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Image Search Results
Journal: Experimental & Molecular Medicine
Article Title: Differentiation of human gingival mesenchymal stem cells into neuronal lineages in 3D bioconjugated injectable protein hydrogel construct for the management of neuronal disorder
doi: 10.1038/emm.2015.113
Figure Lengend Snippet: Morphology of cultured gingival cells from gingival tissue. The phase-contrast micrographs of gingival cells on day 1 ( a ), day 5 ( b ), day 7 ( c ) and day 18 ( d ) of primary culture show a small and large colony composed of densely distributed spindle (black arrow)-shaped cells. On day 15, the cells became flattened and surrounded by proliferating cells and reached confluency. Scale bar =50 μm. Original magnifications × 200. The morphology of the colony formation of HGMSCs represents that P2 cells stained with 1% crystal violet in methanol at day 15 of culture ( e, f ) show CFU-F colonies. Scale bar=20 cm. Original magnifications × 100. The proliferative effect of HGMSCs in vitro was measured by 3 H-thymidine incorporation assay. ( g ) The amount of incorporated 3 H-thymdine was measured after 24 h of culturing. Bars represent the mean±s.e.m. ( n =3). The flow cytometry analysis of HGMSCs, passage 3. ( h ) The expression is strongly positive for MSC-specific surface markers CD105, CD90, CD73 and negative for CD34 and CD45, compared with Isotype control, thus confirming their identity as MSCs. A histogram represents the expression level percentage. The mesenchymal-lineage differentiations of HGMSCs into osteocytes, adipocytes and chondrocytes. In vitro differentiated cells showed positive staining in the specific staining methods. ( i ) The deposition of a mineralized extracellular matrix (red) was seen after alizarin red staining. Adipogenic differentiation was confirmed by the Oil Red O staining of intracytoplasmic lipid droplets. The chondrogenic differentiation of MSCs with the positive staining of proteoglycans by Alcian blue; scale bar =20 μm. Original magnification × 200.
Article Snippet:
Techniques: Cell Culture, Staining, In Vitro, Thymidine Incorporation Assay, Flow Cytometry, Expressing, Control