Journal: PLoS ONE

Article Title: An Integrated Physical, Genetic and Cytogenetic Map of Brachypodium distachyon , a Model System for Grass Research

doi: 10.1371/journal.pone.0013461

Figure Lengend Snippet: Characteristics of the two BAC libraries used to construct the Brachypodium physical map.

Article Snippet: The Hin dIII library (BD_ABa) consisted of 36,864 clones with an average insert size of 128 Kb , and the Eco RI library (BD_CBa) consisted of 36,864 clones with an average insert size of 124 Kb ( ).

Techniques: Construct

Journal: Frontiers in Plant Science

Article Title: Genetic and morphological variants of Acidovorax avenae subsp. avenae cause red stripe of sugarcane in China

doi: 10.3389/fpls.2023.1127928

Figure Lengend Snippet: In silico analysis of RFLP patterns of 17 strains of Acidovorax avenae subsp. avenae from sugarcane in China based on the internal transcribed spacer (ITS) region of 16S-23S rDNA.

Article Snippet: A restriction fragment length polymorphism (RFLP) analysis of the Aaa sequences obtained with primers RS-ITS-F1/RS-ITS-R1 was performed in silico using restriction enzymes Hin dIII and Eco RI with DNAMAN software (Lynnon Biosoft, San Ramon, USA).

Techniques: In Silico, Sequencing

Journal:

Article Title: Cytochrome P450 Epoxygenase Metabolism of Arachidonic Acid Inhibits Apoptosis

doi: 10.1128/MCB.21.18.6322-6331.2001

Figure Lengend Snippet: 14,15-EET exerts its antiapoptotic effects in LLCPKcl4 cells by activation of a PI-3 kinase–Akt-signaling pathway. (A) 14,15-EET's antiapoptotic effects were abolished by inhibition of PI-3 kinase but not by inhibition of MAP kinase. After pretreatment with or without the specific PI-3 kinase inhibitor wortmannin (10 nM) or LY294002 (5 μM) or the MEK inhibitor PD98059 (10 μM) for 30 min, followed by 14,15-EET or vehicle for 1 h, LLCPKcl4 cells were exposed to H2O2 for 24 h, and DNA laddering was assessed. (B) Arachidonic acid's antiapoptotic effects in BM3 cells were abolished by inhibition of PI-3 kinase but not by inhibition of MAP kinase. Before induction of H2O2-mediated apoptosis, BM3 cells were pretreated with or without wortmannin, LY294002, or PD98059 and then treated with or without arachidonic acid. (A and B) Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA-HindIII size marker. (C) 14,15-EET increased Akt kinase activity, which was abolished by either wortmannin or LY294002. After treatment with or without the specific PI-3 kinase inhibitor wortmannin (10 nM) or LY294002 (5 μM), LLCPKcl4 cells were treated with or without 14,15-EET. The cells were then lysed and subjected to immunoprecipitation and Akt kinase activity assay with histone H2B as a substrate.

Article Snippet: Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA- Hin dIII DNA size marker (Promega). (C) 14,15-EET decreased H 2 O 2 - or etoposide-induced apoptotic-cell number; cells were scored for morphological evidence of apoptosis as described in Materials and Methods.

Techniques: Activation Assay, Inhibition, DNA Laddering, Lambda DNA Preparation, Marker, Activity Assay, Immunoprecipitation, Kinase Assay

Journal:

Article Title: Cytochrome P450 Epoxygenase Metabolism of Arachidonic Acid Inhibits Apoptosis

doi: 10.1128/MCB.21.18.6322-6331.2001

Figure Lengend Snippet: Antiapoptotic effects of exogenously administered 14,15-EET in LLCPKcl4 cells. (A) 14,15-EET prevented the renal epithelial cell line LLCPKcl4 from progressive loss of cell viability caused by serum deprivation. LLCPKcl4 cells grown to confluence were changed to serum-free medium. After 48 h, vehicle (Me2SO) or 14,15-EET (10 μM) was added every 3 days. After 10 days, the majority of the vehicle-treated cells had died while the 14,15-EET treated cells still maintained a confluent monolayer with normal morphology. (B) 14,15-EET inhibited DNA laddering induced by H2O2 or etoposide. After pretreatment with vehicle (Me2SO) or 14,15-EET (10 μM) for 1 h, quiescent LLCPKcl4 cells were exposed to H2O2 (500 μM) or etoposide (100 μM) for 24 h, and genomic DNA was extracted and electrophoresed on a 1.5% agarose gel containing ethidium bromide. Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA-HindIII DNA size marker (Promega). (C) 14,15-EET decreased H2O2- or etoposide-induced apoptotic-cell number; cells were scored for morphological evidence of apoptosis as described in Materials and Methods.

Article Snippet: Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA- Hin dIII DNA size marker (Promega). (C) 14,15-EET decreased H 2 O 2 - or etoposide-induced apoptotic-cell number; cells were scored for morphological evidence of apoptosis as described in Materials and Methods.

Techniques: DNA Laddering, Agarose Gel Electrophoresis, Lambda DNA Preparation, Marker

Journal:

Article Title: Cytochrome P450 Epoxygenase Metabolism of Arachidonic Acid Inhibits Apoptosis

doi: 10.1128/MCB.21.18.6322-6331.2001

Figure Lengend Snippet: Effects of endogenously produced 14,15-EET in LLCPKcl4 cells. Arachidonic acid blocked H2O2-induced apoptosis in BM3 cells but not in Vector cells. Quiescent BM3 cells and Vector cells were pretreated with or without arachidonic acid (10 μM) for 1 h before exposure to H2O2 for 24 h, and DNA laddering (A) and apoptotic cell number (B) were assessed as described in Materials and Methods. (A) Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA-HindIII DNA size marker.

Article Snippet: Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA- Hin dIII DNA size marker (Promega). (C) 14,15-EET decreased H 2 O 2 - or etoposide-induced apoptotic-cell number; cells were scored for morphological evidence of apoptosis as described in Materials and Methods.

Techniques: Produced, Plasmid Preparation, DNA Laddering, Lambda DNA Preparation, Marker

Journal:

Article Title: Cytochrome P450 Epoxygenase Metabolism of Arachidonic Acid Inhibits Apoptosis

doi: 10.1128/MCB.21.18.6322-6331.2001

Figure Lengend Snippet: Arachidonic acid induced apoptosis in Vector cells but not in BM3 cells. Quiescent BM3 cells and Vector cells were treated with or without arachidonic acid (10 μM) for 48 h in the absence of other apoptosis-inducing stimuli and then subjected to assessment of DNA laddering. Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA-HindIII size marker.

Article Snippet: Leftmost lane, 100-bp DNA ladder; rightmost lane, lambda DNA- Hin dIII DNA size marker (Promega). (C) 14,15-EET decreased H 2 O 2 - or etoposide-induced apoptotic-cell number; cells were scored for morphological evidence of apoptosis as described in Materials and Methods.

Techniques: Plasmid Preparation, DNA Laddering, Lambda DNA Preparation, Marker