hla dr5 Search Results


90
ATCC hla dr5
Hla Dr5, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson hla-dr-fitc clone g46-6 antibody
Hla Dr Fitc Clone G46 6 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Ricerche Srl human lymphoblastoid b cell line ebv-lu
Human Lymphoblastoid B Cell Line Ebv Lu, supplied by Ricerche Srl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l243  (ATCC)
95
ATCC l243
Autoreactivity of normal peripheral blood NKG2D + CD4 + T cells. (A) NKG2D + CD4 + T cells proliferate in the presence of CD25 + cell–depleted autologous PBMCs. CD3 + CD4 + CD8 − NKG2D + T cells FACSAria-sorted from magnetic bead–enriched CD4 + T cells to ≥99% purity were labeled with CFSE and cultured with irradiated autologous total or CD25 − stimulator PBMCs, or subpopulations thereof, as indicated. CD25 + lymphocytes were depleted to enhance T cell proliferation . Autoreactive NKG2D + CD4 + T cell stimulation is solely dependent on B cells as shown separately by B cell depletion and reconstitution. T cell and monocyte depletions have no effect. Blocking antibodies are specific for MHC class II (mAb TU39), and ULBP2 or ULBP3 (mAbs 6F6 and 4F9). The CFSE profiles shown represent gated NKG2D + CD4 + T cells. (B) Purified NKG2D − CD4 + T cells fail to proliferate in the presence of CD25 + cell–depleted autologous PBMCs. Treatment of NKG2D − CD4 + T cells with PHA is shown as positive control. CFSE profiles represent gated NKG2D − CD4 + T cells. Data shown in A and B are representative of results obtained in duplicate experiments with PBMCs from five independent donors. (C) Thymidine incorporation by NKG2D + CD4 + T cell clones in the presence of autologous, but not allogeneic, B cells is inhibited by anti-MHC class II (mAb TU39) and -HLA-DR (mAb <t>L243).</t> Clones 1–3 were derived from 3 donors and are representative of a total of 32 clones. Error bars indicate deviations among triplicate wells. Data shown are representative of three experiments per clone.
L243, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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90
Real Time Primers real-time primers
Autoreactivity of normal peripheral blood NKG2D + CD4 + T cells. (A) NKG2D + CD4 + T cells proliferate in the presence of CD25 + cell–depleted autologous PBMCs. CD3 + CD4 + CD8 − NKG2D + T cells FACSAria-sorted from magnetic bead–enriched CD4 + T cells to ≥99% purity were labeled with CFSE and cultured with irradiated autologous total or CD25 − stimulator PBMCs, or subpopulations thereof, as indicated. CD25 + lymphocytes were depleted to enhance T cell proliferation . Autoreactive NKG2D + CD4 + T cell stimulation is solely dependent on B cells as shown separately by B cell depletion and reconstitution. T cell and monocyte depletions have no effect. Blocking antibodies are specific for MHC class II (mAb TU39), and ULBP2 or ULBP3 (mAbs 6F6 and 4F9). The CFSE profiles shown represent gated NKG2D + CD4 + T cells. (B) Purified NKG2D − CD4 + T cells fail to proliferate in the presence of CD25 + cell–depleted autologous PBMCs. Treatment of NKG2D − CD4 + T cells with PHA is shown as positive control. CFSE profiles represent gated NKG2D − CD4 + T cells. Data shown in A and B are representative of results obtained in duplicate experiments with PBMCs from five independent donors. (C) Thymidine incorporation by NKG2D + CD4 + T cell clones in the presence of autologous, but not allogeneic, B cells is inhibited by anti-MHC class II (mAb TU39) and -HLA-DR (mAb <t>L243).</t> Clones 1–3 were derived from 3 donors and are representative of a total of 32 clones. Error bars indicate deviations among triplicate wells. Data shown are representative of three experiments per clone.
Real Time Primers, supplied by Real Time Primers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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N/A
HLA DRB5 belongs to the HLA class II beta chain paralogues This class II molecule is a heterodimer consisting of an alpha DRA and a beta DRB chain both anchored in the membrane It plays
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N/A
HLA DRB1 belongs to the HLA class II beta chain paralogs The class II molecule is a heterodimer consisting of an alpha DRA and a beta chain DRB both anchored in the membrane It plays
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N/A
Mouse anti Human HLA Class II DRB1 Antibody, Monoclonal (C1), could be used for WB and so on.Application:WB: 0.01-2 µg/mL
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Image Search Results


