Journal: The FASEB Journal

Article Title: Vimentin‐mediated myosin 10 aggregation at tips of cell extensions drives MT1‐MMP‐dependent collagen degradation in colorectal cancer

doi: 10.1096/fj.202300672r

Figure Lengend Snippet: FIGURE 1 Vimentin colocalizes with myosin 10 in progressive CRC. (A) Representative images of colon tissue stained with Vim (red), Myo10 (green), and their colocalization (yellow) areas in (A) normal (control) tissue, (B) low-grade, and (C) high-grade adenocarcinoma (tumor). Scale bar 500 μm for merge image and 100 μm for separate channels, objective 20x. (D and E) Quantification of Pearson colocalization for Vim and Myo10 in normal, low-grade (gray; LG), and high-grade (black; HG) tumor estimated for (D) epithelium and (E) stromal region. Mean fluorescence intensity (MFI) for (F) Vim and (G) Myo10 was measured separately in epithelial and stromal regions of normal and tumor tissue specimens (n = 26 for normal, n = 5 for LG, and n = 8 for HG tumor samples. Paired t-test was calculated for normal versus tumor samples for each patient separately).

Article Snippet: Plasmids containing pEGFP- Myo10 (#87256) and mTagRFP- T- Vimentin (#58029) were obtained from Addgene (Cambridge, MA).

Techniques: Staining, Control, Fluorescence

Journal: The FASEB Journal

Article Title: Vimentin‐mediated myosin 10 aggregation at tips of cell extensions drives MT1‐MMP‐dependent collagen degradation in colorectal cancer

doi: 10.1096/fj.202300672r

Figure Lengend Snippet: FIGURE 3 Vimentin regulates myosin10 substitution dynamics. (A) Representative live cell images for FRAP in SW480 cells transfected with GFP-Myo10. Images before bleaching (Pre-bleach), after bleach (Bleach), at intermediate recovery point (Half-recovery), and at the end of record fluorescent recovery (End-recovery); regions of interest (ROIs: 2 μm2 area indicated with insets). Fluorescence in ROIs was measured before bleaching and for 150 s after bleaching with argon laser at 488 nm. Cells were cultured on fibrillar Col-coated surface for 3 h. (B and C) Typical normalized FRAP curves are displayed in (B) SW480 (indigo) and SW480 KD (blue), and (C) mEF WT (gray), KO (red), and Vim rescue (magenta). Scale bar 10 μm. (D) FRAP mobile fractions, and (E) The FRAP-half-life recovery. All FRAP images were quantified with ImageJ (n = 3, at least 15 cells per group).

Article Snippet: Plasmids containing pEGFP- Myo10 (#87256) and mTagRFP- T- Vimentin (#58029) were obtained from Addgene (Cambridge, MA).

Techniques: Transfection, Fluorescence, Cell Culture

Journal: The FASEB Journal

Article Title: Vimentin‐mediated myosin 10 aggregation at tips of cell extensions drives MT1‐MMP‐dependent collagen degradation in colorectal cancer

doi: 10.1096/fj.202300672r

Figure Lengend Snippet: FIGURE 5 Vimentin colocalizes with MT1-MMP in progressive CRC. (A) Representative images of colon tissue staining with DAPI (blue), Vim (red), MT1-MMP (green), and their colocalization (magenta) areas in normal (control) tissue, low-grade, and high-grade adenocarcinoma (tumor). Images were obtained with a Zeiss Axio Scan.Z1 microscope, objective 20×. Scale bar: 100 μm. (B and C) Quantification of Pearson colocalization for Vim and MT1-MMP in normal tissue, low-grade (gray; LG), and high-grade (black; HG) tumor estimated for (B) epithelial and (C) stromal regions. (D) Representative images of MT1-MMP-Vim interaction studied by PLA (red dots) in SW480 and mEF WTs cells transfected with GFP-Myo10 plasmid (green). (E) Quantification of colocalization of PLA dots and Myo10 in SW480 and mEF cells. Paired t-test calculated for three biological replicates (presented by circle, square, and triangle symbols) with at least 15 cells per condition. (F) Representative images of Myo10-Vim interaction studied by PLA (white dots) in SW480 and mEF WTs cells, with and without Vim. (G) Quantification of positive PLA signals (Dots per cell) are shown that Vim depletion significantly reduced the number of positive PLA signals in SW480 (p < .0001) and mEF (p < .0001). Paired t-test was calculated for three biological replicates with at least 15 cells per condition. (H) MT1-MMP and Myo10 immunoprecipitations (IP) in mEF WT cells. IP of MT1-MMP was immunoblotted for Myo10. Input shows total abundance of proteins in cell lysate, and actin was used as a loading control.

Article Snippet: Plasmids containing pEGFP- Myo10 (#87256) and mTagRFP- T- Vimentin (#58029) were obtained from Addgene (Cambridge, MA).

Techniques: Staining, Control, Microscopy, Transfection, Plasmid Preparation

Journal: The FASEB Journal

Article Title: Vimentin‐mediated myosin 10 aggregation at tips of cell extensions drives MT1‐MMP‐dependent collagen degradation in colorectal cancer

doi: 10.1096/fj.202300672r

Figure Lengend Snippet: FIGURE 6 Model of Vim-Myo10-MT1-MMP complex-dependent collagen remodeling and ECM degradation. After initial cell extension formation, Vim binds to Myo10, which enhances Myo10 aggregation at the tips of cell extensions. This Vim-Myo10 complex is associated with MT1-MMP facilitating its movement toward the plasma membrane. MT1-MMP at the plasma membrane enhances collagen proteolysis and reorganization of the Col fibers, enhancing ECM degradation. Diagram was created with BioRender.com.

Article Snippet: Plasmids containing pEGFP- Myo10 (#87256) and mTagRFP- T- Vimentin (#58029) were obtained from Addgene (Cambridge, MA).

Techniques: Clinical Proteomics, Membrane

Journal: Journal of the Royal Society of Medicine

Article Title: A review of wearable technology in medicine

doi: 10.1177/0141076816663560

Figure Lengend Snippet: An overview of the available head-mounted display devices and their utilisation.

Article Snippet: HMM-3000MT (Sony Corporation, Japan) , Vision-based index finger tracking , Maintain sterility during teaching, easy to use, comfortable to wear, low cost and compact size , Lag between endoscopic image and pointer device , Yoshida et al. 11.

Techniques: Sterility, Imaging, Battery