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96
Sino Biological recombinant protein
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Recombinant Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant protein/product/Sino Biological
Average 96 stars, based on 1 article reviews
recombinant protein - by Bioz Stars, 2026-03
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93
Novus Biologicals ybx1 recombinant protein
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Ybx1 Recombinant Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ybx1 recombinant protein/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
ybx1 recombinant protein - by Bioz Stars, 2026-03
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93
Danaher Inc spintrap columns
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Spintrap Columns, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spintrap columns/product/Danaher Inc
Average 93 stars, based on 1 article reviews
spintrap columns - by Bioz Stars, 2026-03
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92
BPS Bioscience recombinant arg1
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Recombinant Arg1, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant arg1/product/BPS Bioscience
Average 92 stars, based on 1 article reviews
recombinant arg1 - by Bioz Stars, 2026-03
92/100 stars
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93
Addgene inc pnsgrna plasmid
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Pnsgrna Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pnsgrna plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
pnsgrna plasmid - by Bioz Stars, 2026-03
93/100 stars
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95
Bio-Rad histidine
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Histidine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/histidine/product/Bio-Rad
Average 95 stars, based on 1 article reviews
histidine - by Bioz Stars, 2026-03
95/100 stars
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96
Sino Biological spike protein
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Spike Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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97
Sino Biological spike s1
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Spike S1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
spike s1 - by Bioz Stars, 2026-03
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97
Sino Biological numbers 40589 v08b1
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Numbers 40589 V08b1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/numbers 40589 v08b1/product/Sino Biological
Average 97 stars, based on 1 article reviews
numbers 40589 v08b1 - by Bioz Stars, 2026-03
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96
Proteintech 1 ig
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
1 ig - by Bioz Stars, 2026-03
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95
Zymo Research spin protein miniprep
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Spin Protein Miniprep, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spin protein miniprep/product/Zymo Research
Average 95 stars, based on 1 article reviews
spin protein miniprep - by Bioz Stars, 2026-03
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94
Sino Biological recombinant sars cov 2
Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using <t>recombinant</t> SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.
Recombinant Sars Cov 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant sars cov 2/product/Sino Biological
Average 94 stars, based on 1 article reviews
recombinant sars cov 2 - by Bioz Stars, 2026-03
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Image Search Results


Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using recombinant SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.

Journal: Analytical Chemistry

Article Title: Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein

doi: 10.1021/acs.analchem.0c02288

Figure Lengend Snippet: Figure 2. Schematic of the workflow used to develop a PRM assay for the detection and quantitation of SARS-CoV-2 spike and nucleoprotein A) PRM assay development was performed using recombinant SARS CoV-2 spike protein and nucleoprotein. Proteotypic target peptides/transitions were selected to generate a spectral library in Skyline. B) The PRM assay was then used to quantitate the SARS CoV-2 protein levels in a mock sample that was created by adding an inactivated virus sample to in-vitro derived mucus.

Article Snippet: SARS-CoV-2 nucleocapsid-His recombinant protein was purchased from Sino Biological (100 g, 40588-V08B,) The protein was difficult to dissolve and required treatment before S-trap preparation below with 40L dimethyl sulfoxide (276855-100mL, Sigma), 126 L 1% TFA, and 100L 1x S-Trap lysis buffer (5% SDS, 50 mM TEAB, pH adjusted to 7.55 using 12% phosphoric acid).

Techniques: Quantitation Assay, Recombinant, Targeted Proteomics, Virus, In Vitro, Derivative Assay