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Image Search Results
Journal: Veterinary Pathology
Article Title: Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma
doi: 10.1177/03009858211042588
Figure Lengend Snippet: Optimized concentrations of antibody for flow cytometric evaluation of cells in equine bronchoalveolar lavage fluid.
Article Snippet: The fluorescent primary antibodies used in this study were phycoerythrin (PE) conjugated mouse anti-human CD206 (clone 3.29B1, Beckman Coulter), fluorescein isothiocyanate (FITC) conjugated mouse anti-horse CD5 (clone CVS5, Bio-Rad), and
Techniques: Concentration Assay
Journal: Veterinary Pathology
Article Title: Flow cytometric analysis of equine bronchoalveolar lavage fluid cells in horses with and without severe equine asthma
doi: 10.1177/03009858211042588
Figure Lengend Snippet: Flow cytometric analysis of equine bronchoalveolar lavage fluid (BALF) leukocytes. (a) Indicates that cells from p1 have higher forward scatter (FSC) and side scatter (SSC) than cells from p2, indicating they are larger and more internally complex. (b) Unstained control sample showing that many (red circle) but not all cells from p1 (upper panel) have autofluorescence in the phycoerythrin (PE) and fluorescein isothiocyanate (FITC) channels. However, cells from p2 (lower panel) are not autofluorescent. (c) Using anti-CD90 (neutrophil marker) and anti-CD206 (macrophage marker) antibodies, cells from p1 (upper panel) but not p2 (lower panel) are identified as neutrophils (Q3) and macrophages (Q1), respectively. (d) Using antibodies against CD5 (lymphocyte marker) and PanB cells, cells in p1 (upper panel) are double-negative, and cells in p2 (lower panel) are identified as lymphocytes; the red circle indicates autofluorescent cells.
Article Snippet: The fluorescent primary antibodies used in this study were phycoerythrin (PE) conjugated mouse anti-human CD206 (clone 3.29B1, Beckman Coulter), fluorescein isothiocyanate (FITC) conjugated mouse anti-horse CD5 (clone CVS5, Bio-Rad), and
Techniques: Control, Marker