Journal: BioMed Research International

Article Title: Astaxanthin Enhances Gingival Wound Healing following High Glucose-Induced Oxidative Stress

doi: 10.1155/2022/4043105

Figure Lengend Snippet: Effects of ASTX on ROS levels induced by hyperglycemia in human gingival fibroblasts were determined by confocal microscopy. Representative images of DCFDA from 5-, 25-, and 50 mM glucose and ASTX-treated groups are given. To monitor the ROS levels, DCFDA loaded cells were calibrated with 100 μ M H 2 O 2 . Changes in total fluorescent intensity in LG group were higher than HG groups, as well as ASTX-treated group ( p < 0.001). ROS levels were decreased in the ASTX group when compared to the 50 mM HG group ( p < 0.001). Scale bar: 80 μ m.

Article Snippet: Human gingival fibroblasts (Accegen Biotechnology, Fairfield, New Jersey, USA, Cat no: ABC-TC3627) were incubated in low glucose (LG) conditions; in a 1 : 1 mixture of Dulbecco's Modified Eagle's Medium/Nutrient Mixture Ham's F-12 (DMEM/F12) containing 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml- μ g/ml penicillin-streptomycin (PS), and 2.5 mg/ml amphotericin B (AMP-B) in a humidified atmosphere of 5% CO 2 at 37°C.

Techniques: Confocal Microscopy

Journal: Stem Cells and Development

Article Title: MicroRNA-200c Attenuates Periodontitis by Modulating Proinflammatory and Osteoclastogenic Mediators

doi: 10.1089/scd.2019.0027

Figure Lengend Snippet: miR-200c protects LPS-induced ABL in rat periodontitis. Rats were injected bi-weekly with 10 μg LPS and treated weekly with one injection of PBS, PEI facilitating EV (1 and 10 μg), miR-200c (1 and 10 μg) up to 4 weeks. (A) Three-dimensional reconstructed micro-computed tomography images of maxillary alveolar bone on the palatal sides of rats 4 weeks after receiving an LPS injection with and without miR-200c. Red dot lines indicate the ABC and CEJ at maxillary second molars. Scale bars: 1 mm. (B, C) The outcome of the ABL (B) and the inter-radicular space (C) at the M2 after 4 weeks. (D, E) Structural parameters of alveolar bone 4 weeks after different treatment, including (Tb. Th) (D), and the percentages of BV/TV, BS/BV, and the total alveolar bone porosity (E). *P < 0.05 versus untreated; #P < 0.05 versus LPS-PBS; n = 8 for each treatment. ABC, alveolar bone crest; ABL, alveolar bone loss; BS, bone surface; BV, bone volume; CEJ, cemento-enamel junction; M2, second maxillary molar; PBS, phosphate-buffered saline; Tb. Th, trabecular thickness; TV, tissue volume. Color images are available online.

Article Snippet: As the resident cells in periodontitis, primary HGFs (Sciencell, Carlsbad, CA) were used to test the inhibitory function of miR-200c in proinflammatory and osteoclastogenic mediators.

Techniques: Injection, Micro-CT, Saline

Journal: Stem Cells and Development

Article Title: MicroRNA-200c Attenuates Periodontitis by Modulating Proinflammatory and Osteoclastogenic Mediators

doi: 10.1089/scd.2019.0027

Figure Lengend Snippet: Histological analysis of alveolar bone and periodontal ligament adjacent to the M2 of 4 weeks LPS-treated rats. (A) and (B) Representative micrographs of sham and periodontitis rats treated with PBS, PEI facilitating EV (1 and 10 μg), and miR-200c (1 and 10 μg) with hematoxylin and eosin staining (A) and Masson's trichrome staining (B). Magnification = 4. (C) Ratio of the remaining ACB to the RL adjacent to the M2. The ratio of the ACB to the RL was calculated by using the formula (ABC-RA)/(CEJ-RA), where ABC-RA is the distance from ABC to RA and CEJ-RA is the distance from CEJ to RA. *P < 0.05 vs untreated; #P < 0.05 versus LPS-PBS; n = 3 for each treatment. ACB, alveolar crest bone; RA, root apex; RL, root length. Color images are available online.

Article Snippet: As the resident cells in periodontitis, primary HGFs (Sciencell, Carlsbad, CA) were used to test the inhibitory function of miR-200c in proinflammatory and osteoclastogenic mediators.

Techniques: Staining

Journal: Stem Cells and Development

Article Title: MicroRNA-200c Attenuates Periodontitis by Modulating Proinflammatory and Osteoclastogenic Mediators

doi: 10.1089/scd.2019.0027

Figure Lengend Snippet: Potential mechanism(s) of anti-inflammatory/osteoclastogenic function of miR-200c to attenuate periodontitis.

Article Snippet: As the resident cells in periodontitis, primary HGFs (Sciencell, Carlsbad, CA) were used to test the inhibitory function of miR-200c in proinflammatory and osteoclastogenic mediators.

Techniques: