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Immunohistochemical methods used in this study
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Immunohistochemical methods used in this study
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Immunohistochemical methods used in this study
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Immunohistochemical methods used in this study
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Immunohistochemical methods used in this study
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Image Search Results


Immunohistochemical methods used in this study

Journal: International Journal of Clinical and Experimental Pathology

Article Title: 8-hydroxydeguanosine and nitrotyrosine are prognostic factors in urinary bladder carcinoma

doi:

Figure Lengend Snippet: Immunohistochemical methods used in this study

Article Snippet: For Keap1, nrf2, prx 1-6 chromogen used was aminoethylcarbazole, for 8OHdG, nitrotyrosine and thioredoxin diaminobentzidine. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody (Clone/Product code) Dilution Immunostaining method Source of primary antibody PRDX I 1:1500 Histostain-Plus Bulk Kit Labfrontier, York, United Kingdom PRDX II 1:1000 Histostain-Plus Bulk Kit Labfrontier, York, United Kingdom PRDX III 1:500 Histostain-Plus Bulk Kit Labfrontier, York, United Kingdom PRDX IV 1:500 Histostain-Plus Bulk Kit Labfrontier, York, United Kingdom PRDX V 1:2000 Histostain-Plus Bulk Kit Labfrontier, York, United Kingdom PRDX VI 1:2000 Histostain-Plus Bulk Kit Labfrontier, York, United Kingdom TRX 1:200 A biotinylated secondary anti-goat antibody; avidin-biotin-peroxidase complex American Diagnostica, Greenwich, CT 8-OHdG 1:50 Dako Envision Kit JaICA, Fukuroi, Japan Nrf2 1:100 Histostain Plus Broad Spectrum Kit Santa Cruz Biotechnology, Santa Cruz, USA Keap1 1:100 Biocare Medical HRP Polymer Kit Santa Cruz Open in a separate window Immunohistochemical methods used in this study The immunohistochemical results were devided semiquantitatively as follows; 0=negative, 1-5 % = weak, 5-25 %=moderate, 25-75 %= strong, over 75 %= very strong.

Techniques: Immunohistochemical staining, Immunostaining, Avidin-Biotin Assay, Polymer