Journal: Journal of Hematology & Oncology

Article Title: Challenges and advances in clinical applications of mesenchymal stromal cells

doi: 10.1186/s13045-021-01037-x

Figure Lengend Snippet: The tests of modified MSCs using CRISPR-Cas9 technology

Article Snippet: Immortalized human bone marrow MSC cell line (ATCC PCS-500–041) , PUMILIO2 (PUM2) , Depletion of PUM2 blocks MSC adipogenesis and enhances osteogenesis. PUM2 works as a negative regulator on the 3′ UTRs of JAK2 and RUNX2 via direct binding. CRISPR/CAS9-mediated gene silencing of Pum2 inhibited lipid accumulation and excessive bone formation , [ ] .

Techniques: Modification, CRISPR, Functional Assay, Synthesized, In Vitro, Transfection, Expressing, Transplantation Assay, Inhibition, Binding Assay

Journal: Molecular Therapy. Nucleic Acids

Article Title: lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA

doi: 10.1016/j.omtn.2019.08.027

Figure Lengend Snippet: lncRNA ADAMTS9-AS2 Is Upregulated during hMSC Chondrogenic Differentiation (A) Isolated hMSCs from bone marrow and Alcian blue staining for hMSC micromass chondrogenesis on 14 days. (B) mRNA level of chondrogenic genes (Sox9, Col2α1, and ACAN) during chondrogenic differentiation on 14 days. (C) Heatmaps of lncRNA differentially expressed during hMSC chondrogenesis. (D) Expression of the upregulated and downregulated lncRNAs by qPCR. (E) qPCR for the expression of ADAMTS9-AS2 during chondrogenic differentiation at the indicated time points. (F) RT-PCR analysis indicating ADAMTS9-AS2 localization in the nucleus and the cytoplasm. Experiments were performed in triplicate and error bars represent SD of a triplicate set of experiments. Data are shown as mean ± SD; *p, 0.05; **p, 0.01; ns, non-significant.

Article Snippet: Sections of hMSC micromass (5 mm) or osteochondral constructs (6 mm) were subjected to immunohistochemistry.

Techniques: Isolation, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Molecular Therapy. Nucleic Acids

Article Title: lncRNA ADAMTS9-AS2 Controls Human Mesenchymal Stem Cell Chondrogenic Differentiation and Functions as a ceRNA

doi: 10.1016/j.omtn.2019.08.027

Figure Lengend Snippet: ADAMTS9-AS2 Promotes hMSC Chondrogenic Differentiation (A) Forced expression of ADAMTS9-AS2 in hMSCs led to a significant increase of the transcript, as measured by qPCR. (B–D) Expression of chondrogenic genes measured by qPCR at representative time points in hMSCs during chondrogenic differentiation between control and ADAMTS9-AS2 overexpression group. (E) Synthetic glycosaminoglycan in ADAMTS9-AS2-overexpressed hMSCs compared to controls on day 14, assessed by Safranine O staining, Alcian blue staining, and toluidine blue staining. Scale bar, 100 μm. (F) Antibody staining of paraffin-embedded sections of day 14 micromass showed Col2α1, ACAN, and ColX abundance between control and ADAMTS9-AS2 overexpression group. Scale bar, 50 μm. (G–I) Quantitative statistics for dyeing results of (F) between control and ADAMTS9-AS2 overexpression group. Experiments were performed in triplicate and error bars represent SD of a triplicate set of experiments. Data are shown as mean ± SD; *p, 0.05; **p, 0.01; ns, non-significant.

Article Snippet: Sections of hMSC micromass (5 mm) or osteochondral constructs (6 mm) were subjected to immunohistochemistry.

Techniques: Expressing, Control, Over Expression, Staining