Journal: PLoS ONE

Article Title: Susceptibility of Human Head and Neck Cancer Cells to Combined Inhibition of Glutathione and Thioredoxin Metabolism

doi: 10.1371/journal.pone.0048175

Figure Lengend Snippet: A: Exponential growing and confluent FaDu, Cal-27 and SCC-25 cells were treated with 0.5 µM AUR and/or 1 mM BSO for 24 h and analyzed for clonogenic survival. Clonogenic cell survival data were normalized to exponentially growing and confluent control cells (not shown). B: FaDu and HNEpC cells were treated with BSO+AUR and the number of viable attached cells was counted after 24 h. Numbers of viable BSO+AUR-treated cells were normalized to their respective controls (CON). Error bars represent the standard error of the mean (SEM) of N = 3 experiments. *, p<0.05 versus EXP; ¥, p<0.05 versus CON.

Article Snippet: HNEpC cells were obtained from PromoCell (Heidelberg, Germany).

Techniques:

Journal: Nucleic acids research

Article Title: Cellular APOBEC3A deaminase drives mutations in the SARS-CoV-2 genome.

doi: 10.1093/nar/gkac1238

Figure Lengend Snippet: Figure 3. A3A mRNA expression in human airway and lung cells. (A) The A3A mRNA level in each cell line was quantified by RT–ddPCR 18 h after treatment without (control) or with IFN-ß and/or TNF- under normoxic conditions (N). A549, Calu-3, SAE, AT2 and LBE cells and HNEpCs were also cultured under hypoxic conditions (H). The mean mRNA copy num- bers of A3A relative to those of the housekeeping gene RPP40 are shown (n = 3). (B) The CD11b and CD68 mRNA levels in each untreated cell line were quantified by RT–ddPCR. Human MDMs were used as positive controls. The mean mRNA copy numbers of CD11b and CD68 relative to those of RPP40 are shown (n = 3). The error bars indicate the + SD values.

Article Snippet: Human nasal epithelial primary cells (HNEpCs) and human type II alveolar epithelial (AT2) cells were purchased from PromoCell and Accegen Biotechnology, respectively.

Techniques: Expressing, Control, Cell Culture

Journal: eBioMedicine

Article Title: Underground railway particulate matter and susceptibility to pneumococcal infection

doi: 10.1016/j.ebiom.2022.104063

Figure Lengend Snippet: Effect of 20 µg/mL London Underground particulate matter less than 10 microns from microns from Bakerloo (B-PM 10 ) and Jubilee lines (J-PM 10 ) on human primary epithelial cells. Pneumococcal adhesion to (a) human primary nasal epithelial cells (HPNEpC B-PM 10 and J-PM 10 n=5, technical replicates (TR)=3, p=0.007 and p=0.032, respectively, and (b) primary bronchial epithelial cells (HPBEpC; B-PM 10 and J-PM 10 n=5, TR=3, p=0.017 and p=0.014, respectively). Platelet-activating factor receptor (PAFR) median fluorescence intensity (MFI) expression, (c) HPNEpC (B-PM 10 and J-PM 10 , n=5, TR=2, p= 0.011 and p=0.02, respectively) and, (d) HPBEpC (B-PM 10 and J-PM 10 , n=5, TR=2, p=0.013 and p=0.003, respectively. Columns represents median and p values are calculated by Kruskal-Wallis with post hoc multiple comparison testing.

Article Snippet: Human primary nasal epithelial cells (HPNEpC), and human primary bronchial epithelial cells (HPBEpC) and were purchased from PromoCell® (Heidelberg, Germany), and maintained in airway epithelial cell growth medium, with supplement kit, Primocin (InvivoGen, San Diego, USA), and 10% FBS.

Techniques: Fluorescence, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Triterpenoid Saponins and Flavonoid Glycosides from the Flower of Camellia flavida and Their Cytotoxic and α-Glycosidase Inhibitory Activities

doi: 10.3390/ijms252010977

Figure Lengend Snippet: Cytotoxicity (IC 50 , μM ± SD, n = 8) of compounds 1 – 10 against cell lines.

Article Snippet: The human cervical cancer cell line Hela, the human lung adenocarcinoma cell line A549, the human breast cancer cell lines MCF-7 and MDA-MB-231, the human hepatoma cell lines BEL-7402 and Hep G2, the human embryonic lung fibroblast cell line MRC-5, and the human nasal epithelial cell line HNEpC were all sourced from the China Center for Type Culture Collection (CCTCC, Wuhan University, Hubei, China).

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