hindiii Search Results


hind iii hf restriction enzyme  (New England Biolabs)
96
New England Biolabs hind iii hf restriction enzyme

Hind Iii Hf Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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restriction endonucleases bamhi  (Jena Bioscience)
93
Jena Bioscience restriction endonucleases bamhi
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Restriction Endonucleases Bamhi, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hindiii  (New England Biolabs)
99
New England Biolabs hindiii
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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hindiii hf restriction enzymes  (New England Biolabs)
96
New England Biolabs hindiii hf restriction enzymes
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Hindiii Hf Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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anti pld3  (Proteintech)
93
Proteintech anti pld3
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Anti Pld3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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λdna hin diii digest  (Jena Bioscience)
90
Jena Bioscience λdna hin diii digest
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
λdna Hin Diii Digest, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e2f1 expression vector  (Addgene inc)
90
Addgene inc e2f1 expression vector
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
E2f1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bamhi ecori digested pcdna3  (Addgene inc)
94
Addgene inc bamhi ecori digested pcdna3
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Bamhi Ecori Digested Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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klenow dna polymerase end-filled bamhi/hindiii fragment from pabcluc  (Promega)
90
Promega klenow dna polymerase end-filled bamhi/hindiii fragment from pabcluc
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Klenow Dna Polymerase End Filled Bamhi/Hindiii Fragment From Pabcluc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kpni/hindiii polylinker restriction site  (Promega)
90
Promega kpni/hindiii polylinker restriction site
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Kpni/Hindiii Polylinker Restriction Site, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacteriophage dna/hindiii digest  (Promega)
90
Promega bacteriophage dna/hindiii digest
Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with <t>BamHI.</t> One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and <t>3;</t> <t>BamHI/HindIII</t> double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).
Bacteriophage Dna/Hindiii Digest, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Nature protocols

Article Title: Defining genome-wide CRISPR-Cas genome editing nuclease activity with GUIDE-seq

doi: 10.1038/s41596-021-00626-x

Figure Lengend Snippet:

Article Snippet: gRNA preparation RNAse ZAP solution (Fisher Scientific cat. no. AM9780) Plasmid pCRL1 for T7 in vitro transcription of gRNA (Addgene cat. no. 153997) Hind III-HF restriction enzyme (New England Biolabs cat. no. R3104S) HiScribe T7 RNA Synthesis Kit (New England Biolabs cat. no. E2050S) MEGAclear Transcription Clean-Up Kit (Fisher Scientific cat. no. AM1908) RNA High Sensitivity Screen Tape (Agilent cat. no 5067-5579) RNA High Sensitivity Sample Buffer (Agilent cat. no. 5067-5580) Mammalian Cell culture and nucleofection 24 Well Cell Culture Plate, Flat Bottom with Lid, Tissue Culture Treated, Non-Pyrogenic, Polystyrene (Corning Life Sciences cat. no. 3524) Non-Tissue Culture Treated Plate, 24 Well, Flat Bottom with Low Evaporation Lid (Corning Life Sciences cat. no. 351147) Non-Tissue Culture Treated Plate, 96 Well, Flat Bottom with Low Evaporation Lid (Corning Life Sciences cat. no. 351172) Cas9 expressing construct pSQT817 (Addgene cat. no. 53373) gRNA expression construct, backbone BPK1520 (Addgene cat. no. 65777) SE nucleofection kit (Lonza cat. no. V4SC-1096) Advanced DMEM (Fisher Scientific cat. no. 12491023) PBS (Fisher Scientific cat. no. 10010049) Disposable Hemocytometer (Fisher Scientific cat. no. 22600100) Trypan Blue (Fisher Scientific cat. no. 15250061) FBS, Fetal bovine serum (Fisher Scientific cat. no. 10-082-147) Trypsin-EDTA 0.05% (Fisher Scientific cat. no. 25300120) Penicillin/Streptomycin (Fisher Scientific cat. no. 15-070-063) GlutaMax 100X (Fisher Scientific cat. no. 35-050-061) 96 wells plate V-shape (Fisher Scientific cat. no. 720096) X-vivo 15 Culture Media (Lonza BEBP04-744Q) P3 nucleofection kit (Lonza V4SP-3096) !

