Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: IER3IP1-mutations cause microcephaly by selective inhibition of ER-Golgi transport

doi: 10.1007/s00018-024-05386-x

Figure Lengend Snippet:

Article Snippet: Cells grown on No.1 glass coverslips (0111550, Paul Marienfeld GmbH, Germany) were treated as indicated, then washed with 1xPBS, fixed with Roti R Histfix (P087.1, Carl Roth, Germany) (20 min at room temperature), permeabilized with 0.2% Triton X-100 in PBS (5 min), and blocked with a solution containing 1 ml FCS, 1 g BSA, 0.1 g fish gelatin in 100 ml 1xPBS for 20 min.

Techniques: Protease Inhibitor, Staining, Modification, Mutagenesis, Membrane, Labeling, Imaging