Journal: Journal of Radiation Research

Article Title: Radiation-induced lung fibrosis in a tumor-bearing mouse model is associated with enhanced Type-2 immunity

doi: 10.1093/jrr/rrv077

Figure Lengend Snippet: Expression of transcription factors for Type-1/Type-2 immune response up to 21 days. T-bet ( A ) and GATA-3 ( B ) mRNA expression in RT group, LRT group and CRT group mice was measured by RT-PCR. ( C ) The difference between T-bet and GATA-3 protein expression was measured by western blot. Data are shown as mean ± SD. * P < 0.05.

Article Snippet: Polymerase chain reaction (PCR) primers for murine GATA-3 , T-bet and the housekeeping gene β-actin were designed by ShineGene Co. Ltd, based on cDNA sequences obtained from the GenBank database.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Molecular Biology Reports

Article Title: Human papillomavirus E6 alters Toll-like receptor 9 transcripts and chemotherapy responses in breast cancer cells in vitro

doi: 10.1007/s11033-024-10143-1

Figure Lengend Snippet: GFP and E6 expression in transduced breast cancer cells. The growth patterns and GFP signaling of MDA-MB-231 ( a ) and T-47D ( b ) cells after antibiotic selection and FACS. Imaging was conducted with IncuCyte S3 (10x). HPV16 E6 expression in E6-transduced cells was verified with qPCR ( c ), in which GAPDH was used for normalization. Results are presented as relative normalized expression (mean ± SEM, n = 9, * p < 0.05, ** p < 0.001)

Article Snippet: Custom E6 and housekeeping gene GAPDH primers were purchased from Eurogentec (Belgium) and TLR9 primers from Bio-Rad.

Techniques: Expressing, Selection, Imaging

Journal: Molecular Biology Reports

Article Title: Human papillomavirus E6 alters Toll-like receptor 9 transcripts and chemotherapy responses in breast cancer cells in vitro

doi: 10.1007/s11033-024-10143-1

Figure Lengend Snippet: The effect of E6 expression on TLR9 in breast cancer cells. The level of TLR9 mRNA ( a ) and protein ( b-c ) was studied after 96 h of incubation in normoxia and hypoxia in MDA-MB-231 and T-47D cells. GAPDH was used for normalization in both RT-qPCR and Western Blot. Blot images are cropped. Results are presented as mean ± SEM, n = 9, * p < 0.05

Article Snippet: Custom E6 and housekeeping gene GAPDH primers were purchased from Eurogentec (Belgium) and TLR9 primers from Bio-Rad.

Techniques: Expressing, Incubation, Quantitative RT-PCR, Western Blot