippet: After 120 min incubation, samples from all compartments were collected and the concentration of FITC-HPBCD and the marker molecules were determined with spectrofluorometer (Fluorolog 3, Horiba Jobin Yvon, Edison, NJ, USA).

Techniques: Permeability, Marker

Journal: Molecules

Article Title: Effects of Hydroxypropyl-Beta-Cyclodextrin on Cultured Brain Endothelial Cells

doi: 10.3390/molecules27227738

Figure Lengend Snippet: Uptake of FITC-HPBCD by hCMEC/D3 cells. Fluorescence intensity values of the samples are normalized to the value of the untreated control and presented as means ± SEM. Statistical analysis: unpaired t test with Welch’s correction (two-tailed p value). **** p < 0.0001, n = 12 for ‘37 °C’ and n = 13 for ‘on ice’.

Article Snippet: After 120 min incubation, samples from all compartments were collected and the concentration of FITC-HPBCD and the marker molecules were determined with spectrofluorometer (Fluorolog 3, Horiba Jobin Yvon, Edison, NJ, USA).

Techniques: Fluorescence, Two Tailed Test

Journal: Molecules

Article Title: Effects of Hydroxypropyl-Beta-Cyclodextrin on Cultured Brain Endothelial Cells

doi: 10.3390/molecules27227738

Figure Lengend Snippet: Fluorescence microscopic images of FITC-HPBCD containing intracellular vesicles and lysosomes in hCMEC/D3 cells after 30 min and 2 h of incubation. Blue: cell nuclei, green: FITC-HPBCD, red: lysosomes stained with Lysotracker, yellow pixels: colocalization of FITC-HPBCD and lysosomes. Bar: 20 µm.

Article Snippet: After 120 min incubation, samples from all compartments were collected and the concentration of FITC-HPBCD and the marker molecules were determined with spectrofluorometer (Fluorolog 3, Horiba Jobin Yvon, Edison, NJ, USA).

Techniques: Fluorescence, Incubation, Staining

Journal: Molecules

Article Title: Effects of Hydroxypropyl-Beta-Cyclodextrin on Cultured Brain Endothelial Cells

doi: 10.3390/molecules27227738

Figure Lengend Snippet: Results of flow cytometry analysis of lysosomal fluorescence of hCMEC/D3 cells after 30 min ( A ) or 2 h ( B ) of HPBCD and FITC-HPBCD incubation at 50 µM concentration. Red lysosomal fluorescence does not increase significantly after HPBCD or FITC-HPBCD treatments compared to untreated cells (n.s; p > 0.05 for both molecules). FITC-HPBCD accumulation significantly increases green cellular fluorescence compared to control cells (*** p < 0.001). Red fluorescence: LysoTracker, green fluorescence: FITC-HPBCD. Values are presented as means ± S.D. n = 4.

Article Snippet: After 120 min incubation, samples from all compartments were collected and the concentration of FITC-HPBCD and the marker molecules were determined with spectrofluorometer (Fluorolog 3, Horiba Jobin Yvon, Edison, NJ, USA).

Techniques: Flow Cytometry, Fluorescence, Incubation, Concentration Assay

Journal: Pharmaceutics

Article Title: Topical Artocarpus communis Nanoparticles Improved the Water Solubility and Skin Permeation of Raw A. communis Extract, Improving Its Photoprotective Effect

doi: 10.3390/pharmaceutics13091372

Figure Lengend Snippet: The formulation and water solubility, yield, and encapsulation efficiency of AHP.

Article Snippet: On the contrary, the significant enhancement of ACM permeated in AHP contributed to the presence of HPBCD with PVP , indicating that ACM can be delivered via an HPBCD with PVPK30 nanocarrier through the stratum corneum, with the possibility of exerting local effects to prevent skin diseases.

Techniques: Formulation, Solubility, Encapsulation

Journal: Pharmaceutics

Article Title: Topical Artocarpus communis Nanoparticles Improved the Water Solubility and Skin Permeation of Raw A. communis Extract, Improving Its Photoprotective Effect

doi: 10.3390/pharmaceutics13091372

Figure Lengend Snippet: SEM images of ( A ) raw ACM, ( B ) HPBCD, ( C ) PVPK30, ( D ) physical mixture ACM/PVPK30/HPBCD (1:18:10), and ( E ) AHP 1:18:10 and ( F ) particle morphology of AHP 1:18:10 under TEM observation.

Article Snippet: On the contrary, the significant enhancement of ACM permeated in AHP contributed to the presence of HPBCD with PVP , indicating that ACM can be delivered via an HPBCD with PVPK30 nanocarrier through the stratum corneum, with the possibility of exerting local effects to prevent skin diseases.

Techniques:

Journal: Pharmaceutics

Article Title: Topical Artocarpus communis Nanoparticles Improved the Water Solubility and Skin Permeation of Raw A. communis Extract, Improving Its Photoprotective Effect

doi: 10.3390/pharmaceutics13091372

Figure Lengend Snippet: Powder X-ray diffraction patterns of ACM, HPBCD, PVPK30, physical mixture, and AHP.

Article Snippet: On the contrary, the significant enhancement of ACM permeated in AHP contributed to the presence of HPBCD with PVP , indicating that ACM can be delivered via an HPBCD with PVPK30 nanocarrier through the stratum corneum, with the possibility of exerting local effects to prevent skin diseases.

Techniques:

Journal: Pharmaceutics

Article Title: Topical Artocarpus communis Nanoparticles Improved the Water Solubility and Skin Permeation of Raw A. communis Extract, Improving Its Photoprotective Effect

doi: 10.3390/pharmaceutics13091372

Figure Lengend Snippet: Fourier transform infrared (FTIR) spectra of raw ACM, HPBCD, PVPK30, physical mixture, and AHP.

Article Snippet: On the contrary, the significant enhancement of ACM permeated in AHP contributed to the presence of HPBCD with PVP , indicating that ACM can be delivered via an HPBCD with PVPK30 nanocarrier through the stratum corneum, with the possibility of exerting local effects to prevent skin diseases.

Techniques: Fourier Transform Infrared Spectroscopy