hk2 Search Results


human renal tubular cell line hk  (ATCC)
99
ATCC human renal tubular cell line hk
Human Renal Tubular Cell Line Hk, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human renal tubular cell line hk/product/ATCC
Average 99 stars, based on 1 article reviews
human renal tubular cell line hk - by Bioz Stars, 2026-03
99/100 stars
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gene exp hk2 mm00443385 m1  (Thermo Fisher)
94
Thermo Fisher gene exp hk2 mm00443385 m1
Gene Exp Hk2 Mm00443385 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
gene exp hk2 mm00443385 m1 - by Bioz Stars, 2026-03
94/100 stars
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hk2  (Proteintech)
97
Proteintech hk2
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Hk2, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hk2/product/Proteintech
Average 97 stars, based on 1 article reviews
hk2 - by Bioz Stars, 2026-03
97/100 stars
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pwzl neo myr flag hk2  (Addgene inc)
93
Addgene inc pwzl neo myr flag hk2
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Pwzl Neo Myr Flag Hk2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pwzl neo myr flag hk2/product/Addgene inc
Average 93 stars, based on 1 article reviews
pwzl neo myr flag hk2 - by Bioz Stars, 2026-03
93/100 stars
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anti hk2  (Proteintech)
96
Proteintech anti hk2
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Anti Hk2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti hk2/product/Proteintech
Average 96 stars, based on 1 article reviews
anti hk2 - by Bioz Stars, 2026-03
96/100 stars
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gene exp hk2 hs00606086 m1  (Thermo Fisher)
95
Thermo Fisher gene exp hk2 hs00606086 m1
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Gene Exp Hk2 Hs00606086 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp hk2 hs00606086 m1/product/Thermo Fisher
Average 95 stars, based on 1 article reviews
gene exp hk2 hs00606086 m1 - by Bioz Stars, 2026-03
95/100 stars
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anti kif2a  (Proteintech)
93
Proteintech anti kif2a
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Anti Kif2a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti kif2a/product/Proteintech
Average 93 stars, based on 1 article reviews
anti kif2a - by Bioz Stars, 2026-03
93/100 stars
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rabbit anti hk2  (Cusabio)
93
Cusabio rabbit anti hk2
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Rabbit Anti Hk2, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti hk2/product/Cusabio
Average 93 stars, based on 1 article reviews
rabbit anti hk2 - by Bioz Stars, 2026-03
93/100 stars
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pcmv6 xl5 vector  (OriGene)
90
OriGene pcmv6 xl5 vector
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Pcmv6 Xl5 Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv6 xl5 vector/product/OriGene
Average 90 stars, based on 1 article reviews
pcmv6 xl5 vector - by Bioz Stars, 2026-03
90/100 stars
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hexokinase 2  (OriGene)
92
OriGene hexokinase 2
Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of <t>β-catenin,HK2</t> and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group
Hexokinase 2, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hexokinase 2/product/OriGene
Average 92 stars, based on 1 article reviews
hexokinase 2 - by Bioz Stars, 2026-03
92/100 stars
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Image Search Results


Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of β-catenin,HK2 and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group

Journal: BMC cardiovascular disorders

Article Title: β-catenin mediates monocrotaline-induced pulmonary hypertension via glycolysis in rats.

doi: 10.1186/s12872-024-04000-z

Figure Lengend Snippet: Fig. 2 β-catenin enhances glycolysis and NLRP3 inflammasome activation in vitro. A Western blot showing the expression of β-catenin,HK2 and PKM2 in BMDMs. The blots were cropped and hybridized with the corresponding antibodies.Quantitative Western blot results were normalized to those of β-actin. B-D Glucose,PFK and LDH measurements in BMDMs. E Lactate determination in the cell supernatant. F Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. β-Actin was used as the internal reference. G-J TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + XAV939, LPS vs. LPS + LiCl, and LPS + XAV939 vs. LPS + XAV939 + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group

Article Snippet: The membranes were probed with antibodies against β-catenin (1:1000, Proteintech, Wuhan, China), HK2, PKM2 (1:4000, Proteintech, Wuhan, China), ASC, procaspase-1, caspase-1, NLRP3 (1:1000; all Cell Signaling Technology, USA), β-actin (1:2500, Proteintech, Wuhan, China).