Techniques: Concentration Assay, Plasmid Preparation

Journal: Nature protocols

Article Title: Defining genome-wide CRISPR-Cas genome editing nuclease activity with GUIDE-seq

doi: 10.1038/s41596-021-00626-x

Figure Lengend Snippet:

Article Snippet: gRNA preparation RNAse ZAP solution (Fisher Scientific cat. no. AM9780) Plasmid pCRL1 for T7 in vitro transcription of gRNA (Addgene cat. no. 153997) Hind III-HF restriction enzyme (New England Biolabs cat. no. R3104S) HiScribe T7 RNA Synthesis Kit (New England Biolabs cat. no. E2050S) MEGAclear Transcription Clean-Up Kit (Fisher Scientific cat. no. AM1908) RNA High Sensitivity Screen Tape (Agilent cat. no 5067-5579) RNA High Sensitivity Sample Buffer (Agilent cat. no. 5067-5580) Mammalian Cell culture and nucleofection 24 Well Cell Culture Plate, Flat Bottom with Lid, Tissue Culture Treated, Non-Pyrogenic, Polystyrene (Corning Life Sciences cat. no. 3524) Non-Tissue Culture Treated Plate, 24 Well, Flat Bottom with Low Evaporation Lid (Corning Life Sciences cat. no. 351147) Non-Tissue Culture Treated Plate, 96 Well, Flat Bottom with Low Evaporation Lid (Corning Life Sciences cat. no. 351172) Cas9 expressing construct pSQT817 (Addgene cat. no. 53373) gRNA expression construct, backbone BPK1520 (Addgene cat. no. 65777) SE nucleofection kit (Lonza cat. no. V4SC-1096) Advanced DMEM (Fisher Scientific cat. no. 12491023) PBS (Fisher Scientific cat. no. 10010049) Disposable Hemocytometer (Fisher Scientific cat. no. 22600100) Trypan Blue (Fisher Scientific cat. no. 15250061) FBS, Fetal bovine serum (Fisher Scientific cat. no. 10-082-147) Trypsin-EDTA 0.05% (Fisher Scientific cat. no. 25300120) Penicillin/Streptomycin (Fisher Scientific cat. no. 15-070-063) GlutaMax 100X (Fisher Scientific cat. no. 35-050-061) 96 wells plate V-shape (Fisher Scientific cat. no. 720096) X-vivo 15 Culture Media (Lonza BEBP04-744Q) P3 nucleofection kit (Lonza V4SP-3096) !

Techniques: Concentration Assay, Plasmid Preparation

Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with BamHI. One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; BamHI/HindIII double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).

Journal: Avicenna Journal of Medical Biotechnology

Article Title: Molecular characterization and Functional Analysis of the PilQ 380-706 : a Novel Secretin Domain in Pseudomonas aeruginosa

doi:

Figure Lengend Snippet: Agarose gel electrophoresis analysis of recombinant pET28a/ pilQ 1138-2118 with restriction enzyme digestion. Lane M; DNA marker (1 kb ), Lane 1; mono-digestion of the pET28a/ pilQ 1138-2118 vector with BamHI. One expected fragment was observed on the gel (∼6350 bp band). Lane 2 and 3; BamHI/HindIII double digested the recombinant vector with BamHI and HindIII buffer, respectively. Two expected fragments from double digestion were observed on the gel (∼5369 and 981 bp bands). Lane 4; the optimized PCR product of the pilQ 1138-2118 gene (∼ 961 bp band).

Article Snippet: The vector was treated with the restriction endonucleases BamHI and HindIII (Jena Bioscience Kit, Germany) according to manufacture instruction.

Techniques: Agarose Gel Electrophoresis, Recombinant, Marker, Plasmid Preparation