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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Akt Mediates Insulin-stimulated Phosphorylation of Ndrg2
doi: 10.1074/jbc.m401504200
Figure Lengend Snippet: FIG. 1. Phosphorylation of rat skel- etal muscle fractions in vitro with re- combinant PKC. Crude solubilized membrane fractions from rat skeletal muscle (A) or fractions partly purified by Mono Q (B) or ceramic hydroxyapatite chromatography (C) were incubated in the absence (C) or presence of recombi- nant PKC () or PKC () and [-32P]ATP as described under “Experimental Proce- dures.” Phosphorylated proteins were subjected to SDS-PAGE and phosphorim- aging. Autophosphorylation of recombi- nant PKC (rec ) and PKC (rec ) was also determined in the absence of skeletal muscle fractions (D). The migration of molecular mass markers and pp48 (*) are indicated to the left of the corresponding phosphorimaging analysis image.
Article Snippet: Macroprep High Q resin,
Techniques: Phospho-proteomics, In Vitro, Membrane, Purification, Chromatography, Incubation, SDS Page, Migration