Autoreactivity of normal peripheral blood NKG2D + CD4 + T cells. (A) NKG2D + CD4 + T cells proliferate in the presence of CD25 + cell–depleted autologous PBMCs. CD3 + CD4 + CD8 − NKG2D + T cells FACSAria-sorted from magnetic bead–enriched CD4 + T cells to ≥99% purity were labeled with CFSE and cultured with irradiated autologous total or CD25 − stimulator PBMCs, or subpopulations thereof, as indicated. CD25 + lymphocytes were depleted to enhance T cell proliferation . Autoreactive NKG2D + CD4 + T cell stimulation is solely dependent on B cells as shown separately by B cell depletion and reconstitution. T cell and monocyte depletions have no effect. Blocking antibodies are specific for MHC class II (mAb TU39), and ULBP2 or ULBP3 (mAbs 6F6 and 4F9). The CFSE profiles shown represent gated NKG2D + CD4 + T cells. (B) Purified NKG2D − CD4 + T cells fail to proliferate in the presence of CD25 + cell–depleted autologous PBMCs. Treatment of NKG2D − CD4 + T cells with PHA is shown as positive control. CFSE profiles represent gated NKG2D − CD4 + T cells. Data shown in A and B are representative of results obtained in duplicate experiments with PBMCs from five independent donors. (C) Thymidine incorporation by NKG2D + CD4 + T cell clones in the presence of autologous, but not allogeneic, B cells is inhibited by anti-MHC class II (mAb TU39) and -HLA-DR (mAb L243). Clones 1–3 were derived from 3 donors and are representative of a total of 32 clones. Error bars indicate deviations among triplicate wells. Data shown are representative of three experiments per clone.

Journal: The Journal of Experimental Medicine

Article Title: Normally occurring NKG2D + CD4 + T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus

doi: 10.1084/jem.20081648

Figure Lengend Snippet: Autoreactivity of normal peripheral blood NKG2D + CD4 + T cells. (A) NKG2D + CD4 + T cells proliferate in the presence of CD25 + cell–depleted autologous PBMCs. CD3 + CD4 + CD8 − NKG2D + T cells FACSAria-sorted from magnetic bead–enriched CD4 + T cells to ≥99% purity were labeled with CFSE and cultured with irradiated autologous total or CD25 − stimulator PBMCs, or subpopulations thereof, as indicated. CD25 + lymphocytes were depleted to enhance T cell proliferation . Autoreactive NKG2D + CD4 + T cell stimulation is solely dependent on B cells as shown separately by B cell depletion and reconstitution. T cell and monocyte depletions have no effect. Blocking antibodies are specific for MHC class II (mAb TU39), and ULBP2 or ULBP3 (mAbs 6F6 and 4F9). The CFSE profiles shown represent gated NKG2D + CD4 + T cells. (B) Purified NKG2D − CD4 + T cells fail to proliferate in the presence of CD25 + cell–depleted autologous PBMCs. Treatment of NKG2D − CD4 + T cells with PHA is shown as positive control. CFSE profiles represent gated NKG2D − CD4 + T cells. Data shown in A and B are representative of results obtained in duplicate experiments with PBMCs from five independent donors. (C) Thymidine incorporation by NKG2D + CD4 + T cell clones in the presence of autologous, but not allogeneic, B cells is inhibited by anti-MHC class II (mAb TU39) and -HLA-DR (mAb L243). Clones 1–3 were derived from 3 donors and are representative of a total of 32 clones. Error bars indicate deviations among triplicate wells. Data shown are representative of three experiments per clone.

Article Snippet: T cell clones established in the presence of autologous B cells were tested for [ 3 H]thymidine incorporation after exposure to irradiated autologous or allogeneic B cells in the presence or absence of mAb TU39 (10 μg/ml) or L243 (anti-HLA-DR; 5 μg/ml; American Type Culture Collection).

Techniques: Labeling, Cell Culture, Irradiation, Cell Stimulation, Blocking Assay, Purification, Positive Control, Clone Assay, Derivative Assay