Techniques: Activation Assay, In Vitro, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Control

Fig. 3 Enhanced glycolysis promotes the activation of the NLRP3 inflammasome in vitro. A The expression of HK2 and PKM2 in BMDMs. β-Actin was used as the internal reference. B-C The levels of the key glycolytic enzymes PFK and LDH were detected in vitro. D Lactate determination in the cell supernatant. E Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. The blots were cropped and hybridized with the corresponding antibodies. β-Actin was used as the internal reference. F-I TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + 2-DG, and LPS vs. LPS + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group

Journal: BMC cardiovascular disorders

Article Title: β-catenin mediates monocrotaline-induced pulmonary hypertension via glycolysis in rats.

doi: 10.1186/s12872-024-04000-z

Figure Lengend Snippet: Fig. 3 Enhanced glycolysis promotes the activation of the NLRP3 inflammasome in vitro. A The expression of HK2 and PKM2 in BMDMs. β-Actin was used as the internal reference. B-C The levels of the key glycolytic enzymes PFK and LDH were detected in vitro. D Lactate determination in the cell supernatant. E Representative Western blot showing the expression of NLRP3 inflammasome markers in BMDMs from the different groups. The blots were cropped and hybridized with the corresponding antibodies. β-Actin was used as the internal reference. F-I TNF-α, IL-6, IL-1β and IL-18 levels in the culture supernatants of BMDMs were measured by ELISA. The following comparisons were made: control vs. LPS, LPS vs. LPS + 2-DG, and LPS vs. LPS + FBP. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group

Article Snippet: The membranes were probed with antibodies against β-catenin (1:1000, Proteintech, Wuhan, China), HK2, PKM2 (1:4000, Proteintech, Wuhan, China), ASC, procaspase-1, caspase-1, NLRP3 (1:1000; all Cell Signaling Technology, USA), β-actin (1:2500, Proteintech, Wuhan, China).

Techniques: Activation Assay, In Vitro, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Control

Fig. 6 XAV939 suppressed glycolysis and NLRP3 inflammasome activation in vivo. A Western blot showing the expression of β-catenin,HK2,PKM2 in vivo. Quantitative Western blot results were normalized to those of β-actin. B The glucose content in lung tissue from the four groups of rats. C-E The contents of PFK, LDH and LA in vivo. F Representative Western blot showing the expression of NLRP3 inflammasome markers in lung samples from the different groups. The blots were cropped and hybridized with the corresponding antibodies. β-Actin was used as the internal reference. G-J The levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-18 in the lung tissue supernatants of the rats. The following comparisons were made: Sham vs. Sham + XAV939, Sham vs. MCT, and MCT vs. MCT + XAV939. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group

Journal: BMC cardiovascular disorders

Article Title: β-catenin mediates monocrotaline-induced pulmonary hypertension via glycolysis in rats.

doi: 10.1186/s12872-024-04000-z

Figure Lengend Snippet: Fig. 6 XAV939 suppressed glycolysis and NLRP3 inflammasome activation in vivo. A Western blot showing the expression of β-catenin,HK2,PKM2 in vivo. Quantitative Western blot results were normalized to those of β-actin. B The glucose content in lung tissue from the four groups of rats. C-E The contents of PFK, LDH and LA in vivo. F Representative Western blot showing the expression of NLRP3 inflammasome markers in lung samples from the different groups. The blots were cropped and hybridized with the corresponding antibodies. β-Actin was used as the internal reference. G-J The levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-18 in the lung tissue supernatants of the rats. The following comparisons were made: Sham vs. Sham + XAV939, Sham vs. MCT, and MCT vs. MCT + XAV939. The data are presented as the means ± SDs; ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; n = 3/group

Article Snippet: The membranes were probed with antibodies against β-catenin (1:1000, Proteintech, Wuhan, China), HK2, PKM2 (1:4000, Proteintech, Wuhan, China), ASC, procaspase-1, caspase-1, NLRP3 (1:1000; all Cell Signaling Technology, USA), β-actin (1:2500, Proteintech, Wuhan, China).

Techniques: Activation Assay, In Vivo, Western Blot, Expressing