Journal: Aging Cell

Article Title: Inhibition of the 60S ribosome biogenesis GTPase LSG1 causes endoplasmic reticular disruption and cellular senescence

doi: 10.1111/acel.12981

Figure Lengend Snippet: Knockdown of LSG1 leads to upregulation of cholesterol biosynthesis pathways and homeostatic alterations in the ER apparatus. (a) Gene set enrichment analysis (GSEA), ranked by normalized enrichment score (NES), revealed the top 5 upregulated biological processes as a result of LSG1 knockdown. The false discovery rate (FDR) yields the Q ‐value for statistical significance. (b) GSEA diagram of the cholesterol biosynthesis signature upon LSG1 knockdown as described in (a). (c) Western blot for LSG1, RPL28 and the cholesterol biosynthesis enzymes SQLE and HMGCS1 in shLSG1‐transduced MRC5 cells. (d) Immunofluorescence staining for calnexin in MRC5 cells transduced with control and with shLSG1, imaged by confocal microscopy. Scale bar: 50 μm. (e) High magnification images of the regions indicated in (d) stained for calnexin. (f) FIJI‐based analysis of the ER skeleton in the cells above, using the MiNA plugin (Valente et al., ). Error bars denote SEM of three biological replicates. Statistical significance is calculated using two‐tailed t tests. * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: Antibodies used were raised against: LSG1 (Proteintech 17750), EFL1 (24729), SBDS (Abcam ab128946), RPL28 (Proteintech 16649), BrdU (Pharmingen 558599), p53 (Santa Cruz sc‐126), p16 (Santa Cruz sc‐56330), p21 (Sigma p1484), pST/Q (Cell Signaling 2851), IL‐1α (R&D MAB200), IL‐1β (R&D MAB201), IL‐6 (R&D AF206NA), IL‐8 (R&D MAB208), Ki67 (Invitrogen 180191Z), calnexin Alexa Fluor 647 conjugate (Abcam ab202572), SQLE (Bethyl Laboratories A304‐590A‐T) and HMGCS1 (Proteintech 12544‐1‐AP).

Techniques: Knockdown, Western Blot, Immunofluorescence, Staining, Transduction, Control, Confocal Microscopy, Two Tailed Test

Journal: Journal of Nutritional Science

Article Title: Postprandial changes in gene expression of cholesterol influx and efflux mediators after intake of SFA compared with n -6 PUFA in subjects with and without familial hypercholesterolaemia: secondary outcomes of a randomised controlled trial

doi: 10.1017/jns.2019.25

Figure Lengend Snippet: Overview of the lipid-related genes examined by quantitative real-time PCR

Article Snippet: HMGCS1 , 3-Hydroxy-3-methylglutaryl-CoA synthase 1 , Cholesterol biosynthesis , Hs00940429_m1.

Techniques: Binding Assay

Journal: Journal of Nutritional Science

Article Title: Postprandial changes in gene expression of cholesterol influx and efflux mediators after intake of SFA compared with n -6 PUFA in subjects with and without familial hypercholesterolaemia: secondary outcomes of a randomised controlled trial

doi: 10.1017/jns.2019.25

Figure Lengend Snippet: Postprandial changes in gene expression in peripheral blood mononuclear cells that were significantly different between meals or groups * (Mean values with their standard errors)

Article Snippet: HMGCS1 , 3-Hydroxy-3-methylglutaryl-CoA synthase 1 , Cholesterol biosynthesis , Hs00940429_m1.

Techniques: Gene Expression

Journal: Journal of Nutritional Science

Article Title: Postprandial changes in gene expression of cholesterol influx and efflux mediators after intake of SFA compared with n -6 PUFA in subjects with and without familial hypercholesterolaemia: secondary outcomes of a randomised controlled trial

doi: 10.1017/jns.2019.25

Figure Lengend Snippet: Graphical summary. Hypothetical impact of postprandial gene expression in cholesterol homeostasis after SFA v. n -6 PUFA intake. Intake of SFA v. n -6 PUFA induces a larger reduction in the gene expression of LDL receptor ( LDLR ) and a lower increase of insulin-induced gene 1 ( INSIG1 ) which in combination may potentially result in decreased cholesterol influx, increased circulating cholesterol and increased cholesterol biosynthesis. Furthermore, intake of SFA v. n -6 PUFA induces larger increases in the gene expression of ATP-binding cassette, subfamily A, member 1 ( ABCA1 ) and ATP-binding cassette, subfamily G, member 1 ( ABCG1 ) which may potentially result in increased cholesterol efflux. Grey arrows indicate hypothetical impact of results. SCAP, sterol regulatory element binding cleavage activating protein; SREBP, sterol regulatory element binding protein; HMGCS1 , 3-hydroxy-3-methylglutaryl-CoA synthase 1; FH, familial hypercholesterolaemia; C, control; SREBF2 , sterol regulatory element binding transcription factor 2. The figure is based on free images from ServierMedical Art ( https://smart.servier.com) .

Article Snippet: HMGCS1 , 3-Hydroxy-3-methylglutaryl-CoA synthase 1 , Cholesterol biosynthesis , Hs00940429_m1.

Techniques: Gene Expression, Binding Assay, Control